Fpgt (NM_029330) Mouse Untagged Clone

CAT#: MC219432

Fpgt (untagged) - Mouse fucose-1-phosphate guanylyltransferase (Fpgt), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Fpgt
• Synonyms: 1700016E03Rik
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC219432 representing NM_029330
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGTCTCTCCGCGAAGCCACCCTGCGGAAACTGCGCAGATTTTCTGAGCTGAGAGGCAAACCCGTGG
  CAGCTGGAGAATTCTGGGATGTGGTTGCAATAACAGCAGCTGATGAAAAGCAGGAGCTCGCTTACAAGCA
  ACAGTTGTCCGAGAAGCTGAAGAAAAGGGAATTGCCTCTTGGAGTTCAATACCATGTTTTTCCAGATCCT
  GCTGGGACCAAAATTGGAAATGGAGGATCAACACTTTGTTCCCTTGAGTGTTTGGAAAGCCTCTGTGGAG
  ACAAATGGAATTCTCTGAAGGTCCTGCTAATCCACTCTGGTGGCTACAGCCAACGCCTTCCCAATGCGAG
  TGCTTTAGGAAAGATCTTCACAGCCTTACCACTTGGTGAACCCATTTATCAGATGTTGGAGTTAAAACTA
  GCCATGTACGTGGATTTCCCCTCAAACATGAGGCCTGGAGTCTTGGTCACCTGTGCAGATGATATCGAAC
  TCTACAGTGTTGGGGACAGTGAGTACATTGCCTTTGACCAGCCTGGCTTTACTGCCTTAGCCCATCCGTC
  TAGTCTGGCTGTAGGCACTACTCATGGAGTATTTGTCTTGCACTCTGACAGTTCCTTACAACATGGTGAC
  CTTGAGTACAGGCAATGCTACCAATTCCTCCACAAGCCCACCATTGAAAACATGCACCGCTTTAATGCTG
  TGCATAGACAACGAAGCTTTGGTCAACAGAACTTGTCTGGAGGTGACACTGACTGTCTTCCATTGCACAC
  TGAGTATGTCTACACAGATAGCCTGTTTTACATGGATCACAAATCAGCCAAAAAGTTACTTGATTTCTAT
  AAAAGTGAAGGCCCACTGAACTGTGAAATAGATGCCTATGGAGACTTTCTTCAGGCACTGGGGCCTGGAG
  CAACTGCAGAGTACACCAGGAACACATCTCATGTCACTAAAGAAGAGTCCCAGTTGTTGGACATGAGGCA
  GAAAATATTCCACCTCCTCAAGGGAACACCACTGAATGTTGTTGTTCTTAATAACTCCAGATTTTATCAC
  ATTGGAACACTGCAAGAGTATCTGCTTCATTTCACCTCTGATAGTGCATTAAAGACGGAGCTGGGCTTAC
  AATCCATAGCTTTCAGTGTCTCTCCAAGTGTTCCTGAGCGCTCCAGTGGAACAGCCTGTGTCATTCACAG
  TATAGTGGATTCAGGATGCTGTGTGGCCCCTGGCTCAGTGGTAGAGTATTCTAGATTGGGGCCTGAGGTG
  TCCATCGGGGAAAACTGCATTATCAGCAGTTCTGTCATAGCAAAAACTGTTGTGCCAGCATATTCTTTTT
  TGTGTTCTTTAAGTGTGAAGATAAATGGACACTTAAAATATTCTACTATGGTGTTTGGCATGCAAGACAA
  CTTGAAGAACAGTGTTAAAACACTGGAAGACATAAAGGCACTTCAGTTCTTTGGAGTCTGTTTTCTGTCT
  TGTTTAGACATTTGGAATCTTAAAGCTACAGAGAAACTATTCTCTGGAAATAAGATGAATCTGAGCCTGT
  GGACTGCATGCATTTTCCCTGTCTGTTCATCTCTGAGTGAGTCGGCTACAGCATCCCTTGGGATGTTAAG
  CGCTGTAAGGAACCATTCACCATTCAACCTAAGTGACTTTAACCTTTTGTCCATCCAGGAAATGCTTGTC
  TACAAAGATGTACAAGACATGCTAGCTTATAGGGAACACATTTTTCTAGAAATTAGTTCAAATAAAAATC
  AATCTGATTTAGAGAAATCTTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_029330
• Insert Size: 1773 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_029330.2, NP_083606.2
• RefSeq Size: 3526 bp
• RefSeq ORF: 1773 bp
• Locus ID: 75540
• UniProt ID: G5E8F4
• Cytogenetics: 3 H4
• Gene Summary: Catalyzes the formation of GDP-L-fucose from GTP and L-fucose-1-phosphate (PubMed:14686921). Functions as a salvage pathway to reutilize L-fucose arising from the turnover of glycoproteins and glycolipids (Probable).[UniProtKB/Swiss-Prot Function]