Hjurp (NM_198652) Mouse Untagged Clone

CAT#: MC220297

Hjurp (untagged) - Mouse Holliday junction recognition protein (Hjurp), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Hjurp
• Synonyms: 6430706D22Rik; A730008H23Rik; C330011F01Rik; Gm975; Mfleg1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC220297 representing NM_198652
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGTCTATGGGTCGGCAGGACCGCAGGCTGCATCAGCAGCTTAAGGAAAGCAGTAGCCGCTTCCAGA
  CGTTAATGAAGCGGCTGATAGCGAAGTACAACCAGCCCTTTGAGGACGACCCGTTGGTGGAGATGAGGAC
  GTTAACCTACGAGACGCCCCAGGGGTTGAGAGTTTGGGGAGGAAAGCTGATGAAGAAGGAAGACAAGGAA
  TACACTCAGGTGATTGACAGATTAAATGGGCAAGCTCCAGAAGGTGATTCAGAGAGCAGCGGTGCGGATA
  CGAGCTTAGAGGAGAACTGGCCATCTTGCAGCTCAGCGATGAGAGAAGCCTCTGGTGATCCTAGGCAGCG
  TCAGCCTGCAGTGCCCGGAAACACCTTGGAAACTGATTTAAGAAGGAAGTACTTGACCCAGGTGGATATA
  TTGCCTCAAGATGAAGAGTATTTTAAGAACGCAGAGAAGAGAGGTGGAAAAGACACAGTCATGACTTGGG
  TTCCTTCCGTGACCTCATCTGTCACACCAGCCTCTGGATGCCAGGATGCTATCTCTGCAAAGAGCTCTGG
  TGGTCCTGAAGTATCTGCTTTGTCCTCTAGAGGCCAGGGTCCCTCATACCCTTGCCCAGCTGATATGGCC
  ATTGTGGCTAGAAGTGATGGCCTCTCCTTACTTGGGACCAGCAGCAACAGCGTAAGCAGCCAGTCCTTTG
  AGGTTGATGACCTTTGCAATGTGACAATCAGCGATTTGTATGAAGGCATGATGCATTCCATGAGTAGGCT
  GCTGCGCTCAAAGCCATCCTGCATCATCTCCACCAAGACCTACATCAACCAGAGCTGGAAGCTCCGGAGG
  AGACCCTCACGCAAGCAGGGGCTGCACAAGAACAGGACACACTGCCCCCGGAGCAAGCCCTCCCAGAGGA
  GTGCCAGGAAGGGGCCTGCGTCCTGCTCAGAGCCCGGGAAAGAGGCCGGAATATTAAGAGACTACGGGAA
  TTTACTGCACGTTGCTCCCCACAAGACAGGCTTAGAACTGAAAAGCGTTTCTCTTGAAGGAAGCAAACGC
  CAAGTCCATAAATCCAGTCCAGCTTGGAAGGAGCTCCAGATGATGCCGCAGAAGGACTTGGACTTAAACC
  GTGAGCGAGAAAATAGGGTGATGACATTACAGTGGCTAATTTCACCCGTCAAGGTGGTTCCCAGACCAAG
  GATGCTTCCAAGTCAAGTAGAGAAGTGGTATAGAGAGATTAAAATCAAATTTGACAAGCTTCATCAGGAA
  TATTGCCTGTCTTCTGGGAAACAGCCTCGCCTGACTGACCCCACAGAGTCTTGGGCTGTCGATGTGTACA
  GAAGTGGTTCTAAGAGCCCAGGCAGCCGTCAGGATGTAGAAACCTGCAGGCCCAGTTCACCTTTCGGCAG
  AGAAAAGACAGAAAGGCCAGGTGAAGCTTTAGAAGACCTGAGGGGAAATGGAAAGTCTGTCAAAACAAAG
  AGCTGTCTTCTAAGGAGCTGTCCCTCTCCAGAGGGCAGCCCGTCACGGAGCCCCAGCCATTCTCAGCTGA
  GCTCTGGCCTTCAAGAGCACAATTCTGAACCTACTGGTAAGGCAGTGTGGCCCAGTACAGCTATTTCAGC
  ACCAAGCATAGGCTCTCCAGGCTGTGGGAAGGATAACTATGATGAGCTTAAAAAAGAATTCAACAGACTT
  TATCAGAAGTATTGTCTGTCACCGCAGCGGGCGAAGGTGACTTCGTGTGGCAGAGTATCGCCAATGAAAG
  CTGCTGCAGCTCTTCCCTGTCAATCAGAGCACTTGAAGAGACTAAATCCAGACTCCCCACAACAGAGTTC
  CCAGAAACGGTCAATCTCTCCTGGCTGTCACAGAAGAGTCCTGCAGGATTCAACTGCACAGACTGCTAGT
  ACACTTGTGAGAGACTCTTGGCTGCCAACAAAGAGGTGCAAGTTGTCATACCCGGTAGCTTGTGCTCACC
  AGGCCAAGTTTCACGATACCTCAGGAGCATCAGGCTGGCCCTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_198652
• Insert Size: 2004 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_198652.2, NP_941054.2
• RefSeq Size: 8449 bp
• RefSeq ORF: 2004 bp
• Locus ID: 381280
• UniProt ID: Q6PG16
• Cytogenetics: 1 D
• Gene Summary: Centromeric protein that plays a central role in the incorporation and maintenance of histone H3-like variant CENPA at centromeres. Acts as a specific chaperone for CENPA and is required for the incorporation of newly synthesized CENPA molecules into nucleosomes at replicated centromeres. Prevents CENPA-H4 tetramerization and prevents premature DNA binding by the CENPA-H4 tetramer. Directly binds Holliday junctions.[UniProtKB/Swiss-Prot Function]