Nsd2 (NM_001177884) Mouse Untagged Clone

CAT#: MC220109

Whsc1 (untagged) - Mouse Wolf-Hirschhorn syndrome candidate 1 (human) (Whsc1), transcript variant 3, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Nsd2
• Synonyms: 5830445G22Rik; 9430010A17Rik; AW555663; C130020C13Rik; D030027O06Rik; D930023B08Rik; mKIAA1090
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC220109 representing NM_001177884
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAATTTAGCATCAGAAAAAGTCCTCTTTCTGTTCAGAAAGTTGTAAAGTGCATGAAGATGAAGCAGA
  CACCAGAAATCCTTGGCAGTGCAAATGGAAAGACTCAGAACTGTGAAGTGAATCATGAATGTTCTGTATT
  CCTCAGCAAAGCTCAACTTTCTAACAGCCTACAGGAGGGGGTCATGCAGAAATTTAATGGCCATGATGCG
  CTCCCCTTTCTTCCAGCAGAGAAGTTGAAAGATCTTACTTCTTGTGTTTTTAATGGAGAACCTGGTGCTC
  ATGATACTAAATTGTGTTTTGAGGCCCAGGAAGTAAAAGGAATTGGGACACCACCCAATACTACACCTAT
  CAAAAATGGCTCTCCAGAAATTAAACTGAAAATCACCAAAACATACATGAATGGGAAACCTCTCTTTGAA
  TCTTCAATTTGTGGTGACGGTGCTGCTGATGTGTCTCAGTCAGAAGAAAACGAACAAAAATCTGATAATA
  AGACTAGGAGGAACAGGAAGAGGAGCATAAAATATGACTCTTTACTGGAGCAGGGCCTTGTGGAAGCAGC
  TTTAGTGTCCAAGATCTCAAGTCCTGCAGATAAAAAGATTCCAGTTAAGAAGGAGTCCTGTCCAAATACT
  GGCAGAGACAGAGACCTCTTGTTAAAATACAACGTTGGTGATTTGGTGTGGTCCAAAGTGTCAGGTTACC
  CTTGGTGGCCTTGCATGGTTTCTGCTGACCCACTCCTTCACAATCATACCAAACTTAAAGGTCAGAAAAA
  AAGTGCACGCCAGTATCATGTACAGTTCTTTGGTGATGCCCCAGAAAGAGCTTGGATATTTGAGAAGAGC
  CTTGTTGCTTTTGAAGGAGAAGAACAATTTGAAAAATTATGCCAAGAAAGTGCCAAGCAGGCACCCACGA
  AAGCTGAGAAAATCAAGTTGTTGAAACCTATTTCGGGGAGATTGAGAGCCCAGTGGGAAATGGGCATTGT
  CCAAGCTGAGGAAGCTGCCAGCATGTCAATAGAGGAGCGGAAAGCCAAATTTACCTTCCTTTATGTGGGG
  GACCAATTGCACCTCAACCCTCAAGTAGCTAAGGAGGCTGGCATTGTTACAGAACCTTTGGGAGAAATGG
  TGGACTCTTCAGGGGCCAGTGAAGAGGCTGCTGTAGACCCTGGGTCTGTGAGAGAAGAGGATATTCCCAC
  GAAGAGAAGGCGAAGAACCAAAAGGTCTAGTTCTGCTGAGAACCAAGAAGGTGATCCTGGCACAGACAAG
  AGTACACCTCCAAAGATGGCAGAGGCTGAACCTAAGCGAGGAGTAGGCTCTCCTGCTGGGAGGAAAAAGT
  CCACAGGCTCTGCTCCTCGGAGCAGGAAGGGAGACTCAGCAGCCCAGTTTTTAGTCTTTTGTCAAAAACA
  CAGAGATGAGGTTGTAGCTGAACATCCAGATGCCTCAGGGGAAGAGATTGAAGAATTGCTTGGGTCCCAG
  TGGAGCATGCTCAATGAAAAGCAGAAAGCACGATATAATACAAAGTTTTCCCTAATGATCTCTGCCCAGT
  CTGAAGAAGACTCTGGAAATGGGAATGGGAAAAAAAGAAGCCACACAAAGAGAGCAGATGACCCTGCAGA
  GGATGTGGATGTTGAAGACGCGCCCAGGAAAAGACTTAGAGCAGATAAGCACAGTCTTCGGAAGCAGAGA
  GAGACAATCACCGACAAAACGGCCAGAACAAGCTCTTACAAGGCTATAGAGGCAGCCTCCTCACTCAAGA
  GCCAGGCAGCAACGAAAAATTTGTCTGATGCTTGCAAACCACTGAAGAAGCGAAATCGGGCTTCTGCAAC
  AGCGTCTTCAGCACTTGGGTTTAACAAAAGTTCATCTCCTTCTGCATCCTTAACTGAGCATGAGCTTTTA
  TGGGAGCCCACATCAGTCAAGTTGGACTTGAACTCAGCCGCCCTGTACTGCACTTAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001177884
• Insert Size: 1947 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001177884.1, NP_001171355.1
• RefSeq Size: 7806 bp
• RefSeq ORF: 1947 bp
• Locus ID: 107823
• Cytogenetics: 5 B2
• Gene Summary: Histone methyltransferase with histone H3 'Lys-27' (H3K27me) methyltransferase activity.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (3) differs in the 3' coding region and 3' UTR, compared to variant 1, resulting in an isoform (3) with a distinct and significantly shorter C-terminus, compared to isoform 1. The 5' UTR of this variant may be incomplete due to the lack of 5'-complete transcripts supporting it, and the presence of alternative splicing choices further upstream. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.