Ano10 (NM_133979) Mouse Untagged Clone

CAT#: MC220190

Ano10 (untagged) - Mouse anoctamin 10 (Ano10), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Ano10
• Synonyms: AI604832; Tmem16k
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC220190 representing NM_133979
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGAGTGACTTTATCAACGCTGGATACTTGTGAGAGCTCCTTCACACCTCTAGTGGTCATAGAACTCG
  CCCAGGATGTCAAAGATGAAACCAAAGAATGGCTCAAAAACAGAATCATTGCCAAGAAGAAAGATGGAGG
  TGCCCAGCTATTGTTTAGACCACTGCTAAATAAGTATGAGAAAGAAACCCTAGAAAACCAGAATTTATAC
  CTTGTTGGTGCCTCCAATGTCCGACTGTTACTGGGGGCAGAAGCAGTCGGTCTGGTGAAGGAATGTACGG
  ACGCTGCCATGCGGGCCTTCACCTATGGAACACGCCACAACTTTAAAGGTTTCCATGATAACAATAATGA
  CTTCCTCACCATGGCTGAGTGTCAGTTCATTATCAAACACGAACTTGAGAATCTTAGAGCTAGAGACGAG
  AAGATGATCCCTGGCTACCCACAGGCCAAACTGTACCCTGGGAAGTCGCTGATGAGGAGACTGCTCACGT
  CTGGCATCGTGACCCAAGTGTTCCCGCTGCATGACACTGAAGCCCTGAAGAAGCTGGAGGACACGTGGTA
  CACTCGGTTTGCTCTCAAGTACCAGCCCATAGACAGCATTCGTAGCTACTTTGGGGAGACAATTGCTCTG
  TACTTCGGGTTCCTGGAGTACTTCACCTTTGCCTTAATCCCCATGGCCATCATTGGACTGCCCTACTACC
  TGTTTGTGTGGGAAGACTACGACAAGTATGTCATCTTTGCCTCGTTCAACCTCATATGGTCCACAGTGAT
  CCTGGAGGTGTGGAAGCGTGGCTGTGCCAACATGACCTATCGCTGGGGGACTCTGGTCATGAAGAGGCAG
  TTTGAGGAGCCCCGGCCAGGGTTTCATGGAGTCCTTGGCATCAATTCAGTCACGGGCCGGGAGGAGCCCC
  TCTACTCGAGCTACAAGAGACAACTGCGCATCTACCTAGTCTCTCTGCCATTTGTGTGCCTCTGCCTTTA
  TTTCTCCCTTTATGTCATGATGATCTACTTTGACATGGAGGACTGGGCCTTGAGTCTCCACGAGGACAGT
  GGGTCTGAGTGGACCAGCCTTCTGCTGTATGTACCCAGCATTGTTTATGCCGTTGTGATTGAGATCATGA
  ACCGCCTCTATCGATATGCTGCCGAGTTCCTAACATCTTGGGAGAATCACAGATTGGAATCTGCCTACCA
  GAATCATCTCGTTCTGAAAGTGTTGGTGTTCAACTTCCTGAATTGCTTCGCCTCACTCTTCTACATTGCC
  TTTGTCCTGAAGGACATGAAGCTTTTGCGCCAGAGCTTGGCCACACTCCTGATCACCTCCCAGATTCTGA
  ACCAAGTCGTAGAATCTCTTCTTCCTTACTGGCTGCAGCGGAAGTACTGTGCGAGGGTAAAGCGGAAGGT
  TCAGGCATTAAAGTCTGAGGTCGACACAACCTTGTATGAGCAAGTCCTCCTGGAGAAGGAAATGGGAACG
  TACCTGGGAACCTTTGATGATTACCTGGAGTTGTTCCTGCAGTTTGGCTATGTGAGCCTTTTCTCTTGTG
  TTTACCCGCTAGCAGCTGCCTTTGCTGTGTTAAATAACTTCACTGAAGTCAACTCAGATGCCTTGAAAAT
  GTGCAGGGTCTTCAAACGGCCATTTGCAGAACCTTCGGCCAGTATTGGCGTATGGCAGTTGGCTTTTGAA
  ACGATGAGCGTTATATCCGTGGTCACTAACTGTGCTCTGATTGGAATGTCACCACAAGTGAATGCAGTCT
  TTCCAGAATCCAAAACAGACCTTGTTCTGATTGTGGTGGCCGTAGAGCATGCACTCTTGGCTCTGAAGTT
  CATACTCGCATTTGCCATCCCTGATAAACCACGGCACATCCAACAGAAACTGGCCAGATTGGAATTCGAA
  TCTTTGGAGGCACTCAAGCAACAGCAAATGAAGCTGGTGGCAGAAAACCTGAAGGAGGAGTACCAGGAAG
  ATGGGAAGGAAGCTACCTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_133979
• Insert Size: 1980 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_133979.3, NP_598740.1
• RefSeq Size: 2653 bp
• RefSeq ORF: 1980 bp
• Locus ID: 102566
• UniProt ID: Q8BH79
• Cytogenetics: 9 F4
• Gene Summary: This gene encodes a member of the anoctamin family, which in mammals is comprised of 10 members. Anoctamin proteins are proposed to have eight transmembrane domains with both termini facing the cytoplasm and a C-terminal domain of unknown function. While some members have been characterized as calcium-activated chloride channels, this protein is reported to inhibit anion conductance. Alternative splicing results in multiple transcript variants that encode different protein isoforms. [provided by RefSeq, Dec 2012]
  Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.