Nolc1 (NM_053086) Mouse Untagged Clone

CAT#: MC220593

Nolc1 (untagged) - Mouse nucleolar and coiled-body phosphoprotein 1 (Nolc1), transcript variant 1, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Nolc1
• Synonyms: NOPP130; NOPP140; P130
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC220593 representing NM_053086
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGGATACCGGCTTGCGCCGCGTGGTTCCCAGCGACCTTTATCCCCTTGTGCTCAGATTTCTGCGGG
  ATAGCCAACTCTCGGAGGTGGCCAGTAAATTTGCAAAAGCGACCGGCGCTACACAGCAGGACGCCAATGC
  CTCGTCCCTCTTGGACATCTATAGCTTCTGGCTCAACAGGTCCACCAAAGCCCCAAAGGTGAAGTTACAG
  TCAAATGGACCAGTGACCAAGAAGGCTAAGAAAGAGACTTCATCCAGTGACAGCAGTGAGGACAGCAGTG
  AGGACGAGGACAAAAAAGCCCAGGGACTTCCCACACAGAAGGCTGCCGCACAGGTCAAGCGAGCCAGTGT
  GCCTCAGCATGCTGGAAAGGCAGCAGCCAAAGCTTCAGAGAGCAGCAGTAGTGAAGAATCCAGTGAGGAA
  GAGGAAGAGGACAAAAAGAAAAAGCCTGTCCAGAAGGCAGCTAAGCCCCAAGCCAAGGCAGTCAGACCTC
  CTGCGAAGAAGGCAGAGAGCTCTGAGTCGGACTCAGACTCGGATTCGGACTCCAGCTCAGAGGAAGAAAC
  ACCACAGACCCAGAAGCCAAAGGCAGCTGTGGCAGCAAAAGCTCAGACTAAAGCCGAAGCCAAACCAGGT
  ACACCAGCGAAAGCACAGCCTAAGGTAGCCAATGGCAAAGCAGCCGCCAGCAGCAGCAGCAGCAGCAGCA
  GCGATGACTCAGAGGAAGAGAAGAAGGCAGCTGCACCTCCCAAGAAGACTGTACCAAAAAAGCAAGTCGT
  GGCCAAGGCCCCAGTGAAAGTAGCTGCCGCCCCCACCCAGAAGAGCTCCAGCAGTGAGGATTCTTCCAGT
  GAAGAGGAGGAGGGACAGAGACAACCCATGAAGAAAAAAGCAGGTCCCTACAGTTCAGTTCCACCACCCT
  CTGTTCCTTTACCAAAGAAGTCCCCGGGAACCCAGGCTCCAAAGAAAGCTGCTGCGCAGACACAGCCTGC
  AGACAGCAGTGACGACAGCAGTGACGATTCTGATTCAAGTTCTGAGGAAGAGAAAAAACCTCCAGCTAAG
  ACGGTCGTCTCCAAGACACCCGCCAAAGCAGCTCCAGTGAAGAAGAAAGCAGAAAGCTCTTCAGACAGCT
  CGGATTCTGACAGTTCTGAGGATGAAGCTCCTGCCAAGCCAGTCAGTACAACCAAGAGTCCCAAGCCAGC
  TGTCACTCCGAAGCCATCTGCAGCAAAGGCAGTGACAACTCCTAAGCAACCTGCAGGCAGTAACCAGAAA
  CCTCAGAGCAGGAAGGCTGACAGCAGCTCCAGCGAGGAGGAAAGCAGCTCCAGCGAGGAGGAGGAGGCCT
  CCAAGAAAAGTGCCACAACCCCCAAGGCCAAGGTGACTGCTAAAGCAGCACCCGCCAAACAGGCCCCTCA
  GGCTGCTGGGGACAGCAGCTCTGACTCAGATAGTTCCAGCAGTGAAGAGGAGGAGAAGACTCCTAAGCCC
  CCAGCTAAGAAGAAGGCAGCAGGTGGAGCCGTTTCTACACCAGCCCCTGGGAAGAAAGCAGAGGCCAAGA
  GCAGCAGCAGCAGCAGCAGCAGCAGCTCCGAAGATTCCAGTGAAGAGGAGAAAAAAAAGAAGCCCAAAGC
  TACTACCCCTAAAATACAGGCAAGCAAGGCCAATGGCACTCCAGCTTCTCTGAATGGAAAAGCAGCCAAG
  GAAAGTGAGGAGGAAGAGGAGGAGGAAGAAACAGAAGAGAAGAAAAAGGCAGCTGGGACCAAGCCAGGTT
  CAGGCAAAAAACGGAAGCAGAATGAGACCGCAGATGAAGCAACAACTCCTCAAGCTAAGAAAGTTAAGCT
  CGAGACCCCCAATACGTTTCCAAAAAGGAAGAAGGGAGAAAGAAGGGCGTCTTCCCCTTTCCGAAGGGTC
  AGGGAGGAGGAGATTGAGGTGGACTCTCGAGTGGCGGACAATTCCTTTGATGCCAAGCGAGGTGCAGCTG
  GAGACTGGGGGGAGCGAGCCAATCAGGTTCTGAAGTTCACCAAAGGCAAGTCCTTCCGGCATGAAAAAAC
  GAAGAAGAAGCGAGGCAGCTACCGGGGAGGCTCCATCTCTGTCCAGGTCAATTCCGTCAAATTCGACAGC
  GAGTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_053086
• Insert Size: 2106 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_053086.3, NP_444316.2
• RefSeq Size: 3662 bp
• RefSeq ORF: 2106 bp
• Locus ID: 70769
• UniProt ID: E9Q5C9
• Cytogenetics: 19 C3
• Gene Summary: Nucleolar protein that acts as a regulator of RNA polymerase I by connecting RNA polymerase I with enzymes responsible for ribosomal processing and modification (By similarity). Required for neural crest specification: following monoubiquitination by the BCR(KBTBD8) complex, associates with TCOF1 and acts as a platform to connect RNA polymerase I with enzymes responsible for ribosomal processing and modification, leading to remodel the translational program of differentiating cells in favor of neural crest specification (By similarity). Involved in nucleologenesis, possibly by playing a role in the maintenance of the fundamental structure of the fibrillar center and dense fibrillar component in the nucleolus (PubMed:11424213). It has intrinsic GTPase and ATPase activities (By similarity).[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (1) uses an alternate in-frame splice site in the central coding region, compared to variant 3. The encoded isoform (A) is shorter, compared to isoform C.