Rps6ka1 (BC094470) Mouse Untagged Clone

CAT#: MC220754

Rps6ka1 (untagged) - Mouse ribosomal protein S6 kinase polypeptide 1 (cDNA clone MGC:102570 IMAGE:5098650), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Rps6ka1
• Synonyms: Rsk1, rsk, p90rsk
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >BC094470
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCCGCTCGCCCAGCTCAAGGAGCCCTGGCCGCTCATGGAGCTGGTGCCGCTGGACCCGGAGAATGGCC
  AGACTTCAGGGGAAGAAGCTGGACTTCAGCCATCCAAGGATGAGGCCATCCTCAAGGAGATCTCCATCAC
  ACACCACGTCAAGGCTGGCTCTGAGAAGGCCGATCCATCCCAGTTTGAGCTCCTCAAGGTTCTGGGCCAA
  GGATCCTTTGGCAAAGTCTTCCTGGTACGCAAGGTCACCCGGCCTGACAGTGGGCACTTGTATGCCATGA
  AAGTATTAAAGAAGGCCACGCTGAAAGTGCGTGACCGTGTTCGGACCAAGATGGAGAGAGACATCCTCGC
  TGACGTGAACCACCCGTTCGTGGTGAAGCTGCATTATGCCTTCCAGACCGAGGGCAAGCTCTATCTTATT
  CTGGACTTCCTGCGTGGTGGAGACCTGTTCACACGGCTCTCAAAGGAGGTCATGTTTACAGAGGAGGATG
  TGAAGTTTTACCTGGCTGAGCTGGCACTGGGCCTGGACCACCTGCACAGCTTGGGCATTATTTACAGAGA
  CCTCAAGCCTGAGAATATCCTTTTGGATGAGGAGGGCCACATCAAACTCACTGACTTTGGCCTGAGCAAG
  GAGGCCATAGACCATGAGAAGAAGGCTTACTCCTTCTGCGGGACAGTGGAGTACATGGCGCCCGAGGTTG
  TCAACCGCCAGGGTCACACCCACAGTGCAGACTGGTGGTCCTATGGGGTGCTGATGTTTGAGATGCTGAC
  GGGCTCCCTGCCCTTCCAGGGGAAGGACCGGAAGGAGACCATGACCTTGATTTTGAAGGCGAAGCTAGGC
  ATGCCCCAGTTTCTGAGCACGGAAGCCCAGAGCCTCCTGCGGGCCCTGTTCAAGAGGAATCCTGCCAATC
  GGCTTGGCTCGGGCCCTGATGGGGCAGAGGAAATTAAGAGACATATCTTCTACTCCACCATTGACTGGAA
  TAAGCTCTACCGGCGTGAGATCAAGCCCCCTTTCAAGCCGGCTGTGGCCCAGCCCGACGACACCTTCTAC
  TTTGATACCGAGTTCACGTCACGCACACCCAGGGATTCGCCAGGCATCCCCCCCAGTGCTGGTGCCCATC
  AGCTGTTCCGGGGCTTCAGCTTCGTGGCCACTGGTCTGATGGAGGACGACGGCAAGCCTCGGACCACTCA
  GGCCCCCCTACACTCGGTGGTACAGCAACTCCACGGGAAGAACTTGGTTTTCAGTGACGGCTACGTAGTA
  AAGGAGACGATCGGCGTGGGCTCCTACTCTGTGTGTAAGCGTTGTGTCCACAAGGCCACCAACATGGAGT
  ATGCTGTCAAGGTCATCGACAAGAGCAAAAGAGATCCCTCAGAAGAGATTGAGATTCTTCTGCGGTATGG
  CCAGCACCCCAACATCATCACCCTGAAAGATGTGTACGACGATGGTAAGCACGTGTACCTGGTGACAGAG
  CTGATGAGGGGCGGCGAGCTGCTGGATAAGATCCTACGGCAGAAGTTCTTCTCAGAGCGGGAGGCCAGCT
  TCGTCCTGCACACGATCAGCAAGACTGTGGAATACTTGCACTCTCAAGGGGTTGTCCACAGAGATCTCAA
  ACCCAGTAATATCCTCTATGTGGATGAGTCTGGGAACCCCGAGTGCCTGCGCATCTGCGACTTTGGCTTT
  GCCAAGCAGCTACGGGCCGAGAACGGACTCCTCATGACACCTTGCTACACAGCCAACTTTGTGGCACCTG
  AGGTGCTGAAGCGCCAGGGCTACGATGAAGGCTGTGACATATGGAGCCTGGGCATTCTGCTGTACACGAT
  GCTGGCAGGATACACTCCATTTGCCAATGGGCCCAGTGACACCCCAGAGGAGATCCTCACCCGGATCGGC
  AGCGGGAAGTTCACCCTCAGTGGGGGAAACTGGAACACGGTTTCAGAGACAGCCAAGGACTTGGTATCTA
  AGATGCTGCATGTGGACCCCCACCAGCGCCTCACAGCCAAGCAGGTCCTGCAGCACCCATGGATCACCCA
  GAAAGACAAGCTTCCCCAGAGCCAGTTGTCCCACCAAGACCTGCAGCTCGTGAAGGGAGCCATGGCAGCT
  ACATACTCTGCTCTCAATAGCTCCAAACCCACCCCTCAGCTCAAGCCAATTGAGTCGTCTATCCTGGCCC
  AGCGGCGGGTGAGGAAGCTGCCATCCACCACCCTGTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCTGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: BC094470
• Insert Size: 2208 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC094470, AAH94470
• RefSeq Size: 3097 bp
• RefSeq ORF: 2207 bp
• Locus ID: 20111
• Cytogenetics: 4 D2.3
• Gene Summary: Serine/threonine-protein kinase that acts downstream of ERK (MAPK1/ERK2 and MAPK3/ERK1) signaling and mediates mitogenic and stress-induced activation of the transcription factors CREB1, ETV1/ER81 and NR4A1/NUR77, regulates translation through RPS6 and EIF4B phosphorylation, and mediates cellular proliferation, survival, and differentiation by modulating mTOR signaling and repressing pro-apoptotic function of BAD and DAPK1. In fibroblast, is required for EGF-stimulated phosphorylation of CREB1, which results in the subsequent transcriptional activation of several immediate-early genes. In response to mitogenic stimulation (EGF and PMA), phosphorylates and activates NR4A1/NUR77 and ETV1/ER81 transcription factors and the cofactor CREBBP. Upon insulin-derived signal, acts indirectly on the transcription regulation of several genes by phosphorylating GSK3B at 'Ser-9' and inhibiting its activity. Phosphorylates RPS6 in response to serum or EGF via an mTOR-independent mechanism and promotes translation initiation by facilitating assembly of the pre-initiation complex. In response to insulin, phosphorylates EIF4B, enhancing EIF4B affinity for the EIF3 complex and stimulating cap-dependent translation. Is involved in the mTOR nutrient-sensing pathway by directly phosphorylating TSC2 at 'Ser-1798', which potently inhibits TSC2 ability to suppress mTOR signaling, and mediates phosphorylation of RPTOR, which regulates mTORC1 activity and may promote rapamycin-sensitive signaling independently of the PI3K/AKT pathway. Mediates cell survival by phosphorylating the pro-apoptotic proteins BAD and DAPK1 and suppressing their pro-apoptotic function. Promotes the survival of hepatic stellate cells by phosphorylating CEBPB in response to the hepatotoxin carbon tetrachloride (CCl4). Mediates induction of hepatocyte prolifration by TGFA through phosphorylation of CEBPB (PubMed:10635333). Is involved in cell cycle regulation by phosphorylating the CDK inhibitor CDKN1B, which promotes CDKN1B association with 14-3-3 proteins and prevents its translocation to the nucleus and inhibition of G1 progression (By similarity). Phosphorylates EPHA2 at 'Ser-897', the RPS6KA-EPHA2 signaling pathway controls cell migration (By similarity).[UniProtKB/Swiss-Prot Function]