Tctn1 (NM_001039153) Mouse Untagged Clone

CAT#: MC219476

Tctn1 (untagged) - Mouse tectonic family member 1 (Tctn1), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Tctn1
• Synonyms: G730031O11Rik; Tect1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC219476 representing NM_001039153
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGGTCGCGGGGTCTCCCGCCGCTCCTGCTGGTGCTCCTAAACTGCTACACCTCTTCGAGCACCCAGG
  TCATTGCCATCCCGGCTGCGGCCACCCCAGCAGTGACGAAGGAGGATCTCAATTCCACCAAGGCCACCCC
  CACCACTCTGCAGCCCTCCCTGTCGCCTAGGACCCCGGGAACTCCCAGGGCACCGGAGCGCTCTGGCCCC
  AGGCCTACCCCGGTCACGGACGTTGCTGCTCTGTGTGTCTGCGACCTGCTTCCGGCACAGTGTGATGTCA
  ACTGCTGTTGCGACCCTGACTGCAGCCCTGCAGATTTCAGCATCTTCTCTGCCTGCTCAGTCCCAGTTGT
  CACGGGTGACAGGCAGTTTTGTAGTCAGAAGGCAGCCTTCTACTCAATGAATTTGACAGCAGACCCACCT
  CACCGTGACTTTAAACTCATTGATCAGATCAATCCTTCCGTCTTCTGCATCCATATTTCAAACTACAAGC
  CTGCACTGTCTTTTGCTAATCCAGAAGTGCCTGATGAAAACAATTTTGACAGACTGATGCAGACATCAGG
  TGGCTTTACACTGAGTGCCGAATCCGCTGTTCCTTCCACAGCCGCATCAGATGGCCCACAGCCCACTAAA
  TACGAGTATGGAGCCCCTCTGCAGACTGCAGGAGCATCTTCAGGGTCATTTCTGAAACTGCCCTCGCCTC
  TCACGTCATCTCTGTGTGCAGATCAGAACCCCGCAGCGTTCCTGGTGAGCCAGGCCTTCGAATGCAGCCG
  AAGGGTGGACATAGAACAGTGTGAAGGAATGGAAGCCCTCAGCATGGCTCATTACAGCAGCCCTGCCATT
  CTGAGGGTACCTAATTCAATGACACAGGTCTCCATCAAGATCCAGTCTGTAATGTATCGGTCTCTGAATC
  ACACACTGACGCAGTTAGAAGGCCATGGTGTCCTGCGGCCATCTCTGGTCAGCACTGGGCAAGACAGACT
  CTGCAGCAACGTTGTTCTCCAGGTGAAGTACAGTCTGCTGTACACAGCCACAGGCCAGATCCACGAAGCC
  GGCCTCTCCCTCGTTCTGGGGACACTGAGCAGCGCTGTGAGTCTCCTGCAGCAGAAGTTTGAGATTCACT
  TTATTCAGCATGGCACCAAGCCTGTTCCTCTCAGCGGCAACCCTGGTTACAGGGTGGGACTCCCGCTGGC
  TGCTGGCTTTCAGCCTCAGAAGGGATCTGGGATTATTCAGACAACGAACAGACAAGGCCAATTTACTATT
  CTTCGTAGCACGAGCCAGCAGGATTGCCTTGCCAGCGAGGGGCTTCGCACCCCTGTGCTATTTGGCTACA
  ATGTGCAGTCTGGCTGTCAGCTCAGGTTGACAGGCACCATCCCTTGTGGGCTTCTGGCACAGAAGGTGCA
  GGACCTGCTGAGAGGCCAGGCCTTCCCTGACTATGTGGCTGCCTTTGGGAACTCCCGGGCCCAGGATGTG
  CAGGACTGGGTGCCTGTGCACTTTGTCACCTACTCCTCCAACATGAAGGGCTCTTGCCAGCTTCCGGTGG
  CTTTGGCTATAGAAGTGAAATGGACTAAGTATGGGTCCCTGCTGAACCCCCAGGCCAGGATAGTAAATGT
  CACTGCCCAGCTCGTCTCAGTTCCAGAGCCCCTCCCAGGGCCTGAGAGGACCGTGGTCATTTCCACTGCA
  GTGACATTTGTGGATGTGTCAGCCCCTGCAGAGGCAGGCTTCCGAGCCCCACCCACCATCAACGCCAGGC
  TACCCTTCAGCTTCTTCTTCCCGTTTGTGTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001039153
• Insert Size: 1782 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001039153.3, NP_001034242.2
• RefSeq Size: 4087 bp
• RefSeq ORF: 1782 bp
• Locus ID: 654470
• UniProt ID: Q8BZ64
• Cytogenetics: 5 F
• Gene Summary: Component of the tectonic-like complex, a complex localized at the transition zone of primary cilia and acting as a barrier that prevents diffusion of transmembrane proteins between the cilia and plasma membranes. Regulator of Hedgehog (Hh), required for both activation and inhibition of the Hh pathway in the patterning of the neural tube. During neural tube development, it is required for formation of the most ventral cell types and for full Hh pathway activation. Functions in Hh signal transduction to fully activate the pathway in the presence of high Hh levels and to repress the pathway in the absence of Hh signals. Modulates Hh signal transduction downstream of SMO and RAB23.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (1) represents the longer transcript and encodes the supported protein. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.