C8b (NM_133882) Mouse Untagged Clone

CAT#: MC219449

C8b (untagged) - Mouse complement component 8, beta polypeptide (C8b), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: C8b
• Synonyms: 4930439B20Rik; AI595927
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC219449 representing NM_133882
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAAGATAGGGGCTCAGGTTTGGAGGGCACTGGCAAAGTCGTGTCTCCTCTGTGCTACTCTGGGATGTC
  TCCATTTCCCTGGTTCCAGAGGTGGGAAGCCCGATTTCTTTGAGACAAAGGCAGTCAATGGGAGCCTTGT
  CAAGAGCAGACCAGTACGGAGTGTGGCGGAAGCCCCAGCACCTATTGACTGTGAGCTATCCACCTGGTCT
  TCCTGGACTGCATGTGACCCCTGTCAGAAGAAAAGGTACAGACATACTTACTTGCTCCGGCCCTCTCAGT
  TCTATGGGGAATTGTGTGACTTGTCCGACAAGGAAGTTGAAGACTGTGTTACCAACCAACCCTGCAGGAG
  TCAAGTACGATGTGAAGGCTTTGTGTGTGCACAGACAGGGAGGTGTGTGAATCGCAGACTCCTTTGCAAT
  GGGGACAATGACTGTGGAGACCAATCAGATGAAGCCAACTGTAGGAGGATTTATAAAAACTGTCAACGGG
  AGATGGAGCAGTACTGGGCAATTGACAGGCTGGCCAGTGGGATAAATCTGTTCACAAACACTTTTGAGGG
  CCCAGTTCTCGATCACCGGTATTATGCTGGTGGATGCTCCCCCCACTACATCCTGGACACCAACTTTAGG
  AAGCCATACAACGTAGAAAGCTACACCCCACAGACCAAATGTGAATATGAATTCACACTTACAGAATATG
  AATCATATTCAGATTTTGAACGTCTAGTGATAGAAAAGAAAACCCACATGTTTAATTTCACGTCTGGTTT
  TAAAGTGGATGGCGTTATGGATCTTGGAATTAAAGTAGAAAGCAACGAAGGCAAAAATTATGTGACCAGA
  ACCAAACGCTTCGCCCACACTCAAAGCAAGTTCCTCCACGCGCGCTCTGTCCTTGAAGTGGCACATTACA
  AGCTGAAGTCTAGAAGCCTCATGCTCCATTATGAATTCCTTCAGAGGGTCAAGAGCCTGCCCCTGGAGTA
  TAGCTATGGGGAATACCGAGATCTTCTTCGTGACTTCGGGACCCATTTCATCACTGAAGCCGTACTTGGG
  GGCATCTACGAGTACACGCTTATCATGAACAAAGACGCCATGGAGCAAGGAGACTACACTCTTAGCCATG
  TTACTGCCTGTGCTGGAGGAAGTTTCGGTATTGGTGGTATGGTCTATAAAGTCTATGTCAAAGTGGGCGT
  TTCCGCAAAGAAATGCAGTGACATTATGAAAGAAATCAACGAAAGAAATAAGAGGAGCACCATGGTAGAA
  GACTTGGTAGTTCTTGTGAGAGGAGGGACAAGTGAGGATATCACTGCCTTGGCATACAAGGAGCTACCAA
  CCCCTGAACTGATGGAGGCATGGGGCGATGCTGTGAAGTACAACCCAGCAATCATTAAGATCAAGGCTGA
  GCCCCTGTATGAGCTTGTGACAGCCACAGACTTTGCATACTCCAGCACAGTGAAGCAGAACTTAAAGAAG
  GCACTAGAAGAATTCCAGAGTGAGGTCAGCTCCTGCCGCTGTGCTCCTTGCAGAGGGAATGGAGTCCCTG
  TCCTGAAAGGATCCCGCTGTGAGTGCATCTGCCCCGGTGGCTTCCAAGGCACAGCCTGTGAGGTTACCTA
  TCGGAAAGACATCCCCATAGATGGGAAGTGGAGTTGCTGGTCTGACTGGTCTGCATGCTCTGGAGGACAC
  AAAACAAGACACAGGCAGTGCAACAATCCAGCCCCTCACAAAGGAGGCAGCCCCTGTTCGGGTCCTGCTT
  CCGAAACACTCAACTGTTAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_133882
• Insert Size: 1770 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_133882.2, NP_598643.1
• RefSeq Size: 2239 bp
• RefSeq ORF: 1770 bp
• Locus ID: 110382
• UniProt ID: Q8BH35
• Cytogenetics: 4 48.61 cM
• Gene Summary: This gene encodes the beta subunit of complement component C8 that participates in the assembly of the complement membrane attack complex. The encoded preproprotein undergoes proteolytic processing to generate the beta subunit, which associates with the alpha and gamma subunits to form a trimeric complement component, C8. Alternative splicing results in multiple transcript variants encoding different isoforms that may undergo similar proteolytic processing. This gene is located adjacent to the gene encoding the alpha subunit. [provided by RefSeq, Oct 2015]
  Transcript Variant: This variant (1) represents the longer transcript and encodes the longer protein (isoform 1).