Shc4 (NM_199022) Mouse Untagged Clone

CAT#: MC219880

Shc4 (untagged) - Mouse SHC (Src homology 2 domain containing) family, member 4 (Shc4), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Shc4
• Synonyms: 6230417E10Rik; 9930029B02Rik; Gm685; RaLP
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC219880 representing NM_199022
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCGAGAACGCAGCCAGGACAGCCAGGCAGGACTCACGCTGTATGTAGGACTCTTTGGGCACCTGGGGA
  TGCTTCACAGGACCAAGTACAGCCGCTTTCGGAACGAGTCCATCACGTCTTTGGACGAAGGGAGCCCCGG
  AGGCTCCGTTGGAAACAAGGGCTCGTCGCCGCCTCCCTACCCAGCCCTGGCCCCTCACCTGCCGACTGAG
  GATGCCACTGTGTCCAGCCAGGAGAGCCCCACGGCGCTGTGCACCTTGATTCCCCGTATGGCAAGCATGA
  AGCTGGCCAACCCCATCACTTTTCTGGGTCTGAAAACCTTCTGCTTGGGTACGAAACAGGTGAGCCGGTT
  GAAGCTCCAAGAGAACCAGGATCAGACTCCAAGCCGTCCAGCGTCCCCAGAATCCAATCTAAACAGGACT
  GGACCTGCACCTGCACCGGATCCGGACCAGGTGGGACGCAGGCCCACCTCTTTGCGTCCTGATACTTGCC
  CACTGCCTGGCCCTGGAGAACCAAGCCCCAGGAGCAAGCAGGACGGGCCCCCTCTGCAGCACCTGTTGGG
  GAACGGTCTGAACTACTGTGTGCGGTACATGGGCTGCATTGAAGTGCTGCAATCAATGAGGTCACTGGAT
  TTTGGAATGAGAACCCAAGTTACAAGGGAAGCAATAAGTCGCCTGTGTGAAGCTGTCCCCGGGGCCCATG
  GAGCCATTAAAAAACGAAAGGCTCCCGTTAAGTTCCTGACAACGGTTCTTGGCAAAAGCAATCTTCAGTT
  TTCAGGAATGAATATAAAACTGACTGTTTCAACAAGCAGCCTCACACTGATGAACCTTGACAACCAACAG
  ATTATTGCAAATCACCAAATGCAATCGATTTCATTTGCCTCTGGAGGGGACCCTGATACCACAGACTATG
  TTGCCTATGTAGCTAAAGATCCAGTCAACCAACGAGCGTGCCACATACTGGAGTGTCGCAGTGGAATGGC
  CCAAGACGTCATAAGCACCATAGGGCAGGCCTTTGAACTCCGGTTCAAACAGTACTTGAAAAATCCTTCC
  TTGAACACTTGGGAAAGGGAGGAGGTACTTGTTGATGGTGCCCCTGAAGACAGAGACCATGACTATTACA
  ACTCCATCCCGGGGAAGCAGCCACCTGAAGGCGGCATTTCAGATGTGCGCATCCAGGCTCAAGCTACAGA
  CCAAATGGCATACTGCCCTATCCGGTGTGAAAAGTTGTGCTATTTGCCTGGGAACTCCACGTGCAGCGGT
  GTGTATAAGAACTGTATGGGACGGAGCAGGCCAATAGGTATCCCGCACGAGAGAGCGGGGCAGGGTGACA
  CACCATCGCTGAGGCACTTCTGGAGAGTGGATCTCTTTGACGACCCCTGCTATGTCAATACACAGGCTCT
  TCAGAGTATGCACAGCTATGCTGGAAATCAAAGCTCAGCTCTGCCACAGGGGAGCCCCTGGCACCTCGGA
  AAGGCACCAGAAACTGTGCAGCCTGGAGCCACGGCCAAGCCTGGGAGCGCACTCGCTTTGCCACACATTA
  GGCAGCAGCTGTGGGACGAGGAGTGCTTCCATGGCAAGCTGAGCAGAGGGGCAGCGGAGAAACTTTTGGT
  GAAGGATGGGGACTTTTTGGTTCGAGAGAGTGTGACATCTCCCGGCCAGTTTGTACTGAGTGGACTGCAA
  GGTGGCCAGGCCAAGCATCTTCTCTTGGTGGATCCAGAAGGCAAGGTGAGGACCAAGGACCATGTGTTTG
  ATAATGTTGGCCACCTTATCAAATACCATATGGATAATAATTTGCCAATCATCTCTTCTGGAAGTGAAGT
  ACGCCTTAAACAACCAATAAGAAAATATGATAACACAGGACTTTTGCCCCCCAAGAAATGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_199022
• Insert Size: 1881 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_199022.2, NP_950187.1
• RefSeq Size: 4820 bp
• RefSeq ORF: 1881 bp
• Locus ID: 271849
• UniProt ID: Q6S5L9
• Cytogenetics: 2 F1
• Gene Summary: Activates both Ras-dependent and Ras-independent migratory pathways in melanomas. Contributes to the early phases of agrin-induced tyrosine phosphorylation of CHRNB1.[UniProtKB/Swiss-Prot Function]