Rapgefl1 (NM_001080925) Mouse Untagged Clone

CAT#: MC220289

Rapgefl1 (untagged) - Mouse Rap guanine nucleotide exchange factor (GEF)-like 1 (Rapgefl1), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Rapgefl1
• Synonyms: B230105J10
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC220289 representing NM_001080925
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAAGCCACTGGAGAAATTTTTGAAGAAGCAGACGTCTCAGCTGGCGGGGCGAGCGGTGACTGGAGGTC
  CCGGCGGGGGTCCGGGTTGCTGCGGAGGGCCTGGAGGGGGTGGGGGCCCTGGTGGGGGCGGCGGTCCGGC
  CGGGGGACTGCGGCCACTGCAGCGGCGTCAGAGCGTGTCTCGCCTGCTGCTCCCAGCTTTCCTCCGAGAG
  CCCCCAACCGAGCCCGGGCTGGAGCCGCCCGTTGAGGAGGAAGGGGGAGAGCCATTAGGGGTCTCTGAGG
  AGCCGGGCAGTGGGGGGCCCTGTTGGCTGCAGCTAGAGGAGGTTCCCGGGCCTGGGCCGATAGGATCTGG
  GGTGCCCCTGCGCTCTCCTTCCTCGTACTCCTCAGATGAGCTATCCCCCGGCGAACCCCTGGCTTCACCA
  CCCTGGGCCCCCCTGGGCGCCCCCGAGCGGCCAGAGCATCTTCTGAACCGGGTTCTGGAGCGCCTGGCTG
  GAGGGACCACCAGGGACAGCAGCGCCTCAGATATTCTTCTTGACGACATTGTTCTTACCCATTCCCTCTT
  CCTGCCAACAGAAAAGTTTCTACAAGAGCTACACCAGTACTTTGTTCAGTCAAGGAATGTGGAGGGTCCA
  GAGGGGCTGGGTCGGAAGCAAGCCTGCCTTGCTCTCCTCCTCCATTTCCTGGATACCTACCAAGGACTTC
  TGCAAGAGGAAGAAGGGGCCGGCCACATTATCAAGGAACTGTATCTACTAATTATGAAGGATGAGTCTCT
  TTACCAGGACCTCCGAGAGGACACACTGAGGCTACACCAGCTGGTGGAGACAGTGGAGCTAAAGATTCCA
  GAAGAGAGCCAGCCGCCCAGCAAGCAGGTTAAGCCGCTCTTCCGACACTTCCGCCGGATAGATTCCTGTC
  TGCAGACCCGAGTGGCATTCAGGGGCTCCGATGAGATTTTCTGCCGAGTCTACATGCCTGACCACTCTTA
  CGTGACCATACGCAGTCGCCTCTCGGCATCAGTGCAAGACATTCTGGGCTCTGTGACAGAGAAGCTGCAG
  TACTCGGAGGAGCCTGCGGAGCGCGAGGATGCCCTCATCCTTGTAGCTGTTGCTTCCTCTGGAGAGAAGG
  TCCTCCTCCAGCCAACGGAGGACTGCGTTTTCACCACGCTGGGTATCAACAGCCACCTATTTGCCTGTAC
  CCGGGACAGCTACGAGGCCCTAGTACCCCTTCCGGAGGAGGTCCAGGTCTCCCCAGGAGACACAGAGATC
  CACCGGGTGGAGCCTGAAGATGTGGCCAATCACCTGACTGCTTTCCACTGGGAGCTCTTCCGATGTGTGC
  ACGAGTTGGAGTTCGTGGACTACGTATTCCACGGGGAGCGTGGCCGCCGAGAGACAGCCAACCTGGAGTT
  GCTGCTGCAGCGTTGCAGCGAGGTCACTCACTGGGTGGCCACTGAGGTGCTGCTCTGTGAGGCCCCGGGC
  AAGCGTGTACAGCTGCTTAAGAAGTTCATCAAGATCGCAGCCATCTGCAAGCAGAACCAGGACCTGCTGT
  CCTTCTATGCTGTGGTCATGGGGCTGGACAATGCGGCTGTCAGCCGCCTGCGGCTCACCTGGGAGAAGCT
  GCCAGGGAAATTCAAGAACCTGTTCCGTAAATTTGAGGGCTTGACGGACCCCTGCCGGAACCATAAAAGC
  TACAGAGAAGTCATCTCCAAAATGAAGCCCCCTCTGATTCCGTTCGTGCCCCTGATCCTCAAAGACCTGA
  CCTTCCTACATGAGGGGAGCAAGACCCTCGTAGATGGTCTGGTGAATATTGAGAAGCTGCATTCAGTGGC
  CGAGAAAGTGAGGACAATCCGAAAATACCGGAGCCGGCCCCTCTGCCTGGACATGGAGGCATCCCCACAT
  CACCTGCAGACCAAGGCGTACGTGCGCCAGTTCCAGGTCATCGACAACCAGAACCACCTCTTTGAGCTCT
  CCTACAAGTTGGAGGCTAATAGTCAGTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001080925
• Insert Size: 1989 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001080925.1, NP_001074394.1
• RefSeq Size: 4163 bp
• RefSeq ORF: 1989 bp
• Locus ID: 268480
• UniProt ID: Q68EF8
• Cytogenetics: 11 D
• Gene Summary: Probable guanine nucleotide exchange factor (GEF).[UniProtKB/Swiss-Prot Function]