Hpse2 (NM_001081257) Mouse Untagged Clone

CAT#: MC219475

Hpse2 (untagged) - Mouse heparanase 2 (Hpse2), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Hpse2
• Synonyms: Gm968; Hpa2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC219475 representing NM_001081257
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGGGTGCTCTGTGCTTTCCCTGAAGCCATGGCCTCCAGCAGCTCCCGCCCCCCCTCGTGCCTAGCCC
  TGGTGGCTCTCTTCTTGGCTCTGTTGCTCCATCTCTCCCTCTCCTTCCACGCTGGAAACAGGAGACCTTT
  GCCTGTAGACAGAGCTACAGGTTTGAAGGAAAAGACCCTGATTCTACTTGATGTGAGCACCAAGAACCCA
  GTCAGGACAGTCAATGAGAACTTCCTCTCTCTGCAGCTGGATCCCTCCATCATTCATGATGGCTGGCTCG
  ATTTCTTAAGCTCCAAGCGTCTGGTGACCTTGGCCCGGGGACTCTCGCCCGCCTTTCTGCGCTTCGGGGG
  CAAAAGGACGGATTTCCTGCAGTTCCAGAACCTGAGAAATCCGGCGAAAAGCCGCGGGGGCCCGGGCCCG
  GATTACTATCTCAAAAACTATGAGGATGACATTGTCCGGAGTGATGTTGCCTTGGACAAGCAGAAAGGCT
  GTAAGATTGCCCAGCACCCTGATGTCATGCTGGAGCTCCAGAGAGAGAAGGCATCGCAGATGCATCTGGT
  TCTTCTGAAGGAGCAATACTCCAATACTTACAGTAACCTCATATTAACAGCCAGGTCTCTAGACAAACTT
  TATAACTTTGCTGATTGCTCTGGACTCCACCTGATATTTGCTCTAAATGCACTGCGTCGTAATCCCAATA
  ACTCCTGGAACAGTTCTAGTGCCCTGAGCCTGTTGAAGTACAGTGCCAGCAAAAAGTACAACATTTCTTG
  GGAACTGGGTAATGAGCCAAATAACTATCGGAGCATCCATGGCCGGGCAGTAAATGGTAGCCAGTTGGGA
  AAGGATTACATCCAGCTGAAGAGCCTGCTCCAACCCATCCGGGTTTACTCCAGAGCCAGCCTGTACGGCC
  CCAACATCGGACGGCCCAGGAAGAACGTCATTGCTCTCCTAGATGGATTCATGAAGGTGGCAGGAAGCAC
  AGTGGATGCGGTTACCTGGCAACATTGCTACATTGATGGCCGGGTGGTCAAGGTGATGGACTTCCTGAAA
  ACTCGTCTGTTAGATACACTCTCTGACCAGATTAGGAAAATTCAGAAAGTGGTCAACACATACACTCCAG
  GAAAGAAGATTTGGCTGGAAGGCGTGGTGACCACCTCAGCTGGAGGCACAAACAATCTGTCGGATTCCTA
  CGCTGCAGGATTCTTATGGCTAAACACTTTAGGAATGTTGGCCAATCAGGGCATTGATGTCGTGATACGA
  CATTCATTTTTCGACCACGGATACAACCATCTGGTGGACCAGAATTTTAACCCATTACCAGACTATTGGC
  TTTCTCTGCTTTACAAGCGTCTGATTGGCCCCAAAGTCCTGGCAGTGCATGTAGCTGGGCTCCAGCGGAA
  GCCTCGGCCAGGAAGAGTCATCCGAGACAAACTAAGAATCTATGCTCACTGCACAAACCACCACAACCAC
  AACTATGTCCGTGGCTCTATCACACTTTTCATCATCAACCTACATCGATCAAGAAAGAAAATCAAACTGG
  CTGGGACTCTGAGGGACAAGCTCGTGCACCAGTATCTGCTGCAGCCCTATGGGCAGGAAGGCCTAAAGTC
  CAAGTCAGTGCAGCTCAATGGCCAGCCCTTAGTGATGGTGGACGACGGGACCCTTCCAGAACTGAAGCCC
  CGCCCGCTGCGGGCCGGCCGGACATTGGTCATCCCTCCAGTCACCATGGGCTTTTATGTGGTCAAGAATG
  TCAATGCTTTGGCCTGCCGTTATCGATAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001081257
• Insert Size: 1779 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001081257.2, NP_001074726.1
• RefSeq Size: 2175 bp
• RefSeq ORF: 1779 bp
• Locus ID: 545291
• UniProt ID: B2RY83
• Cytogenetics: 19 C3
• Gene Summary: Binds heparin and heparan sulfate with high affinity, but lacks heparanase activity. Inhibits HPSE, possibly by competing for its substrates (in vitro) (By similarity).[UniProtKB/Swiss-Prot Function]