Ints6 (BC058637) Mouse Untagged Clone

CAT#: MC220740

Ints6 (untagged) - Mouse integrator complex subunit 6 (cDNA clone MGC:61331 IMAGE:6400040), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Ints6
• Synonyms: HDB, DICE1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >BC058637
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCCCATCTTACTGTTCCTGATAGACACGTCTGCCTCTATGAACCAGCGCAGCCATCTGGGCACCACCT
  ACCTGGACACGGCCAAAGGCGCGGTAGAGACCTTCATGAAGCTCCGTGCCCGGGACCCTGCCAGCAGAGG
  AGACAGGTATATGCTGGTCACTTTCGAAGAGCCGCCCTATGCTATCAAGGCTGGATGGAAAGAAAACCAT
  GCAACGTTTATGAATGAACTGAAAAACCTTCAGGCAGAAGGACTTACGACTCTTGGCCAATCCCTAAGGA
  CAGCTTTTGATTTGTTAAATTTAAATAGATTAGTAACTGGCATAGACAACTATGGGCAGGGAAGAAACCC
  CTTTTTCTTGGAGCCAGCAATAATAATTACAATCACTGATGGGAGCAAGTTGACTACTACTAGTGGTGTC
  CAGGATGAGCTTCATTTACCTCTGAATTCGCCGTTGCCTGGAAGTGAATTGACCAAGGAACCTTTTCGTT
  GGGATCAGAGACTATTTGCTTTAGTGCTGCGGTTGCCTGGCACCATGTCGGTGGAATCAGAACAGTTGAC
  AGGTGTGCCTTTGGATGATTCTGCTATCACACCAATGTGTGAAGTGACAGGAGGCCGGTCATACTCTGTA
  TGTTCTCCAAGAATGCTTAATCAGTGTCTGGAATCCTTGGTGCAGAAAGTACAAAGTGGGGTGGTAATAA
  ACTTTGAAAAGGCAGGACCAGATCCTCCCCCTGCTGAAGCAGAGGGGCAGCCAGATATATCAAGACCTTT
  TGGATCTCAGCCTTGGCATAGCTGTCACAAGCTCATATATGTCAGACCAAATCCTAAAACTGGGGTTCCT
  ATAGGCCATTGGCCTGTTCCAGAGTCTTTTTGGCCAGATCAGAATTCTCCAACACTACCACCTCGTACAT
  CTCATCCTGTAGTGAAGTTTTCCTGTACAGACTGTGAGCCAATGGTTATTGACAAATTGCCTTTTGACAA
  ATATGAGTTGGAACCTTCACCACTGACTCAGTTTATTCTAGAAAGGAAATCTCCTCAAACGTGTTGGCAG
  GTGTATGTGAGCAATAGTGCAAAGTATAATGAACTTGGCCATCCCTTTGGTTACTTGAAAGCTAGTACAG
  CACTGACCTGTGTCAACTTATTTGTGATGCCTTACAATTACCCAGTCCTCCTTCCTCTATTAGATGACTT
  GTTCAAAGTGCACAAAGCAAAACCAACTCTGAAATGGAGACAGTCATTTGAAAGTTATTTGAAGACAATG
  CCTCCCTACTATCTTGGGCCCCTGAAGAAAGCTGTTAGAATGATGGGTGCACCTAATCTGATAGCAGACA
  GTATGGAGTATGGACTCAGTTACAGTGTCATTTCATACCTCAAAAAATTGAGTCAACAGGCCAAAATTGA
  ATCTGATAGAGTCATCGGATCCGTAGGCAAAAAAGTAGTACAGGAAACTGGAATTAAAGTCCGAAGCCGG
  TCACATGGGTTGTCAATGGCGCATAGGAAAGGTTTTCAAGTGCTGCAGGGTATTTCGGAGGATGTCCCCC
  ACAGGTTGCTAGACCTTAATATGAAGGAGTACACTGGGTTCCAGGTTGCTTTACTAAATAAGGATTTGAA
  GCCTCAGACCTTTAGAAACGCTTATGATATACCAAGACGGAACCTTCTGGATCATTTAACAAGAATGAGA
  TCGAACCTTTTAAAGAGCACCCGCAAATTTCTTAAAGGCCAGGATGAAGATCAAGTGCACAGTGTACCTA
  TAGCACAAATGGGGAATTACCAGGAATACCTCAAGCAAGTACCTTCTCCATTGAGAGAACTTGATCCTGA
  TCAGCCTCGAAGGTTGCATACATTTGGCAATCCCTTTAAACTGGATAAAAAGGGTATGATGATAGATGAA
  GCAGATGAATTTGTGGCTGGACCTCAGAATAAACACAAGCGGCCTGGAGAGCCAAGCATGCAAGGGATCC
  CAAAAAGACGCCGGTGTGCCTCTCCACTCTTGAGAGGCCGAAGGCAGAGCCCTGCTGTGAACAGTCACAT
  CGGAGGGAAAGGACCACCTGCACCCATGACGCAAGCACAGCCAGAAGCCACTAATGATTCAATAGTAGAT
  GATGTGGTTGAAAATCATGTTGCTGACCAACTTTCATCAGACATGACACCAAATGCTATGGATACTGAAT
  TTTTAACATCTCCCCCTAATCTTTTGGAACCATCAACCAATCATACAGAGGCCCTTGGTCATGAACATTT
  AGGAAACAATGACCTCACTGTTGGTGGGTTTTTAGAAAATCATGAGGAGCCAAGAAATAAAGAACAAAGT
  GCCGAAGAGAATATACCAGCATCTTCACTCAACAAAGGAAAGAAATTGATGCATTGTAGAAGCCATGAAG
  AGGTCAACACTGAACTAAAAGCACAAATAATGAAAGAGATCAGAAAGCCAGGAAGAAAATATGAAAGAAT
  CTTCACCTTACTGAAGCATGTGCAAGGCAGTTTACAAACAAGGCTAATATTCTTACAAAATGTCATTAAG
  GAAGCATCCAGGTTTAAGAAACGAATGCTAATAGAACAACTGGAGAACTTCTTGGATGAAATTCATCGAA
  GAGCCAATCAAATCAACCATATTAATAGCAATTAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCTGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: BC058637
• Insert Size: 2625 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC058637
• RefSeq Size: 4125 bp
• RefSeq ORF: 2624 bp
• Locus ID: 18130
• Cytogenetics: 14 D1
• Gene Summary: Component of the Integrator (INT) complex, a complex involved in the small nuclear RNAs (snRNA) U1 and U2 transcription and in their 3'-box-dependent processing. The Integrator complex is associated with the C-terminal domain (CTD) of RNA polymerase II largest subunit (POLR2A) and is recruited to the U1 and U2 snRNAs genes. Mediates recruitment of cytoplasmic dynein to the nuclear envelope, probably as component of the INT complex.[UniProtKB/Swiss-Prot Function]