Cacnb2 (NM_023116) Mouse Untagged Clone

CAT#: MC220150

Cacnb2 (untagged) - Mouse calcium channel, voltage-dependent, beta 2 subunit (Cacnb2), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Cacnb2
• Synonyms: AW060387; CAB2; Cavbeta2; Cchb2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC220150 representing NM_023116
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGTCCAAAGCGACACGTCCAAGTCGCCTCCCGTAGCGGCGGTGGCGCAGGAGAGCCAGATGGAGCTGC
  TGGAGAGCGCGGCTCCCGCGGGGGCGCTCGGAGCACAGTCCTATGGAAAAGGAGCCAGGAGGAAAAATAG
  ATTTAAAGGCTCGGATGGAAGCACATCGTCAGACACTACCTCAAACAGTTTTGTCCGCCAGGGTTCGGCA
  GACTCCTACACCAGCCGCCCATCCGATTCAGATGTGTCTTTGGAAGAGGACCGGGAGGCAGTACGTAGAG
  AAGCTGAGCGGCAGGCACAGGCACAGTTGGAAAAAGCAAAGACAAAACCTGTTGCATTTGCAGTTCGGAC
  CAATGTCAGATACAGCGCAGCCCAGGAGGATGACGTTCCGGTGCCTGGCATGGCCATCTCCTTCGAGGCC
  AAAGATTTTCTGCATGTTAAAGAAAAATTTAATAATGACTGGTGGATAGGACGGCTGGTTAAAGAAGGCT
  GTGAAATCGGATTTATTCCAAGCCCAGTCAAACTAGAGAACATGAGGCTACAGCACGAACAGAGAGCCAA
  GCAAGGGAAATTCTATTCCAGTAAATCAGGAGGGAATTCATCATCCAGTTTGGGTGACATAGTGCCCAGT
  TCCAGAAAATCGACACCGCCGTCATCTGCTATAGACATAGATGCTACTGGCTTAGATGCAGAAGAGAATG
  ATATTCCAGCAAACCACCGCTCCCCTAAGCCCAGTGCAAACAGTGTAACATCACCCCACTCCAAAGAGAA
  AAGAATGCCCTTCTTTAAGAAGACAGAGCACACTCCTCCTTATGATGTGGTACCTTCCATGCGACCAGTG
  GTGCTGGTGGGCCCGTCTCTGAAGGGGTATGAGGTCACAGATATGATGCAAAAAGCACTGTTTGATTTTC
  TAAAACACAGATTTGAAGGGCGGATATCCATCACAAGAGTCACTGCTGACATCTCCCTTGCCAAACGCTC
  AGTATTAAACAATCCCAGTAAGCATGCAATCATAGAAAGATCCAACACAAGGTCCAGCTTAGCGGAAGTT
  CAGAGTGAAATTGAAAGGATTTTTGAACTTGCAAGAACACTGCAATTGGTAGTCCTTGACGCGGATACAA
  TTAATCACCCAGCTCAACTCAGTAAGACCTCTTTGGCCCCTATCATAGTGTACGTAAAGATTTCTTCTCC
  CAAGGTTTTACAAAGGTTGATAAAATCTCGAGGGAAATCTCAAGCAAAACACCTCAATGTCCAGATGGTA
  GCAGCTGATAAACTGGCCCAATGTCCTCCGCAGGAATCATTTGATGTGATCTTGGATGAGAACCAGCTGG
  AGGACGCTTGTGAGCATCTTGCCGACTATCTGGAGGCATACTGGAAGGCCACCCACCCTCCCAGCGGTAA
  CCTCCCCAACCCTCTGCTTAGCCGGACTTTAGCCTCCTCAACTTTACCTCTGAGCCCCACCCTCGCCTCT
  AATTCACAGGGTTCTCAAGGTGATCAAAGGCCTGATCGCTCTGCTCCCCGGTCTGCTTCCCAAGCTGAAG
  AAGAACCTTGCCTAGAACCCGTCAAAAAATCCCAACACCGTTCCTCCTCAGCCACACACCAAAACCACCG
  CAGCGGGACAGGTCGAGGCCTCTCTAGGCAAGAGACGTTTGACTCTGAAACCCAAGAGAGCCGAGACTCT
  GCCTACGTGGAGCCAAAGGAAGATTATTCACATGAACATGTGGACCGGTATGTCCCACACCGCGAGCATA
  ACCACAGAGAGGAGACCCACAGCAGCAATGGCCACAGACACAGGGAGTCTCGCCACCGCTCTAGGGACAT
  GGGTCGAGACCAGGACCACAATGAGTGCATCAAACAACGAAGCCGGCATAAATCTAAGGATCGCTACTGT
  GACAAGGAGGGGGAAGTAATATCCAAGAGAAGGAATGAGGCTGGCGAGTGGAACAGGGATGTATACATCC
  GCCAATGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_023116
• Insert Size: 1968 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_023116.4, NP_075605.1
• RefSeq Size: 3945 bp
• RefSeq ORF: 1968 bp
• Locus ID: 12296
• UniProt ID: Q8CC27
• Cytogenetics: 2 10.7 cM
• Gene Summary: The beta subunit of voltage-dependent calcium channels contributes to the function of the calcium channel by increasing peak calcium current, shifting the voltage dependencies of activation and inactivation, modulating G protein inhibition and controlling the alpha-1 subunit membrane targeting.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1).