Ebf3 (NM_001113415) Mouse Untagged Clone

CAT#: MC219494

Ebf3 (untagged) - Mouse early B-cell factor 3 (Ebf3), transcript variant 1, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Ebf3
• Synonyms: 3110018A08Rik; mKIAA4201; O/E-2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC219494 representing NM_001113415
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTTTGGGATTCAGGAGAATATTCCGCGCGGGGGGACGACCATGAAGGAGGAGCCGCTGGGCAGCGGCA
  TGAACCCGGTGCGCTCGTGGATGCACACGGCAGGCGTGGTGGACGCCAACACGGCCGCCCAGAGCGGCGT
  GGGGCTGGCGCGGGCGCACTTCGAGAAGCAGCCTCCTTCCAACCTCCGGAAATCCAATTTCTTCCACTTC
  GTGCTGGCGCTCTACGACAGGCAGGGGCAGCCGGTGGAGATCGAAAGGACCGCTTTTGTGGACTTTGTGG
  AGAAAGAGAAAGAGCCGAACAACGAGAAAACCAACAACGGCATTCACTACAAGCTGCAGCTACTGTACAG
  CAATGGCGTCAGAACAGAGCAAGATCTGTATGTCCGCCTCATAGATTCAATGACCAAACAGGCCATCGTG
  TATGAAGGCCAGGACAAGAATCCGGAGATGTGCCGCGTGCTGCTGACCCACGAGATCATGTGCAGCCGGT
  GCTGTGACAAGAAGAGTTGTGGCAATAGAAACGAGACACCCTCAGACCCTGTAATCATTGACAGATTCTT
  TCTAAAGTTCTTCCTCAAGTGCAATCAGAACTGCCTGAAGAATGCGGGCAACCCTCGGGACATGCGGAGG
  TTCCAGGTCGTTGTATCGACAACAGTCAACGTGGACGGCCACGTGCTGGCCGTGTCTGACAACATGTTTG
  TGCACAACAATTCCAAACACGGGAGGCGGGCCCGCCGCCTAGACCCGTCAGAAGGTACGGCCCCTTCTTA
  TCTGGAAAATGCCACTCCGTGTATCAAGGCTATCAGCCCCAGTGAAGGCTGGACCACGGGGGGTGCCACT
  GTGATCATAATCGGGGACAACTTCTTTGACGGTCTACAAGTTGTGTTCGGAACTATGTTGGTGTGGAGTG
  AGCTGATAACCCCCCACGCCATCCGAGTGCAGACGCCGCCTCGACACATCCCTGGAGTTGTTGAAGTGAC
  CCTCTCCTACAAGTCTAAGCAGTTCTGCAAAGGCGCTCCTGGACGCTTTGTCTACACTGCCCTTAATGAG
  CCAACCATAGATTACGGCTTCCAGAGGTTGCAGAAAGTGATACCCAGACATCCAGGTGACCCCGAAAGGT
  TACCCAAGGAAGTGTTACTGAAGAGGGCAGCAGACCTTGTGGAAGCCTTGTATGGGATGCCTCACAATAA
  CCAGGAGATCATCCTGAAGAGAGCGGCCGACATTGCTGAGGCGTTGTACAGCGTTCCCCGCAATCACAAC
  CAGATCCCCACCCTGGGCAACACCCCAGCACACACTGGCATGATGGGTGTGAACTCCTTCAGCAGCCAGC
  TAGCCGTTAATGTGTCAGAGACCTCACAAGCCAATGACCAAGTCGGCTACAGTCGCAATACAAGCAGCGT
  GTCCCCGCGAGGCTACGTCCCCAGCAGTACTCCCCAGCAGTCCAATTACAACACAGTCAGCACTAGCATG
  AATGGATATGGAAGTGGCGCCATGGCCAATCTAGGGGTCCCTGGCTCGCCTGGATTTCTTAATGGCTCCT
  CCGCTAACTCTCCCTACGGCATAGTGCCGTCCAGCCCCACCATGGCAGCCTCTTCGGTCACCCTCCCTTC
  AAACTGTAGCAGCACACACGGCATTTTCTCATTCTCACCTGCCAATGTCATCTCCGCAGTGAAACAGAAG
  AGCGCTTTTGCGCCCGTGGTCCGGCCCCAAGCCTCGCCTCCTCCTTCCTGCACCAGCGCCAACGGGAACG
  GACTGCAAGCTATGTCTGGGCTGGTAGTCCCGCCCATGTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001113415
• Insert Size: 1791 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001113415.1, NP_001106886.1
• RefSeq Size: 5043 bp
• RefSeq ORF: 1791 bp
• Locus ID: 13593
• UniProt ID: O08791
• Cytogenetics: 7 F3- F4
• Gene Summary: Transcriptional activator (PubMed:9151732). Recognizes variations of the palindromic sequence 5'-ATTCCCNNGGGAATT-3' (By similarity).[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (1) encodes the longest isoform (1).