Sass6 (NM_028349) Mouse Untagged Clone

CAT#: MC220186

Sass6 (untagged) - Mouse spindle assembly 6 homolog (C. elegans) (Sass6), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Sass6
• Synonyms: 2810453L12Rik
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC220186 representing NM_028349
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGTCAAGTTCTGTTCCAGCAGCTTGTTCCCTTGCTAGTGAAATGCAAAGACTGCGAGGAGAGGAGAG
  GGAGCGTTAGAGTGAGCATTGAGCTGCAGTCCCTTTCGAATCCAGTACACAGGAAGGATTTAGTCATCCG
  TCTGACTGATGATACAGATCCGTTTTTTCTGTATAACCTTGTTATATCTGAGGAAGATTTTCAAAGTTTA
  AAATTGCAGCAAGGTCTCCTGGTGGACTTCTTAGCTTTCCCACAGAAGTTTATAGACCTCCTTCAGCAGT
  GTATGCAAGAACACGCGAAAGAGACTCCAAGGTTCCTGCTGCAGCTTCTTTCTTCAGCCACTCTTTTGGA
  GAACTCACCGGTCCTTTTAAACGTAGTGGAGACAAATCCTTTTAAGCATCTTATTCACCTGTCACTAAAG
  CTTTTACCTGGAAATGATGTAGAAATAAAGAAATTTCTAGCAGGATGTTTGAAATGTAGCAAGGAAGAAA
  AATTATCACTGACTAGATCACTAGATGATGTTACCAGGCAACTGCACATTACACAAGAGACGTTGTCGGA
  AAAAATGCAAGAATTAGATAAGCTGCGGAGTGAATGGGCCTCGCACACAGCATCGCTGACGAATAAGCAC
  TCTCAGGAGTTAACAGCTGAGAAGGAGAAGGCCTTGCAGACCCAAGTTCAGTGCCAACAGCAGCACGAGC
  AACAGAAAAAGGAACTGGAAACCCTCCATCAACGGAATATCCACCAGCTACAAAGCAGATTGTCCGAGTT
  AGAGGCAGCTAATAAAGAGCTCACCGAGAGGAAGTATAAAGGAGACTCGACTGTCCGAGAGCTAAAGGCG
  AAGCTGGCTGGCGTGGAAGAGGAGCTGCAGCGGGCCAAGCAAGAGGTCCTTTCTCTGAGAAGAGAGAATT
  GTACTCTGGATACTGAATGCCACGAGAAAGAAAAGCACATCAACCAGCTACAAACAAAAGTGGCCGTTTT
  AGAACAGGAGATCAAAGATAAAGACCAGCTTGTTCTGAGAACAAAAGAAGCATTCGATACAATCCAAGAG
  CAAAAGGTGGCTTTAGAAGAAAATGGTGAGAAAAATCAGATACAACTGGGAAAACTTGAAGCTACAATAA
  AATCATTATCAGCAGAACTTCTAAAGGCAAATGAGATCATCAAGAAGTTACAAGGAGATCTTAAAACTCT
  GATGGGTAAATTGAAACTGAAGAATACAGTTACTATTCAGCAAGAAAAACTATTGGCTGAGAAAGAAGAA
  ATGCTACAAAAGGAGCGAAAGGAATCACAGGACGCTGGGCAGTTTCTTCGTGCCAAAGAGCAAGAGGTAT
  GCAGATTACAGGAACAATTAGAAACTACAGTTCAGAAACTCGAAGAAAGTAAACAACTTTTGAAAAATAA
  TGAAAAATTAATCACGTGGCTAAACAAAGAGCTAAATGAAAATCAGCTGGTAAGAAAACAGGACACATTG
  GGAACCTCTGCCACCCCACATTCTACTAGCAACAGCACCATCAGAAGTGGGCTCTCTCCCAATCTGAATG
  TGGTTGATAGACTAAATTACCCAAGCTGTGGAATTGGCTATCCTGTCTCCTCTGCATTGACATTCCAGAA
  TGCTTTTCCTCATGTAGTAGCTGCCAAAAACACCAGCCACCCTATCTCTGGACCAAAGGTTCACTTTAAC
  CTGCAGCTTACAAAACCAAGCGCTTCGATAGATGGGCAGCCGGGAGCCGCTGTTAACAGGCCTTGTTCAA
  ATGATAAGGAAAATGGTGAAACTTTAGGATTGGAATCCAAATACTTGAAGAGAAGAGAAGCTAGCATTCC
  TTTACGCGGACTTAGCCAGAATCTGTTGAGTGACTCAGACCATCAGAAAGACGGCATGCTGGGAGCGTTC
  CAGCTATCTTCCAAACCCACCGTTCTCCCCTCCTCGTCTTCAGCCTACTTCCCTGGGCAGTTACCAAGTA
  GTTAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_028349
• Insert Size: 1965 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_028349.3, NP_082625.2
• RefSeq Size: 4309 bp
• RefSeq ORF: 1965 bp
• Locus ID: 72776
• UniProt ID: Q80UK7
• Cytogenetics: 3 G1
• Gene Summary: Central scaffolding component of the centrioles ensuring their 9-fold symmetry. Required for centrosome biogenesis and duplication: required both for mother-centriole-dependent centriole duplication and deuterosome-dependent centriole amplification in multiciliated cells (PubMed:24240477). Required for the recruitment of STIL to the procentriole and for STIL-mediated centriole amplification (By similarity).[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (1) encodes the longest isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.