Mars2 (NM_175439) Mouse Tagged ORF Clone

CAT#: MG209149

Mars2 (tGFP-tagged) - Mouse methionine-tRNA synthetase 2 (mitochondrial) (Mars2)

Product Images

Circular map for MG209149

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Mars2
• Synonyms: C730026E21Rik; MetRS
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG209149 representing NM_175439
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCTGCGGCAGTGTGCTCGCTGGGTGTTGACGCGCACCCGGTTCGGCCGCGGCTGTCGCCGCTACGGTT
  CGTGCTCGCCGAGCGCTAGTGGCGACGCCGGGGAAGCGCGGGCCTACTTCACCACACCTATCTTCTACGT
  GAACGCCGCGCCGCACATCGGGCACCTGTACTCGGCGCTACTGGCCGACGCCCTGTGCCGCCACCGTCGT
  CTCCGAGTTCCCGGCTCTGCGTCCACGCGGTTTTCCACCGGCACGGATGAGCACGGCCTGAAGATCCAGC
  AGGCGGCAGCCACCGCCGGCTTGGCCCCGATCGAGCTGTGCGATCGAGTGTCTGCCCAGTTTTTGCAGCT
  TTTCCGGGAGGCTGACATCTCTTCCACGGACTTCATCCGCACCACGGAGGCTCGGCACCGAGTAGCGGTG
  CAGCATTTCTGGGGTGTGCTGGAGGCCCGGGGTCTGCTTTACAAAGGGATCTATGAAGGGTGGTATTGTG
  CCTCGGATGAGTGCTTCTTGCCGGAGGCCAAGGTTACTCGGCAGGTGGGTCCGTCCGGGGATCCCTGTCC
  CGTGTCTCTCGAGAGCGGACATCCTGTCTCCTGGACGAAGGAAGAAAATTACATTTTCAAGCTTTCTCAG
  TTTCGAGAACCACTCCAGCGGTGGCTTGGAAACAATCCTCAGGCAATCACACCGGAACCGTTCCACCAGG
  CAGTTCTTCAGTGGCTGGAGGAGGAGCTGCCCGACTTGTCCGTTTCTCGAAGGAGTAGCCATCTGCACTG
  GGGCATACCAGTTCCCGGGGACGACTCGCAGACCATCTACGTGTGGCTGGATGCCCTGGTCAACTACCTC
  ACTGTAGTTGGCTACCCAGATGCAGATTTCAAGTCTTGGTGGCCAGCCACCTCTCATATCATAGGTAAGG
  ACATTTTCAAATTTCATGCTATTTATTGGCCTGCCCTTCTCCTAGGGGCAGGATTGAGGCCACCACATCG
  CATCTATGTCCACTCGCACTGGACGGTGAGTGGCCAAAAAATGTCCAAAAGCTTGGGCAACGTGGTGGAT
  CCCAGGACTTGCCTTGATCGCTATACTGTGGATGGCTTTCGCTACTTTCTCCTTCGGCAGGGCGTTCCCA
  ATTGGGACTGCGATTACTATGATGAAAAGGTGGTTAAATTGCTGGACTCCGAGCTGGCAGATGCCTTAGG
  AGGGCTTTTAAACCGGTGCACTGCCTACAGAATAAATCCTTCTGGAACCTACCCATCTTTCTGTGCTGCC
  TGTTTCCCCAGTGAGCCAGGGTTAACGGGGCCGTCAGTTCGTGTGCAGGCAGAAGACTATGCGCTTGTAA
  CTGCAGTGGCCACTTTACCCAAACTGGTAGCGGGCTATTATAATGACTTTCAGATCTATAAGGCTCTGGA
  GGCGGTATCCAGCTGTGTTCGGCAAACTAATGGTTTCGTCCAAAGGCACGCACCATGGAAATTGAACTGG
  GAGAGCCCTGAGGATGCTCCCTGGCTGGGTACCGTGCTTCACGTAGCCTTGGAGTGCTTGCGAGTGTTTG
  GAACTTTGCTTCAGCCTGTTACTCCAAACTTAGCAGATAAACTGCTGTCAAGACTGGGAGTCTCTACCAC
  AGAGAGAGGCCTTGGAGAGCTCTACTTCCTACCTCGATTCTATGGACACCCATGCCCCTTTGAGGGGAGA
  AAGTTGGGACCTGACACTGGGCTTTTGTTTCCTAGACTGGACCAATCTAGGACTCGGCTAGTGAAAGCCC
  ACAGGACC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG209149 representing NM_175439
  Red=Cloning site Green=Tags(s)
  
  MLRQCARWVLTRTRFGRGCRRYGSCSPSASGDAGEARAYFTTPIFYVNAAPHIGHLYSALLADALCRHRR
  LRVPGSASTRFSTGTDEHGLKIQQAAATAGLAPIELCDRVSAQFLQLFREADISSTDFIRTTEARHRVAV
  QHFWGVLEARGLLYKGIYEGWYCASDECFLPEAKVTRQVGPSGDPCPVSLESGHPVSWTKEENYIFKLSQ
  FREPLQRWLGNNPQAITPEPFHQAVLQWLEEELPDLSVSRRSSHLHWGIPVPGDDSQTIYVWLDALVNYL
  TVVGYPDADFKSWWPATSHIIGKDIFKFHAIYWPALLLGAGLRPPHRIYVHSHWTVSGQKMSKSLGNVVD
  PRTCLDRYTVDGFRYFLLRQGVPNWDCDYYDEKVVKLLDSELADALGGLLNRCTAYRINPSGTYPSFCAA
  CFPSEPGLTGPSVRVQAEDYALVTAVATLPKLVAGYYNDFQIYKALEAVSSCVRQTNGFVQRHAPWKLNW
  ESPEDAPWLGTVLHVALECLRVFGTLLQPVTPNLADKLLSRLGVSTTERGLGELYFLPRFYGHPCPFEGR
  KLGPDTGLLFPRLDQSRTRLVKAHRT
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_175439
• ORF Size: 1758 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_175439.3, NP_780648.1
• RefSeq Size: 2882 bp
• RefSeq ORF: 1761 bp
• Locus ID: 212679
• UniProt ID: Q499X9
• Cytogenetics: 1 C1.2