Trdn (NM_029726) Mouse Untagged Clone

CAT#: MC220518

Trdn (untagged) - Mouse triadin (Trdn), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Trdn
• Synonyms: 2310045H21Rik; EG432451; TDN
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC220518 representing NM_029726
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGACTGAGATCACTGCTGAAGGAAATGCATCGACAACCACAACGGTGATAGACAACAAAAATGGATGTA
  TTCCTAAATCCCCTGGAAAGGTGCTGAAGAGGTCTGTCACCGAAGACATTGTGACAACATTCAGCTCCCC
  TGCAGCCTGGCTTCTTGTCATCGCTCTGATTATCACATGGTCAGCTGTTGCTATCGTGATGTTTGATTTA
  GTGGATTATAAAAACTTTTCAGCAAGCTCCATTGCCAAGATTGGCTCAGATCCTCTAAAACTAGTGAATG
  ATGCTGTGGAGGAGACAACAGACTGGATCTATGGCTTCTTCTCTTTGCTATCTGACATCATCTCATCTGA
  AGGTGACGAAGATGATGAGGATGCAGATGAAGACATTGATAAAGGAGAAATAGAAGAACCTCCCTTAAAA
  AGAAAAGAAATACACCAAGAAAAGGCTGAAAAAGAGGAGAAACCTGAGAAGAAAATACAAACTAAAGCTT
  CACACAGAGAAAGGGAGAAAGGAAAAGAAAAATTAAAAGGAGAAAAACCTGAGAAGACAGCAACTCACAA
  AGAGAAACTTGAGAAAAAAGAAAGACCAGAGACGAAGATGATGGCAAAAGAGGACAAGAAAATTAAGACT
  AAGGAAAAGACTGAAGAAAAGGCTAAGAAGGAAATGAAAGTTGGAAAACAGGAGAAAGTGAAACCAACAG
  CTGCAAAAGCCAAAGAAACTCCGAAAACACCACCAAAGGCCAGAAAGAAGGATGACAAAGAGATGCCAGC
  TGTGCATGAGCAGAAAGATCAGTATGCATTCTGTCGATATATGATTGACATGTTTGTCCATGGGGATTTA
  AAACCAGGACAAAGCCCAGTTATGCCACCACCATCATTGACACCTTCCAAACCTGCTTTGTCAACAACGG
  CCCTTGAAGAAAAAGAAAAAGAAGAGAAGAAGAAAATGGAGAAGAAAGATACTTCTGATACAAAAAAGAA
  AGAAAAAGAAGTCAAAAAGAAAAGTGAAGAAACTACCATTGATGGGAAAGGCAAAGAACCAGGCAAACCT
  CCTGAAACCAAACAAATGACCGCCAAACTCACAACACAAGCTGCAGCTAGAAAGGATGAGAAGAAAGAAG
  AATCAAAGAAAATGAGAAAACCTACAGAAGAACAACCCAAGGGAAAAAAACAGGAAAAGAAAGAAAAGCA
  CATAGAACCAGCAAAGACACCAAAAAAGGAACATCCAGGTCCAAGTGAAAAACTCAAAAAAGCAAAAGCT
  GAACAAGCCAAGGAAGAGATTGCTGCTGCCTCAACAAAAAAGGCCCTGCATGGAAAGAAAGAAGAGAAAG
  CCAAGACAGTCGAGCAAGAAGTGAAAAAAGAAAAATCTGGGAAGAGTTCTTCAGATTTGAAAGATAAAGA
  AGTGAAAAAAGAAAAGTCTGGGAAGAGTTCTTCAGATTTGAAAGATAAAGAGCCACAACTAAAAAACGAA
  GAGAAGTCAAAGCCACAAGTGAAAAAGGAAGCAAAACTAGCATCATCTGACAAAGGCCAAACACGCAAAC
  AGAACATCACCAGACCAGAGCAAGTTATTCCTCATGTTAAACCAGAAAAAGCTGAACACCAAGAAAAAGG
  CCACCCATCTATAAAAAAAGATAAGCCTAAACCATCTTCAAAGGGAGCACCAGAAGTTCCAGACTCAGGG
  AAGAAGAAGATTGAAAAATCTGAAAAGGAAAGTAAAGTTCCAACAAGAGAAGAAAATCTTCAGGTGTACA
  ATGTGACTAAAGCAGAAAAACCTGGAAAAATACCAAAAGATTCCAAAGAAGCACCAGCTTCAAAGAAAGA
  TAAAGAAGATTCCAAAGAAGCACCAACTTCAAAGAAAGATAAAGAAGACTCCAAAGATGTCCCACATTCA
  AAGAAAGATAAAGAAGTAACTGATGATGTATCTTCACCAAAGAAGCAAACACGTCCCATCAGCTTCTTCC
  AATGTGTGTACTTGAATGGGTACAATGGCTATGGGTTTCAGTTTCCTGTCACTCCTGTCCAACAACCTGG
  AGAGAACCCTGGCAAAACAAACTCTCCAGGACAGAAGCAACAAGAACAATAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_029726
• Insert Size: 2082 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_029726.2, NP_084002.2
• RefSeq Size: 4514 bp
• RefSeq ORF: 2082 bp
• Locus ID: 76757
• UniProt ID: E9Q9K5
• Cytogenetics: 10 A4
• Gene Summary: Contributes to the regulation of lumenal Ca2+ release via the sarcoplasmic reticulum calcium release channels RYR1 and RYR2, a key step in triggering skeletal and heart muscle contraction. Required for normal organization of the triad junction, where T-tubules and the sarcoplasmic reticulum terminal cisternae are in close contact. Required for normal skeletal muscle strength (PubMed:19843516). Plays a role in excitation-contraction coupling in the heart and in regulating the rate of heart beats.[UniProtKB/Swiss-Prot Function]