Msl1 (NM_028722) Mouse Untagged Clone

CAT#: MC219735

Msl1 (untagged) - Mouse male-specific lethal 1 homolog (Drosophila) (Msl1), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Msl1
• Synonyms: 2810017F12Rik; 4121402D02Rik; 4930463F05Rik; AA682082; Msl-1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC219735 representing NM_028722
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGACCATGAGATCCGCAGTGTTCAAGGCGGCCGCGGCCCCTGCCGGCGGCAACCCCGAGCAGCGACTGG
  ACTACGAGCGGGCTGCGGCGCTGGGCGGGCCCGAGGACGAGTCCGGGGCGGCCGAAGCCCATTTCCTCCC
  CCGGCATCGTAAGCTCAAGGAGCCGGGGCCCCCGCTGGCCTCTTCCCAGGGCGGGAGCCCCTCGCCCTCT
  CCAGCCGGCTGCGGCGGCGGCAAGGGCCGGGGCTTGTTACTCCCAGCCGGGGCGGCCCCCGGGCAGCAGG
  AAGAGAGCTGGGGCGGTTCGGTGCCCTTGCCCTGTCCGCCCCCAGCTACTAAACAAGCCGGCATCGGCGG
  GGAGCCAGTCGCAGCCGGCGCTGGCTGCAGCCCCCGGCCCAAGTATCAGGCGGTGCTGCCCATTCAGACG
  GGCTCTATCGTGGTGGCGGCGGCCAAAGAGCCTACGCCCTGGGCTGGGGACAAGGGTGGGGCGGCTCCCC
  CAGCTGCCACCGCCTCGGACCCGGCGGGACCCCCACCACTACCTCTGCCCGGGCCACCACCCCTCGCGCC
  CACCGCCACTGCTGGGACCCTGGCGGCCAGTGAGGGCAGATGGAAGAGTATAAGGAAGAGCCCTCTCGGG
  GGTGGCGGCGGCTCGGGAGCCTCCAGTCAGGCCGCCTGCCTCAAACAGATCCTTCTGCTGCAATTGGACC
  TCATCGAACAGCAGCAGCAGCAGTTGCAGGCCAAGGAGAAGGAGATAGAGGAGCTGAAGTCCGAGAGAGA
  TACGCTCCTTGCTCGGATTGAACGTATGGAAAGGCGGATGCAGCTGGTGAAGAGGGATAACGAGAAGGAA
  AGGCACAAGCTGCTCCAGGGCTATGAACCTGAAGAGAGAGAGGAAGCAGAGTTGTCTGAGAAAATTAAAT
  TGGAGCGCCAGCCGGAGCTTTGCGAGACCTCCCAGGCTCTGCCTTCCAAGCCTTTCTCATGTGGCCGGAG
  TGGAAAGGGACACAAAAGGAAAACCCCATTTGGAAATACAGAAAGAAAGACTCCTGTTAAAAAGCTGGCT
  CCTGAATTTTCAAAAGTCAAAACAAAAACTCCTAAGCACTCTCCCATTAAAGAGGAACCCTGTGGTTCCA
  TATCAGAAACTGTTTGTAAACGTGAATTGAGGAGCCAAGAAACCCCAGAAAAGCCCCGGTCTTCAGTGGA
  TACCCCACCAAGACTCTCGACTCCCCAAAAGGGACCCAGCACCCACCCCAAGGAGAAAGCCTTCTCAAGT
  GAGATGGAAGATTTGCCGTACCTTTCCACCACAGAAATGTATTTGTGTCGTTGGCACCAGCCTCCCCCAT
  CACCGTTACCATTACGGGAATCCTCTCCAAAGAAGGAGGAGACTGTAGCAAGGTGTCTGATGCCATCAAG
  TGTTGCAGGAGAAACTTCAGTCTTGGCTGTTCCTTCTTGGAGGGACCACTCAGTAGAGCCTCTAAGGGAC
  CCAAATCCTTCAGACATTTTGGAGAACCTGGATGACAGTGTATTTTCAAAGAGGCATGCAAAACTGGAGC
  TAGACGAGAAGAGGAGAAAACGATGGGATATTCAGAGGATCAGGGAACAAAGAATTTTACAGCGACTGCA
  GCTCAGAATGTATAAAAAGAAAGGAATTCAGGAATCTGAGCCTGAGGTTACCTCATTTTTCCCTGAGCCA
  GATGATGTTGAAAGTTTGCTGATTACCCCCTTCTTGCCTGTTGTAGCATTTGGACGGCCATTGCCAAAAT
  TAGCTCCACAGAACTTTGAACTACCCTGGTTGGATGAGCGAAGCCGTTGCAGGTTGGAGATCCAGAAGAA
  GCACACACCTCACCGGACCTGTAGGAAGTAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_028722
• Insert Size: 1851 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_028722.2, NP_082998.2
• RefSeq Size: 4358 bp
• RefSeq ORF: 1851 bp
• Locus ID: 74026
• UniProt ID: Q6PDM1
• Cytogenetics: 11 D
• Gene Summary: Component of histone acetyltransferase complex responsible for the majority of histone H4 acetylation at 'Lys-17' which is implicated in the formation of higher-order chromatin. structure (By similarity). Greatly enhances MSL2 E3 ubiquitin ligase activity, promoting monoubiquitination of histone H2B at 'Lys-35' (H2BK34Ub). This modification in turn stimulates histone H3 methylation at 'Lys-5' (H3K4me) and 'Lys-80' (H3K79me) and leads to gene activation, including that of HOXA9 and MEIS1. In the MSL complex, acts as a scaffold to tether MSL3 and KAT8 together for enzymatic activity regulation.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.