Prom1 (NM_001163585) Mouse Untagged Clone

CAT#: MC220647

Prom1 (untagged) - Mouse prominin 1 (Prom1), transcript variant 8, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Prom1
• Synonyms: 4932416E19Rik; AC133; CD133; Prom; Prom-1; Proml1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC220647 representing NM_001163585
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCTCTCGTCTTCAGTGCCCTGCTGTTACTGGGGCTGTGTGGAAAGATCTCTTCAGAAGGTCAGCCTG
  CATTCCATAACACTCCTGGGGCTATGAATTATGAATTGCCTACCACCAAATATGAGACCCAAGATACCTT
  CAATGCTGGGATTGTTGGCCCTCTCTACAAAATGGTGCACATCTTCCTCAACGTGGTCCAGCCGAATGAC
  TTCCCTCTAGATTTGATCAAAAAACTCATACAGAACAAGAACTTTGACATCTCAGTTGATTCCAAGGAGC
  CAGAAATCATAGTCTTGGCTCTGAAGATTGCCCTCTATGAGATCGGAGTCCTTATCTGCGCCATCCTGGG
  ACTGCTGTTCATTATCCTCATGCCTCTGGTGGGCTGCTTCTTTTGTATGTGCCGTTGCTGCAACAAATGC
  GGCGGAGAGATGCACCAGCGGCAGAAGCAGAATGCGCCATGCAGGAGGAAGTGCTTGGGCCTCTCCCTCC
  TGGTGATTTGTCTGCTCATGAGCCTTGGCATTATATATGGCTTTGTGGCTAACCAGCAGACCAGGACTCG
  GATCAAAGGGACCCAGAAACTGGCAAAGAGCAATTTCAGAGACTTTCAAACACTCCTGACTGAAACACCA
  AAGCAAATTGACTATGTAGTGGAGCAGTACACCAACACCAAGAACAAGGCATTCTCAGACCTGGATGGCA
  TCGGCTCCGTGCTGGGAGGCAGAATAAAGGACCAACTAAAACCCAAAGTAACTCCTGTCCTCGAAGAGAT
  TAAGGCCATGGCGACAGCCATCAAACAGACCAAGGATGCCCTGCAGAACATGAGCAGCAGCCTGAAAAGT
  CTCCAAGATGCAGCCACCCAGCTCAATACCAACCTGAGCTCTGTGAGAAACAGCATCGAGAATTCGCTCA
  GCAGCAGTGACTGTACCTCAGATCCAGCCAGCAAGATCTGCGATAGCATCAGACCAAGCCTAAGCAGTCT
  GGGGAGCAGCCTCAATTCAAGTCAGCTCCCATCAGTGGATAGAGAACTCAACACTGTTACTGAAGTCGAC
  AAAACTGATCTGGAGAGCCTCGTCAAAAGGGGGTATACGACAATTGATGAAATACCCAATACAATACAAA
  ACCAAACTGTGGATGTCATCAAAGACGTCAAAAATACCTTGGACTCCATTAGCTCCAACATTAAGGACAT
  GAGCCAAAGTATTCCTATTGAGGATATGCTGTTACAGGTCTCCCATTACCTTAATAACAGCAACAGATAC
  TTAAACCAGGAGCTGCCCAAGCTGGAAGAATATGACTCGTACTGGTGGCTGGGTGGCTTGATTGTCTGCT
  TTCTGCTGACTCTCATTGTGACCTTCTTTTTCCTGGGCTTGCTGTGTGGTGTGTTTGGCTATGACAAGCA
  TGCCACCCCAACTAGAAGAGGCTGTGTGTCCAACACTGGAGGCATCTTCCTCATGGCTGGGGTTGGATTC
  GGCTTCCTTTTTTGCTGGATATTGATGATCCTTGTGGTTCTTACGTTTGTTGTTGGTGCAAATGTGGAAA
  AGTTGCTCTGCGAACCTTATGAAAACAAGAAATTATTACAGGTTTTGGACACTCCCTATCTGCTCAAGGA
  ACAATGGCAATTTTATCTTTCTGGCATGCTATTCAATAACCCAGACATTAACATGACCTTTGAGCAAGTC
  TACAGGGATTGCAAAAGAGGTCGAGGTATATATGCTGCTTTTCAGCTTGAGAATGTCGTCAACGTCAGTG
  ATCATTTCAACATTGACCAGATTTCTGAAAACATAAATACGGAGTTGGAAAACCTGAATGTGAACATTGA
  TAGCATTGAACTGTTGGATAACACAGGAAGGAAGAGCCTCGAGGACTTTGCACATTCTGGGATAGATACA
  ATCGATTATTCCACATACTTGAAGGAGACTGAGAAATCCCCTACTGAAGTGAATCTGCTGACATTTGCCT
  CTACCCTGGAAGCAAAAGCAAACCAGTTGCCTGAAGGAAAGCTGAAACAGGCCTTCTTACTGGATGTACA
  GAATATAAGAGCCATCCACCAGCATCTCCTCCCTCCTGTGCAGCAATCACTGAAATTTGTGAGGGTGAGG
  AATACGTTAAGACAAAGTGTCTGGACCCTCCAGCAAACAAGCAACAAGTTGCCGGAGAAAGTGAAGAAGA
  TCCTTGCCTCTTTGGACTCTGTTCAGCATTTCCTCACCAATAACGTTTCCCTCATCGTTATCGGGGAAAC
  GAAGAAGTTTGGGAAAACAATACTAGGCTACTTTGAACATTATCTGCACTGGGTCTTTTATGCCATCACA
  GAGAAGATGACATCCTGCAAACCCATGGCCACCGCGATGGACTCTGCTGTTAATGGCATTCTGTGTGGCT
  ATGTTGCGGACCCTCTGAATTTGTTCTGGTTCGGCATAGGGAAAGCCACGGTGCTCTTACTTCCGGCTGT
  AATCATTGCTATCAAGCTGGCCAAGTACTATCGCAGGATGGATTCAGAGGATGTATACGACGACCCGTCT
  CGATACTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001163585
• Insert Size: 2529 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001163585.1, NP_001157057.1
• RefSeq Size: 3602 bp
• RefSeq ORF: 2529 bp
• Locus ID: 19126
• Cytogenetics: 5 B3
• Gene Summary: May play a role in cell differentiation, proliferation and apoptosis. Binds cholesterol in cholesterol-containing plasma membrane microdomains and may play a role in the organization of the apical plasma membrane in epithelial cells. During early retinal development acts as a key regulator of disk morphogenesis (PubMed:19228982). Involved in regulation of MAPK and Akt signaling pathways. In neuroblastoma cells suppresses cell differentiation such as neurite outgrowth in a RET-dependent manner.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (8) lacks an alternative in-frame exon in the 5' coding region and has multiple differences in the presence and absence of exons at its 3' end, compared to variant 2. The resulting isoform (s8) lacks a 9 aa segment near the N-terminus and has a shorter C-terminus, compared to isoform s2. The full structure of the 5' UTR for this variant has not been determined.