Hgs (NM_001159328) Mouse Untagged Clone

CAT#: MC220726

Hgs (untagged) - Mouse HGF-regulated tyrosine kinase substrate (Hgs), transcript variant 1, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Hgs
• Synonyms: Hgr; Hrs; tn; ZFYVE8
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC220726 representing NM_001159328
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGGCGAGGCAGCGGCACCTTCGAGCGTCTCCTAGACAAAGCCACCAGCCAGCTTCTGTTGGAGACAG
  ACTGGGAGTCCATTCTACAGATCTGCGACCTGATCCGTCAGGGGGACACACAAGCAAAATATGCTGTAAA
  CTCCATCAAGAAGAAGGTTAATGATAAGAACCCACACGTGGCTTTGTATGCTCTGGAGGTGATGGAGTCT
  GTGGTAAAGAACTGTGGCCAGACAGTCCATGATGAAGTGGCCAACAAACAGACCATGGAAGAACTGAAGG
  AGCTGCTGAAGAGGCAAGTGGAAGTTAATGTTCGGAACAAGATCTTGTACCTGATCCAGGCCTGGGCACA
  TGCCTTCCGGAACGAACCCAAGTACAAGGTGGTCCAGGACACATACCAGATCATGAAGGTAGAAGGACAT
  GTCTTCCCTGAATTTAAGGAAAGTGATGCCATGTTCGCTGCTGAAAGAGCCCCTGACTGGGTGGATGCTG
  AGGAATGCCATCGGTGCAGAGTACAGTTTGGGGTGGTGACCCGCAAGCATCACTGCCGAGCATGTGGGCA
  GATCTTCTGTGGCAAGTGCTCCTCCAAGTACTCCACCATCCCCAAGTTCGGCATTGAGAAGGAGGTGCGC
  GTGTGTGAGCCCTGCTATGAGCAGCTGAACAAGAAGGCAGAAGGGAAGGCTTCCTCTACCACTGAGCTGC
  CCCCAGAGTACCTGACCAGCCCCCTGTCACAGCAGTCTCAGCTGCCCCCAAAGCGGGATGAGACAGCCCT
  GCAGGAAGAGGAGGAGCTACAGCTGGCTCTGGCCCTATCACAGTCAGAGGCTGAGGAGAAAGAGAGGATG
  AGACAGAAAACAACATATACAGCACATCCAAAGGCAGAGCCCACGCCCTTGGCTTCCTCTGCGCCCCCAG
  CTGGCAGCCTGTATTCCTCGCCTGTGAACTCATCAGCACCTCTGGCTGAGGACATCGACCCTGAGCTTGC
  AAGATACCTCAACCGGAACTACTGGGAGAAGAAACAGGAGGAAGCACGGAAGAGCCCCACACCATCTGCA
  CCTGTGCCCCTGACAGAGCCTGCTGCCCAGCCTGGAGAAGGACATACAGCCCCCAACAGCATGGCAGAGG
  CTCCTCTTCCAGAGACAGACTCTCAGCCCATAACTCCCTGCAGCGGCCCCTTTAGTGAGCAGTACCAGAA
  TGGGGAGTCGGAGGAGAGCCATGAGCAGTTCCTCAAGGCCCTGCAGAATGCCGTGAGCACTTTTGTCAAC
  CGCATGAAGAGCAACCACATGCGGGGACGCAGCATCACCAACGACTCGGCTGTGCTGTCACTCTTCCAGT
  CCATCAACACCATGCACCCGCAGCTGCTCGAGCTGCTCAACCAGCTGGATGAGCGCAGGCTGTACTACGA
  GGGCCTTCAGGACAAGCTGGCACAGATACGTGACGCCCGAGGGGCTCTGAGCGCCCTGCGTGAAGAACAC
  AGGGAGAAGCTGCGCCGGGCAGCTGAGGAGGCTGAGCGTCAACGCCAGATCCAGCTGGCACAGAAACTGG
  AGATCATGAGACAGAAGAAGCAGGAGTACCTGGAGGTGCAGAGACAGCTAGCTATCCAGCGCCTGCAGGA
  ACAGGAGAAGGAACGGCAGATGCGTCTGGAGCAACAGAAGCAGACTGTCCAGATGCGCGCCCAGATGCCT
  GCCTTTCCCTTGCCTTATGCCCAGCTCCAGGCTATGCCCACGGCTGGGGGTGTACTCTACCAGCCCTCAG
  GCCCAACCAGCTTCCCTGCCACCTTCAGCCCAGCAGGCTCAGTAGAGGGCTCTCCGATGCATGGTGTGTA
  TATGAGCCAGCCAGCCCCAGCCACTGGCCCCTACCCCAGCATGCCTGGCACAACAGCAGATCCCAGCATG
  GTCAGCGCCTACATGTACCCAACAGGTGCCCCTGGGGCACAGGCAGCCCCTCAGGCCCAGGCCGGGCCCA
  CCACCAGTCCTGCCTACTCCTCCTACCAGCCCACACCAACCCCAGGCTACCAGAGCGTGGCTTCTCAGGC
  CCCACAGAGCCTCCCAGCCATCTCACAGCCTCCACAGACCAGCAACATAGGCTACATGGGGAGCCAGCCA
  ATGTCCATGGGCTACCAGCCGTACAATATGCAGAATCTCATGACCGCCCTTCCCGGGCAGGATGCGTCTC
  TGCCAGCCCAGCAGCCCTACATCCCAGGGCAGCAGCCCCTGTACCAGCAGATGGCCCCCAGCACCGGCCC
  TCCCCAGCAGCAACCCCCTGTGGCCCAGCCAGCGCCTACACAGGGACCGCCAGCACAGGGCAGTGAGGCC
  CAGCTCATCTCCTTTGACTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001159328
• Insert Size: 2331 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001159328.1, NP_001152800.1
• RefSeq Size: 2908 bp
• RefSeq ORF: 2331 bp
• Locus ID: 15239
• UniProt ID: Q99LI8
• Cytogenetics: 11 84.16 cM
• Gene Summary: Involved in intracellular signal transduction mediated by cytokines and growth factors. When associated with STAM, it suppresses DNA signaling upon stimulation by IL-2 and GM-CSF. Could be a direct effector of PI3-kinase in vesicular pathway via early endosomes and may regulate trafficking to early and late endosomes by recruiting clathrin. May concentrate ubiquitinated receptors within clathrin-coated regions. Involved in down-regulation of receptor tyrosine kinase via multivesicular body (MVBs) when complexed with STAM (ESCRT-0 complex). The ESCRT-0 complex binds ubiquitin and acts as sorting machinery that recognizes ubiquitinated receptors and transfers them to further sequential lysosomal sorting/trafficking processes. May contribute to the efficient recruitment of SMADs to the activin receptor complex. Involved in receptor recycling via its association with the CART complex, a multiprotein complex required for efficient transferrin receptor recycling but not for EGFR degradation.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1).