D1Pas1 (NM_033077) Mouse Untagged Clone

CAT#: MC220194

D1Pas1 (untagged) - Mouse DNA segment, Chr 1, Pasteur Institute 1 (D1Pas1), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: D1Pas1
• Synonyms: Pl10
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC220194 representing NM_033077
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGTCATGTGGCAGAGGAAGATGAGCTCGGGCTGGACCAGCAGTTGGCTGGCCTAGACCTGACGTCTC
  GGGACAGCCAGAGCGGGGGCAGCACAGCCAGCAAAGGGCGCTACATCCCCCCTCACCTGCGGAACCGGGA
  GGCCGCGAAGGCGTTCTACGACAAAGACGGCTCACGGTGGAGTAAAGACAAGGATGCCTACAGCAGCTTC
  GGGTCCCGCAGCGACACGAGAGCCAAGTCCAGTTTCTTCAGTGACCGCGGCGGAAGCGGCTCCAGGGGAA
  GGTTTGACGAGCGCGGGCGGAGTGACTACGAGAGTGTTGGCAGCCGGGGAGGTAGAAGTGGCTTTGGCAA
  ATTTGAGCGGGGCGGGAACAGTCGCTGGTGTGACAAAGCAGACGAGGATGACTGGTCGAAACCGCTCCCA
  CCAAGCGAACGCTTGGAACAGGAACTCTTTTCCGGAGGCAACACTGGGATTAACTTTGAGAAATATGATG
  ACATCCCGGTTGAGGCGACAGGGAATAACTGCCCTCCACATATTGAAAGTTTCAGCGATGTCGAGATGGG
  AGAGATTATTATGGGAAACATCGAGCTCACTCGTTACACACGCCCAACTCCTGTGCAAAAGCATGCTATT
  CCTATTATCAAGGAGAAAAGAGACTTGATGGCTTGTGCCCAAACGGGGTCTGGAAAAACAGCAGCTTTTC
  TTCTACCTATCTTGAGTCAGATCTATACGGATGGTCCAGGTGAGGCTTTGAGGGCCATGAAGGAGAATGG
  AAAGTATGGGCGTCGCAAACAGTATCCAATCTCCTTGGTCTTGGCTCCGACCAGAGAATTGGCCGTGCAG
  ATCTATGAGGAGGCCAGGAAATTCTCATACCGCTCTCGAGTTCGTCCTTGTGTGGTTTATGGTGGAGCTG
  ATATTGGGCAGCAGATTCGAGACTTGGAACGTGGCTGTCACTTGTTAGTAGCTACTCCAGGACGGCTAGT
  AGATATGATGGAGAGAGGAAAGATTGGACTGGACTTCTGCAAATACTTGGTGTTAGATGAAGCAGATCGA
  ATGTTGGACATGGGCTTTGAGCCTCAGATACGAAGAATAGTTGAGCAAGACACCATGCCTCCAAAAGGTG
  TCCGCCACACTATGATGTTTAGTGCTACCTTTCCTAAGGAAATACAGATGCTGGCTCGGGATTTCTTGGA
  TGAATATATCTTTCTGGCCGTAGGAAGAGTTGGGTCTACTTCTGAGAACATCACACAGAAAGTCGTGTGG
  GTGGAAGAAGCAGACAAGCGGTCATTTCTGCTTGACCTCCTGAATGCAACGGGCAAGGATTCCCTGATAT
  TGGTGTTTGTGGAAACCAAAAAGGGTGCAGATTCTCTGGAGGACTTCTTATACCACGAAGGATATGCTTG
  TACCAGTATCCATGGAGACCGATCTCAGCGAGATAGGGAAGAGGCCCTTCACCAATTCCGATCAGGAAAA
  AGCCCAATTCTCGTGGCTACAGCAGTAGCTGCAAGAGGACTGGATATTTCCAATGTGAAACATGTCATCA
  ATTTCGACTTGCCTAGTGACATCGAAGAATATGTGCATCGCATTGGCCGTACAGGTCGTGTTGGAAACCT
  TGGCCTTGCCACCTCATTCTTCAACGAGAGGAACATAAATATTACCAAAGATTTATTGGATCTTCTTGTT
  GAAGCAAAACAAGAAGTGCCATCTTGGCTAGAAAACATGGCTTTTGAACACCATTACAAGGGTGGCAGTC
  GTGGACGGTCCAAGAGCAGATTTAGTGGAGGATTTGGTGCTAGAGACTATCGACAAAGTAGCGGTGCCAG
  CAGTTCCAGCTTCAGCAGTGGCCGTGCTAGCAACAGTCGCAGCGGTGGAGGCAGCCATGGCAGCAGCAGA
  GGGTTTGGTGGAGGTAGCTATGGAGGCTTTTACAACAGTGATGGATATGGAGGAAATTATAGCTCCCAGG
  GGGTTGACTGGTGGGGTAACTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_033077
• Insert Size: 1983 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_033077.3, NP_149068.1
• RefSeq Size: 3212 bp
• RefSeq ORF: 1983 bp
• Locus ID: 110957
• UniProt ID: P16381
• Cytogenetics: 1 90.09 cM
• Gene Summary: Putative ATP-dependent RNA helicase. Possible role in a key step of the spermatogenic process.[UniProtKB/Swiss-Prot Function]