Rbak (BC059913) Mouse Untagged Clone

CAT#: MC220804

Rbak (untagged) - Mouse RB-associated KRAB repressor (cDNA clone MGC:69906 IMAGE:6825552), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Rbak
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >BC059913
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCGTTGCAGGGCCCACTGTCATTCAAGGATGTGGCTGTGGCCTTCTCCCAGGAGGAGTGGCAGCAGC
  TGGACCCTGAGGAGAGGACCACATACAGGGATGTGATGCTGGAGACCTACAGCAACCTCGTCTCCGTGGG
  GTATGACGTTACCAAGCCAAACATGATTATTAAGTTGGAGCAGGGAGAAGAGCCCTGGACAGTGGAAGGT
  GACCGCCATGCTCAGAGGCATTTGGAAATCAGTAAAGTTTATGACCCCAGAGAAGGAATTGAGGAAATTG
  GCGAGAAACATCTGCAGTGCGATGATGATCCCTACTGCTGGAGAGCTGAGAAGGGGGCTGCTTTTGATGA
  AGCCTACACCCTCGAAACAGCCCTCATTTCCCCCAGCTCAGGTGCTCACAGCTGTGTCTCCTGTGGGGAG
  ACCCTGGAGTCTGTTTCAGAACTGATTAGCAGTGACGGAAGCTATGCACTGGAGAAGCCCAGCATGTGTT
  TTGAGTGTGGGAAAGCATACGGAGAAAGCCTTGAAGACTTCAACCAAGATGAGGGTAACTCCTCTCAACA
  CGATGAAAATATTTTGCAGAAAGTCACCATTTTGGAGAAACCCTTTGCTTATGAGTGCATGGAAGCCTTA
  GACAGTGAAAGTGTTTTCATGGCTCGTGAGAGAGCTTACATGGGGGAGAAGCCCTATGACTGGGGAGACT
  CTGGACCAGACTTCATCCAGATGTCGGACTTCAGTACATATCCGAGATCACAAATGGAACTGAAGCCCTT
  CGAATGTACACAGTGTGGGAAGTCCTTCTGTAAGAAGTCCAAATTCATCATCCACCAGAGAGCTCACACA
  GGAGAGAAGCCGTACGCTTGTAGTGTGTGTGGAAAGTCCTTCAGTCAAAAGGGAACCCTCACTGTACATC
  GGAGATCGCACTTAGAGGAGAAGCCCTATAAGTGTAATGAGTGTGGGAAGACCTTTTGTCAGAAGTTACA
  CCTCACACAGCACCAGAGGACTCACTCAGGAGAGAAGCCCTATGAATGTAGCGAGTGCGGGAAGTCCTTC
  TGCCAGAAGACACACCTCACTCTCCACCAGAGGAATCACTCAGGCGAGAGGCCCTACCCGTGTAATGAAT
  GTGGGAAATCCTTCTCCCGGAAGTCTGCCCTCAATGACCATCAGAGGACACACACAGGAGAGAAGCTCTA
  TAAGTGTAATGAGTGTGGGAAATCCTACTACCGGAAGTCCACCCTCATTACACACCAGAGGACACACACA
  GGGGAGAAGCCCTATCAGTGTAGTGAGTGTGGGAAATTCTTCTCTCGGGTGTCGTACCTCACCATACACT
  ATCGGAGCCATTTAGAAGAGAAACCTTACGAGTGTACAGAATGTGGGAAAACCTTCAATCTAAACTCTGC
  TTTCATTAGGCACTGGAAGGTCCATGCAGAAGAGAGAGTCCAGGAGTGTGGCGAGTGTGGGAAGCCATCC
  CCGCTGCAGTGTGCCCCTGATCACACAGGCGACCTAGGAGAGAAGCGCTATGAGTGTAATGAGTGTGGAA
  AGACCTTCCTTGACAGCTCGGCCTTCCATAGGCACCAGTCCGTCCCTGAAGGGGAGAAAACTTACGAGTG
  TAATATATGCGGAAAGTCCTTCTCGGATTCGTCATGCTACACTGTGCACTACAGAGGTCATTCCGAAGAG
  AAGCCCTTCGGGTGCAGTGAATGTGGGAAGACCTTCTCTCATAACTCATCCCTCTTCAGACATCAAAGGG
  TCCACACCGGCGAGAAGCCGTACGAGTGTTACGAATGTGGGAAGTTCTTCTCTCAGAAGTCGTATCTCAC
  TATCCATCACCGGATCCATTCGGGAGAGAAGCCCTACGAGTGCAGCAAGTGTGGGAAAGTGTTCTCGCGG
  ATGTCGAACCTCACCGTACACTACCGAAGCCATTCAGGAGAGAAGCCGTATGAGTGCAACGAGTGTGGGA
  AAGTCTTTTCTCAGAAGTCGTACCTCACCGTGCATTACCGGACTCATTCAGGAGAGAAACCTTATGAATG
  TAACGAATGTGGGAAAAAATTCCACCACAGATCAGCCTTCAATAGCCATCAGAGAATTCATAAAAGAGGC
  ACTGTAAATGTACTCACTGTAGAAAAGCTCTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCTGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: BC059913
• Insert Size: 174 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC059913
• RefSeq Size: 3256 bp
• RefSeq ORF: 2132 bp
• Locus ID: 57782
• Cytogenetics: 5 G2
• Gene Summary: May repress E2F-dependent transcription. May promote AR-dependent transcription.[UniProtKB/Swiss-Prot Function]