Mmp25 (NM_001033339) Mouse Untagged Clone

CAT#: MC219769

Mmp25 (untagged) - Mouse matrix metallopeptidase 25 (Mmp25), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Mmp25
• Synonyms: F730048C11Rik; Leuk; Leukolysin; MMP20; MT6-MM; MT6-MMP
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC219769 representing NM_001033339
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTGCTTCCCAGGATCCCAGATCTCTCCTGCCCGTCTCTACTACCTTGTGTCGGCTCCCTGGATCTGCA
  CCGGGTCCCTGACCTCCTCCAGACTTCCACGGCGGCGGGAGTCAGGGCCTCTTCGCGTGCCTCCGCGGTC
  CGTCCAAGCTGAACGCATCTTGAGGCTGCCGGCTTTCGGTCTCCCGTTGCTGGCGCTTCTGTTGGTTCCG
  CTGCTGCCGGTGCGCGCTCAGAATCCCGATGCGAAGGTCGTGAGCATGGGCGTGGAATGGCTGACTCGCT
  ATGGCTACCTGCCACCGGCGGATCCTGTCCACGCCCAGATGCAGAGTCTTGAGAAACTGCAGGATGCAAT
  CAAAGTCATGCAGAGATTTGCCGGACTACCTGAGACTGGACAAATGGACCCAATGACAATAAAGACCATG
  CGCAAGCCCCGGTGCTCCCTGCCTGATGTCCTGGGCGCAGCTGGGCTGGTCAGACGCCGACGTCGCTATT
  CTCTGAGTGGCAGTGTTTGGAAGAAACGCACCCTGACATGGAGTATCCGGTCCTTCTCTCAGAAATCCCA
  GTTGAGCCCGCAGATTGTTCGGACCCTCCTGAGCTATGCCCTGGCTGTCTGGGCTACTGAATCAGGCCTT
  ACATTCCAGGAGGTGAATTCTCAGTACCAGGAGCCTGACATCATTATCCACTTTGCTCGGGCCTACCACC
  AGGACAGTTACCCCTTTGACGGGTCTGGTGGCACGTTGGCTCACGCCTTCTTCCCTGGGGAGCACCCTAT
  CTCTGGAGACACGCACTTTGATGATGAAGAGACCTGGACTTTTGGGTCAACAGATGATAACGGAATTGAC
  CTATTTGCAGTAGCAGTTCATGAATTTGGCCATGCCCTAGGCCTTGGCCACTCTTCTGCACCCAACTCCA
  TTATGCGGCCCTTCTACCAGGGCCCGGTGGGTGACCCTGCCACATACCGTTTGCCCCAGGATGACCGTGA
  CGGACTGCAGCAGCTCTATGGGAGAGTGTCCCAAAATCCAAATGCCAGGCCCACAAGGAAACCCCTGGTT
  CCGCCTCCCCAGCCTCCAGCCATGCCCCCTGACAGCCCCGCCACACCTGTGCCTGACCGCTGTGAGGGCA
  ACTTTGATGCCGTTGCCAACATTCGAGGGGAAATTTTCCTCTTCAAAGGCCCATGGTTTTGGCGCCTCCA
  ACCCTCAGGACAGCTGGTATCACCCCGCCCTGCTGGGTTGCACCGCTTTTGGGAGGGGCTGCCAACCCAT
  GTGAAAGTCATCCAGGCTGCCTATGCCCGACCCCTAGATGGCCGAATCATCCTCTTCAGCGGCCCGCAGT
  TCTGGGTGTTCCAGGAACGACAGCTTGAGGGTGCAGCGCGGCCACTGGTAGAGTTTGGGCTGCCCCCAGG
  AGAAGACGTGGATGCTGTGTTCTCATGGCCTCACAATGGCAAGACATACCTGATCCGAGGTCAAAAGTAC
  TGGCGGTATGACGAGGTGGCCGCCCGCCCAGACCCTGGCTACCCACGTGCCTTGAGTCTCTGGGATGGGG
  CGCCCTTTGCCCCAGACGATGTCACCATCAGCAACACAGGTGACACTTACTTTTTCAAGGGCACCCATTT
  CTGGCGCTTTGCGGAGGGCAGTGTCAAAGCTGAGTCAGATTCCCCCCAGCCCATTGGGCCCAAGTGGCTG
  GACTGCCCTGCCCCCAACTCTGACCCTCGAGTCACCAGTCCCCCGAAAACGACATCCAAAACCCGAAGCT
  GTGATTGTCACTGCGAGCTCAATCAAGCCTCAGAGCAGCTGTCGCCCCTCCTGCTGCCTCTCCTACCCCT
  AGTGGCTGGAGAGGTCTTTTCTTACTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001033339
• Insert Size: 1848 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001033339.4, NP_001028511.1
• RefSeq Size: 3670 bp
• RefSeq ORF: 1848 bp
• Locus ID: 240047
• UniProt ID: Q3U435
• Cytogenetics: 17 A3.3
• Gene Summary: This gene encodes a member of the matrix metalloproteinase family of extracellular matrix-degrading enzymes that are involved in tissue remodeling, wound repair, progression of atherosclerosis and tumor invasion. The encoded precursor undergoes proteolytic processing to generate a mature, zinc-dependent endopeptidase enzyme. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Feb 2016]
  Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.