Man1b1 (NM_001029983) Mouse Untagged Clone

CAT#: MC220204

Man1b1 (untagged) - Mouse mannosidase, alpha, class 1B, member 1 (Man1b1), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Man1b1
• Synonyms: E430019H13Rik; ERMan1; Gm108; MANA-ER
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC220204 representing NM_001029983
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTACCCACCGCCGGCGCCGCCGCCGGCGCCTCACCGGGACTTCATCTCGGTGACGCTGAGCCTGGGCG
  AGAGCTACGACAACAGCAAGAGCCGGCGGCGGCGCTCATGCTGGAGGAAATGGAAGCAGCTTTCGAGGCT
  ACAGCGGAATGTGATTCTCTTCGTCTTGGGCTTTCTGATTCTCTGTGGATTCCTGTACTCTCTTCATACG
  GCTGACCAGTGGAAAGCTCTGAGTGGCAGACCTGCAGAAGTAGAGAAGATGAAGCAAGAAGTCCTGCCTG
  TCTTACCAGCGCCTCAGAAGGAAAGTGCTGAACAAGAAGGGTTTGCAGACATACTCTCTCAGAAGCGGCA
  AAGGCATTTTCGGCGGGGCCCTCCCCATCTGCAGATTAGACCCCCCAACACAGTCTCCAAGGATGGGATG
  CAGGATGATGCTAAGGAGAGAGAAGCAGCCCTGGGGAAGGCTCAGCAGGAAGAAAACACACAGAGAACCG
  TCATCAGCTGGCGGGGAGCCGTGATTGAGCCTGAACAGGCCACTGAACTTCCTTACAAAAGAGCTGAAGC
  CTCCATCAAACCTCTGGTTTTGGCATCCAAGATATGGAAAGAGCCAGCCCCCCCGAATGAGCGCCAGAAG
  GGAGTGATTGAGGCCTTTCTCCATGCTTGGAAGGGTTACCAAAAGTTTGCTTGGGGCCATGATGAACTAA
  AACCTGTGTCCAAAACCTTCAGTGAGTGGTTTGGCCTGGGCCTCACCCTGATCGATGCCTTGGATACCAT
  GTGGATTTTGGGTCTGAAGCAAGAATTTAAGCAAGCCAGGAAATGGGTATCAGAGAACTTAGACTTTCAG
  AAGAATGTTGATGTCAACCTGTTTGAGAGCACCATTCGCATCTTAGGGGGCCTGCTGAGTACCTACCACC
  TGTCGGGGGACAGCCTCTTCCTGACCAAAGCTGAAGATTTTGGAAAACGGCTGATGCCTGCCTTCACTAC
  GCCCTCCAAGATCCCATACTCTGATGTGAACATTGGCACTGGATTTGCCCACTCACCCCAGTGGACTTCA
  GACAGCACGGTGGCAGAAGTGACGAGCATTCAGTTGGAGTTCCGGGAGCTCTCTCGTCTTACTGGAATAA
  AGAAATTCCAGGAGGCCGTGGAGGAAGTGACAAAGCACATCCACTCCCTGTCTGGGAAGAAAGACGGGCT
  GGTGCCTATGTTCATCAACACGAACAGCGGCCTCTTCACCCACCCAGGAGTGTTCACCTTGGGGGCCAGG
  GCTGACAGCTACTACGAATACCTGCTGAAACAGTGGATCCAGGGTGGCAAGAAGGAGACACAGTTGCTGG
  AAGACTATGTTAAAGCCATTGAGGGGATAAAAGCCCATCTGCTCCGGCAGTCTCAGCCCAGAAAGCTCAC
  CTTCGTGGGAGAACTTGCCCACGGCCGCTTCAGTGCCAAGATGGACCACTTAGTGTGCTTCCTGCCGGGG
  ACGCTGGCTTTGGGTGTCCACCATGGCCTGCCAGCTGACCACATGGATCTAGCTCGGGCCCTCATGGAGA
  CCTGCTACCAGATGAACCAACAGATGGAGACAGGTCTGAGCCCTGAGATTGCACATTTCAACATGTACCC
  TCGGGCAGATCATAAAGATGTGGAGGTCAAGCCAGCTGACAGGCACAACCTGCTGCGGCCAGAGACGGTG
  GAAAGCCTATTCTATCTGTACCGTGTCACAAGGGACCGCAAGTACCAGGACTGGGGCTGGGAAATCCTGC
  AGAGCTTCAATAAGTACACACGGGTCCCCTCAGGTGGCTATTCTTCTATCAACAACGTCCAGAATTCCCA
  CAAGCCTGAGCCCAGGGACAAGATGGAGAGCTTCTTTGTGGGGGAAACCCTGAAGTACTTGTACCTGCTC
  TTTTCTGATGACCTGGAGCTGCTCAGTCTGGACAGCTGTGTGTTCAACACAGAAGCCCATCCCCTCCCCA
  TCTGGGCTCCTGCGTAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001029983
• Insert Size: 1977 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001029983.2, NP_001025154.1
• RefSeq Size: 3748 bp
• RefSeq ORF: 1977 bp
• Locus ID: 227619
• UniProt ID: A2AJ15
• Cytogenetics: 2 A3
• Gene Summary: Involved in glycoprotein quality control targeting of misfolded glycoproteins for degradation. It primarily trims a single alpha-1,2-linked mannose residue from Man(9)GlcNAc(2) to produce Man(8)GlcNAc(2), but at high enzyme concentrations, as found in the ER quality control compartment (ERQC), it further trims the carbohydrates to Man(5-6)GlcNAc(2) (By similarity).[UniProtKB/Swiss-Prot Function]