Pik3c2g (NM_011084) Mouse Untagged Clone

CAT#: MC220068

Pik3c2g (untagged) - Mouse phosphatidylinositol 3-kinase, C2 domain containing, gamma polypeptide (Pik3c2g), transcript variant 2, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Pik3c2g
• Synonyms: C80387; PI3K-C2-gamma
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC220068 representing NM_011084
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGGCTGCTACGGGATGCCCAAGGTGAAGCCTACTTTAAAAGCTGGTATCAGGAGCTTTTGGCCGCTC
  TCCAGTTCTGTGCAGGAGAAGCCCTGAATGAAGAGCTTTCCAAGGAGCAGAAACTTGTCAAACTTCTGGG
  TGATATTGGAGAAAAAGTGAAGTCAGCCAGTGACCCTCAGAGAAAGGATGTGCTAAAGAAAGAGATTGGC
  AGTCTAGAAGAATTCTTTAAAGATATAAAGACTTGCCATCTTCCTCTGAACCCGGCACTGTGCATAAAAG
  GAATTGATCGGGATGCTTGCTCGTATTTCACATCCAACGCTTCGCCATTGAAGATTACTTTCATCAATGC
  TAATCCAATGGGCAAAAATATCAGTGTTATTTTTAAGGCCGGAGATGATCTTCGGCAGGATATGCTTGCT
  CTGCAGATTATTCAAGTGATGGACAACGCTTGGCTGCAGGAGGGCCTCGATATGCAAATGATCACTTATG
  GATGTCTATCCACAGGAAGAGCCCAAGGATTCATAGAGATGGTGCCTGATGCTGTAACCCTGGCCAAGAT
  CCATCTGCACTCTGGGCTGATCGGACCCCTGAAAGAAAACACCATCAAGAAGTGGTTCAGTCAGCACAAC
  CACTTAAAGGAAGATTATGAGAAGGCCTTGAGGAACTTCTTTTACTCTTGTGCTGGCTGGTGTGTGGTGA
  CATTCATCTTGGGAGTCTGTGACCGACATAATGACAATATCATGCTGACAAAGTCAGGTCACATGTTTCA
  TATTGACTTTGGAAAATTCTTGGGTCATGCGCAAACATTTGGCGGTATCAAAAGGGACAGAGCCCCTTTC
  ATTTTTACTTCAGAGATGGAGTACTTTATTACTGAGGGTGGAAAAAACACACAGCATTTTCAAGACTTCG
  TGGAGCTTTGCTGCAGAGCTTACAACATCGTCAGGAAACACAGCCAGTTGATCCTGAGCCTTCTAGAGAT
  GATGCTGCATGCAGGACTGCCTGAGCTAAGGGGAATCGAAGACCTGAAGTATGTACATAACAATCTTCGC
  CCACAAGACACAGACCTGGAAGCCACAAGTCATTTCACCAAGAAGATAAAGGAAAGTCTGGAGTGTTTCC
  CAGTGAAATTGAATAACCTGATCCACACACTTGCACAGATGCCAGCCTTAAGCCTTGCCAAACCTGCCCC
  TCAGACTCTTCTCCAGGAATCCTGCATCCTGAATAAGACCAGGACAATTCAGAGAGTCACAATTTTAGGG
  TTCAGCAAGACACACAGCAACCTGTACCTGATGGAGGTGACATGCAGTGACAATAGGAGAAGCCTGACCA
  AGAAGTCCTTCGAGCAGTTTTACAGACTTCACAGCCAAATGCAGAAGCAGTTCTCCTCCTTGGCTCTCCC
  AGAATTTCCTCACTGGTGGCATCTACCTTTCACAGACTCAGATCACAAGAGAATCAGAGATCTGAGTCAC
  TACGTGGAACAGGTGCTACGCGGATCTTATGAAGTTGCAAACAGTGATTGTGTACTTAGCTTTTTTCTCT
  CTGAACATATTCAACCAACACTTGAAGACTCTCCATTTGTGGACCCAGGTGAGAACTCTCTAGACAAGAG
  CCCTAAGGTGCAGTTACTGATGACGTATGAGGACTCGAGGCTCACCATCCTAGTGAAACACTTGAAAAAC
  ATCCATCTCCCAGATGGCTCTGTGCCCAGTGCACATGTTGAAATTTATCTTCTACCACATCCCAGTGAAG
  TTCGGAGAAAGAAAACAAAGTGTGTTCCAAAATGCACTGACCCAACTTACAATGAAATTGTGGTATATGA
  TGAAGTCTTGGGGCTCCAGGGCCATGTTTTAATGCTTATCGTGAAGAGCAAAACTGTATTTGTGGGAGCA
  GTTAACATCCAGCTCTGCAGTGTACCCCTCAATGAAGAAAAGTGGTACCCACTAGGAAACAGTATCATCT
  GA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_011084
• Insert Size: 1962 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_011084.2, NP_035214.2
• RefSeq Size: 3549 bp
• RefSeq ORF: 1962 bp
• Locus ID: 18705
• Cytogenetics: 6 69.7 cM
• Gene Summary: Generates phosphatidylinositol 3-phosphate (PtdIns3P) and phosphatidylinositol 3,4-bisphosphate (PtdIns(3,4)P2) that act as second messengers. May play a role in SDF1A-stimulated chemotaxis.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (2) contains multiple differences, compared to variant 1, including the lack of multiple 5' coding exons. It initiates translation at an alternate start codon. The encoded isoform (2) is shorter and has a distinct N-terminus, compared to isoform 1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments.