Araf (NM_009703) Mouse Untagged Clone

CAT#: MC219601

Araf (untagged) - Mouse v-raf murine sarcoma 3611 viral oncogene homolog (Araf), transcript variant 1, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Araf
• Synonyms: 1200013E08Rik; A-Raf; Araf1; AW495444
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC219601 representing NM_009703
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAACCACCACGAGGCCCTCCTGTTAGTGGGGCTGAGCCATCTCGGGCAGTTGGCACTGTCAAAGTGT
  ACCTGCCTAACAAGCAACGCACAGTGGTGACTGTCCGGGAAGGCATGAGTGTCTATGACTCTTTGGACAA
  GGCCCTGAAGGTGCGGGGTCTCAACCAGGACTGCTGTGTGGTCTACAGGCTCATCAAAGGAAGAAAGACA
  GTCACTGCCTGGGACACAGCCATTGCGCCTCTGGATGGTGAGGAGCTCATTGTGGAGGTCCTAGAAGATG
  TGCCACTGACCATGCACAATTTTGTACGGAAGACATTCTTCAGCCTGGCCTTCTGTGACTTCTGCCTTAA
  GTTTCTGTTCCATGGATTCCGCTGCCAGACCTGTGGCTACAAGTTCCACCAGCATTGTTCCTCCAAGGTC
  CCCACCGTCTGCGTTGACATGAGTACCAACCGCCGACAGTTCTACCACAGCATCCAGGATCTGTCTGGAG
  GCTCCAGGCAGCAGGAGGCTCCCTCAAATCTCTCTGTGAATGAGCTGCTAACCCCCCAGGGTCCCAGCCC
  CTTTACCCAGCAACGTGACCAGGAGCACTTCTCATTCCCTGCCCCTGCCAATCCCCCACTGCAGCGCATC
  CGCTCCACATCTACTCCTAACGTCCACATGGTCAGCACCACAGCTCCCATGGACTCCAGCCTCATGCAGT
  TTACTGCTCAGAGCTTCAGCACCGATGCTGCTGGTAGAGGTGGTGATGGAGCCCCTCGGGGTAGCCCTAG
  CCCAGCTAGTGTGTCTTCAGGGAGGAAGTCCCCACATTCCAAGTTACCTTCAGAACAGCGGGAGCGGAAG
  TCCTTGGCAGATGAAAAGAAAAAAGTGAAGAACCTGGGGTACCGGGACTCAGGCTATTACTGGGAGGTGC
  CACCCAGTGAGGTACAGCTGTTGAAGAGGATCGGGACAGGCTCTTTTGGCACTGTGTTTCGGGGGCGTTG
  GCATGGCGATGTAGCTGTGAAAGTGCTCAAGGTGGCCCAACCTACCGCTGAGCAGGCCCAGGCCTTCAAG
  AATGAGATGCAGGTGCTCAGGAAGACACGGCATGTCAACATTTTGCTGTTCATGGGTTTCATGACTCGGC
  CGGGGTTTGCCATCATCACACAGTGGTGTGAGGGTTCCAGCCTCTACCACCACCTACATGTGGCTGACAC
  GCGCTTTGACATGGTCCAGCTCATTGATGTGGCCCGGCAGACTGCCCAGGGCATGGACTACCTCCACGCC
  AAGAACATCATTCATCGAGACCTAAAGTCCAACAATATCTTCTTACATGAGGGGCTCACAGTCAAGATTG
  GTGACTTCGGCCTGGCCACAGTGAAGACACGGTGGAGTGGGGCACAGCCCTTAGAGCAGCCCTCAGGGTC
  TGTGCTATGGATGGCAGCTGAGGTGATCCGTATGCAGGACCCGAACCCCTACAGCTTCCAGTCGGATGTC
  TATGCCTATGGTGTTGTGCTCTATGAGCTCATGACCGGCTCACTGCCCTACAGCCACATTGGCAGCCGTG
  ACCAGATCATCTTTATGGTGGGCCGTGGCTATCTGTCTCCGGACCTCAGCAAAATCTTCAGTAATTGCCC
  CAAAGCCATGAGGCGCCTGCTGACTGACTGCCTCAAGTTCCAGCGGGAGGAGCGGCCCCTATTTCCCCAG
  ATTCTGGCCACGATCGAGCTACTGCAGCGGTCACTCCCCAAGATTGAGCGGAGTGCCTCCGAACCCTCCT
  TGCACCGCACCCAGGCTGATGAGTTGCCTGCTTGCCTTCTCAGCGCAGCCCGTCTTGTGCCTTAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_009703
• Insert Size: 1815 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_009703.2, NP_033833.1
• RefSeq Size: 2405 bp
• RefSeq ORF: 1815 bp
• Locus ID: 11836
• UniProt ID: P04627
• Cytogenetics: X 16.3 cM
• Gene Summary: Involved in the transduction of mitogenic signals from the cell membrane to the nucleus. May also regulate the TOR signaling cascade (By similarity).[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1).