Runx1t1 (NM_001111027) Mouse Untagged Clone

CAT#: MC219603

Runx1t1 (untagged) - Mouse runt-related transcription factor 1, translocated to, 1 (cyclin D-related) (Runx1t1), transcript variant 1, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Runx1t1
• Synonyms: Cbfa2t1h; ETO; MTG8
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC219603 representing NM_001111027
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGATATCTGTCAAAAGAAACACTTGGAGAGCACTGAGTGTAGTGATAGGTGACTGCCGGAAAAAGGCGA
  ACTTTGAATATTGTCAAGATCGTACCGAGAAGCACTCCACAATGCCAGACTCACCTGTGGATGTGAAGAC
  GCAGTCTAGGCTGACTCCCCCAGCAATGCCACCTCCTCCAACCACTCAAGGAGCTCCAAGAACCAGCTCA
  TTTACGCCAACGACATTAACGAATGGCACCAGCCATTCACCCACGGCCTTGAATGGCGCTCCCTCACCGC
  CTAATGGCTTTAGCAACGGGCCTTCTTCTTCCTCTTCCTCTTCTCTGGCTAATCAGCAGCTGCCCCCAGC
  CTGTGGTGCTAGGCAACTCAGCAAGCTGAAACGATTTCTTACTACCCTGCAGCAGTTTGGCAATGACATT
  TCACCAGAGATTGGAGAAAGAGTTCGCACCCTTGTACTGGGACTGGTGAACTCTACTTTGACAATTGAAG
  AATTTCATTCCAAACTGCAAGAAGCTACTAACTTCCCACTGAGACCTTTTGTCATCCCGTTTTTGAAGGC
  CAACCTGCCCCTGCTCCAGCGTGAACTCCTCCACTGCGCAAGGCTAGCCAAACAGAACCCTGCCCAGTAC
  CTCGCTCAGCACGAACAGCTGCTTCTGGATGCCAGCACCACCTCCCCTGTCGACTCCTCAGAGCTGCTTC
  TCGACGTGAACGAAAACGGGAAGAGGCGAACTCCAGACAGAACCAAAGAAAATGGCTTTGACAGAGAGCC
  TTTGCACTCAGAACATCCAAGCAAGCGACCATGCACTATTAGCCCAGGCCAGCGGTACAGTCCAAATAAT
  GGCTTATCCTACCAGCCAAATGGCCTGCCTCACCCCACCCCACCTCCACCTCAGCATTACCGATTGGATG
  ATATGGCCATTGCCCACCACTACAGGGACTCCTATCGACACCCCAGCCACAGGGACCTCAGGGACAGAAA
  TAGACCTATGGGGTTACATGGCACACGTCAAGAAGAAATGATTGACCACAGACTAACAGACAGAGAATGG
  GCAGAAGAGTGGAAACATCTCGATCATCTGTTAAACTGCATCATGGACATGGTGGAGAAGACAAGACGAT
  CCCTCACTGTACTAAGACGATGTCAGGAAGCGGACCGGGAAGAACTGAATTACTGGATACGGCGGTACAG
  TGATGCTGAGGACTTAAAAAAGGGTGGCAGCAGTAGCAGCAGCCACTCCAGGCAGCAGAGTCCTGTCAAT
  CCAGACCCAGTCGCATTAGATGCACATCGGGAATTCCTTCACAGGCCTGCGTCTGGATACGTGCCAGAGG
  AGATCTGGAAGAAAGCTGAGGAAGCTGTGAATGAGGTGAAGCGACAGGCAATGACGGAGCTGCAAAAAGC
  CGTGTCTGAGGCAGAGAGGAAAGCCCATGACATGATCACCACGGAGCGAGCCAAGATGGAACGCACGGTG
  GCTGAAGCGAAGAGGCAGGCAGCAGAAGATGCTCTAGCAGTCATCAACCAGCAGGAAGACTCCAGTGAGA
  GTTGCTGGAACTGTGGCCGGAAAGCAAGTGAGACGTGCAGCGGCTGTAACACGGCCCGATACTGTGGCTC
  TTTTTGCCAGCATAAAGACTGGGAGAAGCATCACCACATCTGTGGACAGACCCTGCAGGCCCCACAGCAG
  GGAGACACGCCCGCTGTCAGCTCCTCAGTCACACCCAGCAGTGGGGCTGGGAGCCCAATGGACACACCAC
  CAGCAGCCACTCCGAGGTCTACCACCCCGGGAACCCCCTCTACCATAGAGACGACGCCTCGCTAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001111027
• Insert Size: 1815 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001111027.1, NP_001104497.1
• RefSeq Size: 7518 bp
• RefSeq ORF: 1815 bp
• Locus ID: 12395
• Cytogenetics: 4 5.88 cM
• Gene Summary: Transcriptional corepressor which facilitates transcriptional repression via its association with DNA-binding transcription factors and recruitment of other corepressors and histone-modifying enzymes. Can repress the expression of MMP7 in a ZBTB33-dependent manner. Can repress transactivation mediated by TCF12 (By similarity). Acts as a negative regulator of adipogenesis (PubMed:23527555).[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The extent of this transcript is supported by transcript alignments.