Ubqln4 (NM_033526) Mouse Untagged Clone

CAT#: MC219551

Ubqln4 (untagged) - Mouse ubiquilin 4 (Ubqln4), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Ubqln4
• Synonyms: A1u; A1Up; AI663987; CIP75; UBIN
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC219551 representing NM_033526
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGGAGCCGAGTGGGGCTGAGACGAGGCCCCAGATTCGGGTCACCGTCAAGACCCCCAAGGACAAGG
  AGGAGATCGTGATCTGCGATCAAGCCTCGGTCAAGGAGTTCAAGGAGGAGATCTCCCGGAGGTTTAAGGC
  TCAGCAGGATCAGCTGGTCCTGATCTTCGCAGGCAAGATCCTCAAGGATGGGGACACCCTGAGCCAGCAC
  GGCATCAAGGATGGGCTCACAGTCCACCTAGTCATCAAAACCCCTCAGAAGGCTCAAGATCCAGTGACTG
  CTGCTGCTTCTCCTCCATCCACTCCTGACTCTGCCTCTGCACCCTCCACCACGCCTGCCTCACCTGCCGC
  CGCCCCTGTCCAGCCCTGCAGCTCTGGCAATACAACTTCAGATGCTGGCAGTGGAGGGGGGCCCTCTCCA
  GTGGCTGCAGAGGGGCCCTCAAGTGCTACTGCATCGATCCTCTCTGGCTTCGGAGGCATCCTGGGACTGG
  GCAGCCTGGGCCTGGGCTCCGCCAACTTTATGGAGCTGCAGCAACAGATGCAGAGACAGCTCATGTCCAA
  TCCTGAGATGCTGTCGCAGATCATGGAGAACCCCTTGGTCCAGGATATGATGTCAAACCCTGATCTGATG
  CGCCACATGATCATGGCTAATCCCCAGATGCAGCAGCTGATGGAGAGGAACCCCGAGATCAGCCACATGC
  TCAACAACCCAGAGCTCATGAGGCAGACAATGGAGCTTGCTCGTAACCCAGCCATGATGCAAGAAATGAT
  GCGGAACCAGGACCGGGCCCTCAGCAACCTGGAAAGTGTCCCGGGAGGGTATAATGCCCTCCGCCGCATG
  TACACGGACATCCAGGAGCCCATGTTCACTGCTGCTCGGGAACAGTTTGGCAACAATCCCTTCTCTTCCC
  TGGCCGGGAACTCCGACAACTCATCCTCCCAACCTCTTCGGACTGAGAATCGAGAGCCCCTCCCTAACCC
  CTGGAGCCCCTCGCCCCCCACCTCCCAGGCCCCCGGGTCCGGTGGGGAGGGCACCGGAGGATCGGGGACC
  AGCCAGGTGCACCCGACAGTCTCGAACCCCTTTGGGATCAATGCGGCTAGCCTGGGGTCAGGGATGTTCA
  ACAGCCCAGAAATGCAGGCGCTCCTCCAGCAGATTTCTGAAAACCCCCAGCTGATGCAGAATGTCATCTC
  AGCCCCTTACATGCGCACCATGATGCAGACGCTTGCCCAGAACCCTGACTTTGCTGCTCAGATGATGGTG
  AATGTGCCGCTGTTCGCAGGGAATCCACAGCTTCAGGAGCAGCTCCGCCTGCAGCTCCCCGTGTTTCTGC
  AGCAGATGCAAAACCCAGAGTCTCTCTCCATCCTCACCAATCCCCGGGCCATGCAGGCCCTGCTGCAGAT
  CCAGCAGGGACTACAGACCCTCCAGACTGAAGCCCCTGGGCTGGTACCCAGCCTTGGCTCCTTTGGGACA
  CCCCGGACCTCGGTACCTCTGGCAGGCAGCAACTCTGGATCTTCGGCAGAGGCCCCCACTTCTTCCCCGG
  GCGTGCCAGCCACATCTCCTCCCAGTGCGGGTTCCAATGCCCAGCAACAACTCATGCAGCAGATGATCCA
  ACTTTTGTCCGGAAGTGGGAACTCACAGGTGCCGATGCCAGAAGTGAGATTTCAACAGCAGCTGGAACAA
  CTCAACTCCATGGGCTTTATTAACCGTGAGGCCAACCTACAGGCCCTGATCGCCACAGGAGGGGACATCA
  ATGCCGCTATTGAGAGACTCCTGGGCTCCCAGCTCTCCTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_033526
• Insert Size: 1791 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_033526.2, NP_277068.1
• RefSeq Size: 3372 bp
• RefSeq ORF: 1791 bp
• Locus ID: 94232
• UniProt ID: Q99NB8
• Cytogenetics: 3 F1
• Gene Summary: Regulator of protein degradation that mediates the proteasomal targeting of misfolded, mislocalized or accumulated proteins (By similarity). Acts by binding polyubiquitin chains of target proteins via its UBA domain and by interacting with subunits of the proteasome via its ubiquitin-like domain (By similarity). Key regulator of DNA repair that represses homologous recombination repair: in response to DNA damage, recruited to sites of DNA damage following phosphorylation by ATM and acts by binding and removing ubiquitinated MRE11 from damaged chromatin, leading to MRE11 degradation by the proteasome (By similarity). MRE11 degradation prevents homologous recombination repair, redirecting double-strand break repair toward non-homologous end joining (NHEJ) (By similarity). Specifically recognizes and binds mislocalized transmembrane-containing proteins and targets them to proteasomal degradation (By similarity). Collaborates with DESI1/POST in the export of ubiquitinated proteins from the nucleus to the cytoplasm (By similarity). Plays a role in the regulation of the proteasomal degradation of non-ubiquitinated GJA1 (PubMed:18079109, PubMed:20940304). Acts as an adapter protein that recruits UBQLN1 to the autophagy machinery (By similarity). Mediates the association of UBQLN1 with autophagosomes and the autophagy-related protein LC3 (MAP1LC3A/B/C) and may assist in the maturation of autophagosomes to autolysosomes by mediating autophagosome-lysosome fusion (By similarity).[UniProtKB/Swiss-Prot Function]