Klhl10 (NM_025727) Mouse Untagged Clone

CAT#: MC219627

Klhl10 (untagged) - Mouse kelch-like 10 (Drosophila) (Klhl10), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Klhl10
• Synonyms: 4921517C11Rik
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC219627 representing NM_025727
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGATGGAGAGCACCGCTGCCTCCACCCGGTTCCACCAGCCTCACATGGAAAGAAAGATGAGTGCAA
  TGACCTGTGAGATCTTCAACGAGCTCCGGCTTGAGGGCAAGCTCTGCGACGTGGTCATCAAAGTCAATGG
  CTTCGAGTTCAATGCCCACAAGAACATCCTCTGTAGCTGCAGCTCCTACTTTAGAGCTTTGTTTACAAGT
  GGCTGGAACAACACTGAGAAGAAGGTTTACAACATTCCTGGCATTTCCCCCGACATGATGAAGCTAATTA
  TTGAGTATGCGTACACTCGGACCGTGCCTATCACCCCTGACAATGTGGAGAAGCTGCTGGCCGCTGCTGA
  TCAGTTTAACATCATGGGCATCGTTAGGGGTTGCTGTGAGTTCCTCAAGTCGGAGCTGTGTCTGGATAAC
  TGTATCGGCATCTGCAAGTTCACGGACTACTACTACTGCCCCGAGCTGAGGCAGAAGGCCTACATGTTCA
  TCCTGCACAACTTCGAGGAGATGGTGAAAGTCTCGGCAGAGTTTCTGGAGCTCTCTGTCACTGAGCTCAA
  GGACATCATTGAGAAAGACGAACTCAACGTGAAGCAGGAGGACGCAGTATTCGAGGCCATTTTAAAGTGG
  ATTTCTCACGACCCCCAAAATAGGAAGCAACATATTTCAGTTTTACTTCCTAAGGTTCGCCTGGCCCTAA
  TGCATGCTGAGTACTTCATGAATAATGTTAAGATGAATGACTATGTCAAAGACAGTGAGGAATGTAAGCC
  AGTCATCATTAACGCCCTAAAGGCTATGTATGACCTGAACATGAACGGACCCTCCAATTCTGACTTCACC
  AACCCACTTACCAGGCCCCGCCTGCCCTATGCCATCCTATTCGCTATTGGTGGCTGGAGTGGCGGGAGCC
  CCACCAATGCCATTGAGGCATACGATGCTCGAGCAGACAGATGGGTGAATGTCACGTGTGAGGAAGAAAG
  TCCTCGAGCCTACCACGGGGCAGCCTACTTGAAAGGCTATGTTTATATCATCGGGGGGTTTGATAGCGTA
  GACTACTTCAATAGTGTTAAGCGTTTTGACCCTGTGAAGAAAACTTGGCACCAAGTGGCCCCTATGCACT
  CTAGACGTTGCTATGTCAGCGTGACAGTCCTCAGCAATTTCATCTATGCCATGGGAGGGTTTGATGGCTA
  CGTGCGTCTCAACACTGCTGAACGGTACGAGCCAGAGACCAACCAATGGACACTCATTGCCCCCATGCAT
  GAGCAGCGGAGCGATGCGAGCGCCACAACACTCTATGGGAAGGTCTACATATGTGGTGGGTTTAACGGAA
  ACGAATGCCTGTTTACAGCAGAAGTGTACAACACTGAGAGCAATCAATGGACAGTCATTGCACCCATGAG
  GAGCAGGAGGAGTGGAATAGGTGTAATTGCTTACGGGGAACATGTATACGCAGTGGGTGGCTTTGATGGA
  GCCAATCGACTGAGGAGTGCAGAAGCCTACAGCCCAGTGGCTAATACTTGGCGCACGATCCCCACTATGT
  TTAACCCTCGTAGCAATTTTGGGATCGAGGTAGTGGACGACCTCTTGTTTGTGGTGGGTGGCTTTAATGG
  CTTTACCACCACCTTTAACGTTGAGTGCTATGATGAAAAGACCGATGAGTGGTATGACGCTCATGACATG
  AGCATATACCGCAGTGCTCTGAGCTGCTGCGTGGTACCAGGGCTAGCCAATGTTGGGGAGTATGCAGCTA
  GACGGGACAACTTCACGGGATTAGCACTGCGAGATGAAGTAAAGTATTCCGCTTCGACAAGTACCCTACC
  TGTATGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_025727
• Insert Size: 1827 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_025727.3, NP_080003.1
• RefSeq Size: 2012 bp
• RefSeq ORF: 1827 bp
• Locus ID: 66720
• UniProt ID: Q9D5V2
• Cytogenetics: 11 D
• Gene Summary: May be a substrate-specific adapter of a CUL3-based E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins during spermatogenesis. Required for male fertility.[UniProtKB/Swiss-Prot Function]