Pnpt1 (BC055826) Mouse Untagged Clone

CAT#: MC220827

Pnpt1 (untagged) - Mouse polyribonucleotide nucleotidyltransferase 1 (cDNA clone MGC:67745 IMAGE:3498934), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Pnpt1
• Synonyms: Old35, PNPase, RP23-313I4.2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >BC055826
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGGCCTGCAGGCTGTGCTGCTTGTGCCCGTGCCTCCGACCGCTGGGCTGCGGCCCCCTCGGCCGGC
  CCGGGCGGAATCGGGCACTCAGCTATTTGCAGATGCGAGCGTTGTGGAGCAGCACCGGATCCAGAGCGGT
  GACTGTAGACCTGGGACACAGAAAATTAGAAATATCCTCTGGGAAACTGGCAAGATTTGCTGATGGCTGT
  GCTGTGATACAGTCAGGCGATACTGCAGTAATGGTCACGGCAGTCAGCAAAACAAAAGCTTCGCCTTCCC
  AATTCATGCCGTTGGTGGTGGACTACCGACAGAAGGCTGCTGCAGCAGGCAGAATCCCCACAAACTACCT
  TAGAAGGGAGATTGGCTCCTCTGACAGAGAGGTTCTTACAAGTCGAGTAATAGATCGTTCAATTCGCCCT
  CTCTTTCCAGCCGGCTATTTTTATGATACTCAGGTTCTCTGTAATCTGCTAGCAGTAGATGGCATCAATG
  AACCTGATATCCTAGCAGTTAATGGTGCTTCTGTAGCCCTCTCCTTATCAGATATCCCTTGGAATGGACC
  TGTTGGGGCAGTACGAATAGGAATGATTGATGGAGAATGTGTCGTTAACCCAACAAGGAGAGAAATGTCT
  TCTAGCACTTTAAATTTAGTAGTTGCCGGAGCACCTAAAAGCCAAATTGTTATGTTGGAAGCCTCTGCAG
  AAAATATTCTACAGCAGGACTTTTGCCATGCTATCAAAGTTGGGGTGAAGTATACACAGCAGATAATTCA
  AGGCATCCAGCAGTTGGTAAAAGAAATCGGTGTTGCCAAGAGGACACCGCAGAAGATATTTACTCCTTCT
  GCAGAGATTGTGAAGTACACGAAGATAATTGCCATGGAGAAACTCTATGCGGTTTTTACAGATTATGAAC
  ATGATAAAGTTTCCAGGGATGAAGCTGTTAACAAGATAAGATTAGATACAGAGGAGCATCTAAAGGAAAA
  ATTTCCAGAGGTTGACCAATTTGAAATAATAGAATCCTTCAACATTGTTGCAAAGGAGGTTTTCCGAAGT
  ATTATTTTGAATGAATACAAAAGGTGTGATGGAAGAGATCTGACTTCACTTAGGAATATAAGTTGTGAGG
  TCGATATGTTTAAAACACTTCATGGATCAGCATTATTTCAGAGAGGACAAACACAGGTACTCTGTACTGT
  TACATTTGATTCATTAGAATCCAGTATTAAGTCAGATCAAATTATAACAGCTATAAATGGGGTAAAAGAT
  AAAAATTTCATGCTGCACTATGAGTTCCCTCCTTATGCAACCAATGAAACTGGCAAAGTTACTGGTGTGA
  ATCGAAGAGAACTTGGACATGGTGCTCTTGCCGAGAAAGCTTTGTGTCCTGTTATTCCCAAAGATTTTCC
  TTTTACCATAAGAGTTACATCTGAAGTCCTCGAATCAAATGGGTCATCTTCTATGGCATCTGCATGTGGT
  GGAAGTTTGGCATTAATGGATGCAGGGGTCCCAATTTCATCTGCTGTTGCAGGTGTAGCAGTGGGATTGG
  TTACCAAAACCAATCCAGAGAAAGGTGAAATAGAAGACTACCGTTTGCTAACAGATATTCTGGGAATTGA
  AGATTATAATGGTGACATGGATTTCAAAATAGCCGGTACAAATAAAGGAATAACTGCATTACAGGCTGAT
  ATTAAGTTACCTGGAGTACCAATTAAAATTATAATGGAAGCCATCCAACAAGCGTCAGTGGCAAAGAAGG
  AGATACTGCAGATAATGAACAAAACGATTTCAAAACCTCGAGCATCAAGAAAAGAAAATGGACCAGTTGT
  AGAAACAGTAAAGGTTCCATTATCAAAACGAGCAAAATTCGTTGGGCCTGGTGGATATCACTTAAAAAAA
  CTCCAGGCTGAGACAGGTGTAACAATTAGTCAGGTTGATGAAGAAACCTTCTCCATATTTGCACCAACAC
  CTACTGCAATGCATGAAGCAAGAGATTTCATTACAGAAATTTGCAGAGATGATCAAGAGCAACAATTAGA
  ATTTGGAGCAGTTTATACCGCGACAATAACTGAAATCAGAGACACTGGAGTGATGGTAAAACTGTATCCA
  AACATGACTGCAGTGCTGCTTCATAATTCACAACTTGACCAACGAAAGATTAAACATCCCACTGCCCTAG
  GACTAGAGGTTGGCCAAGAAATTCAGGTCAAATACTTTGGCCGTGATCCAGCTGATGGAAGAATGAGGCT
  TTCTCGTAAAGTACTTCAGTCTCCAGCTACAACTGCTCTCAAAACTCTAAATGATAGAAGCAGCATTGTA
  ATGGGAGAGCCTGTCTCACAGTCATCTAACTCTAACCCTTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCTGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: BC055826
• Insert Size: 2352 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC055826, AAH55826
• RefSeq Size: 2598 bp
• RefSeq ORF: 2351 bp
• Locus ID: 71701
• Cytogenetics: 11 A3.3
• Gene Summary: RNA-binding protein implicated in numerous RNA metabolic processes. Catalyzes the phosphorolysis of single-stranded polyribonucleotides processively in the 3'-to-5' direction. Mitochondrial intermembrane factor with RNA-processing exoribonulease activity. Component of the mitochondrial degradosome (mtEXO) complex, that degrades 3' overhang double-stranded RNA with a 3'-to-5' directionality in an ATP-dependent manner. Required for correct processing and polyadenylation of mitochondrial mRNAs. Plays a role as a cytoplasmic RNA import factor that mediates the translocation of small RNA components, like the 5S RNA, the RNA subunit of ribonuclease P and the mitochondrial RNA-processing (MRP) RNA, into the mitochondrial matrix. Plays a role in mitochondrial morphogenesis and respiration; regulates the expression of the electron transport chain (ETC) components at the mRNA and protein levels. In the cytoplasm, shows a 3'-to-5' exoribonuclease mediating mRNA degradation activity; degrades c-myc mRNA upon treatment with IFNB1/IFN-beta, resulting in a growth arrest in melanoma cells. Regulates the stability of specific mature miRNAs in melanoma cells; specifically and selectively degrades miR-221, preferentially. Plays also a role in RNA cell surveillance by cleaning up oxidized RNAs. Binds to the RNA subunit of ribonuclease P, MRP RNA and miR-221 microRNA.[UniProtKB/Swiss-Prot Function]