Mars2 (NM_175439) Mouse Untagged Clone

CAT#: MC219335

Mars2 (untagged) - Mouse methionine-tRNA synthetase 2 (mitochondrial) (Mars2), nuclear gene encoding mitochondrial protein, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Mars2
• Synonyms: C730026E21Rik; MetRS
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC219335 representing NM_175439
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCTGCGGCAGTGTGCTCGCTGGGTGTTGACGCGCACCCGGTTCGGCCGCGGCTGTCGCCGCTACGGTT
  CGTGCTCGCCGAGCGCTAGTGGCGACGCCGGGGAAGCGCGGGCCTACTTCACCACACCTATCTTCTACGT
  GAACGCCGCGCCGCACATCGGGCACCTGTACTCGGCGCTACTGGCCGACGCCCTGTGCCGCCACCGTCGT
  CTCCGAGTTCCCGGCTCTGCGTCCACGCGGTTTTCCACCGGCACGGATGAGCACGGCCTGAAGATCCAGC
  AGGCGGCAGCCACCGCCGGCTTGGCCCCGATCGAGCTGTGCGATCGAGTGTCTGCCCAGTTTTTGCAGCT
  TTTCCGGGAGGCTGACATCTCTTCCACGGACTTCATCCGCACCACGGAGGCTCGGCACCGAGTAGCGGTG
  CAGCATTTCTGGGGTGTGCTGGAGGCCCGGGGTCTGCTTTACAAAGGGATCTATGAAGGGTGGTATTGTG
  CCTCGGATGAGTGCTTCTTGCCGGAGGCCAAGGTTACTCGGCAGGTGGGTCCGTCCGGGGATCCCTGTCC
  CGTGTCTCTCGAGAGCGGACATCCTGTCTCCTGGACGAAGGAAGAAAATTACATTTTCAAGCTTTCTCAG
  TTTCGAGAACCACTCCAGCGGTGGCTTGGAAACAATCCTCAGGCAATCACACCGGAACCGTTCCACCAGG
  CAGTTCTTCAGTGGCTGGAGGAGGAGCTGCCCGACTTGTCCGTTTCTCGAAGGAGTAGCCATCTGCACTG
  GGGCATACCAGTTCCCGGGGACGACTCGCAGACCATCTACGTGTGGCTGGATGCCCTGGTCAACTACCTC
  ACTGTAGTTGGCTACCCAGATGCAGATTTCAAGTCTTGGTGGCCAGCCACCTCTCATATCATAGGTAAGG
  ACATTCTCAAATTTCATGCTATTTATTGGCCTGCCCTTCTCCTAGGGGCAGGATTGAGGCCACCACATCG
  CATCTATGTCCACTCGCACTGGACGGTGAGTGGCCAAAAAATGTCCAAAAGCTTGGGCAACGTGGTGGAT
  CCCAGGACTTGCCTTGATCGCTATACTGTGGATGGCTTTCGCTACTTTCTCCTTCGGCAGGGCGTTCCCA
  ATTGGGACTGCGATTACTATGATGAAAAGGTGGTTAAATTGCTGGACTCCGAGCTGGCAGATGCCTTAGG
  AGGGCTTTTAAACCGGTGCACTGCCTACAGAATAAATCCTTCTGGAACCTACCCATCTTTCTGTGCTGCC
  TGTTTCCCCAGTGAGCCAGGGTTAACGGGGCCGTCAGTTCGTGTGCAGGCAGAAGACTATGCGCTTGTAA
  CTGCAGTGGCCACTTTACCCAAACTGGTAGCGGGCTATTATAATGACTTTCAGATCTATAAGGCTCTGGA
  GGCGGTATCCAGCTGTGTTCGGCAAACTAATGGTTTCGTCCAAAGGCACGCACCATGGAAATTGAACTGG
  GAGAGCCCTGAGGATGCTCCCTGGCTGGGTACCGTGCTTCACGTAGCCTTGGAGTGCTTGCGAGTGTTTG
  GAACTTTGCTTCAGCCTGTTACTCCAAACTTAGCAGATAAACTGCTGTCAAGACTGGGAGTCTCTACCAC
  AGAGAGAGGCCTTGGAGAGCTCTACTTCCTACCTCGATTCTATGGACACCCATGCCCCTTTGAGGGGAGA
  AAGTTGGGACCTGACACTGGGCTTTTGTTTCCTAGACTGGACCAATCTAGGACTCGGCTAGTGAAAGCCC
  ACAGGACCTAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_175439
• Insert Size: 1761 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_175439.3, NP_780648.1
• RefSeq Size: 2882 bp
• RefSeq ORF: 1761 bp
• Locus ID: 212679
• UniProt ID: Q499X9
• Cytogenetics: 1 C1.2