Smox (NM_145533) Mouse Untagged Clone

CAT#: MC219013

Smox (untagged) - Mouse spermine oxidase (Smox), transcript variant 2, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Smox
• Synonyms: B130066H01Rik; PAO; PAOh1; SMO
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC219013 representing NM_145533
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCAAAGTTGTGAATCCAGTGGCGACAGTGCGGATGACCCTCTCAGTCGTGGCCTACGGAGAAGGGGAC
  AGCCTCGTGTGGTGGTGATCGGTGCTGGCTTGGCTGGCCTGGCTGCAGCTAGAGCCCTTCTGGAGCAGGG
  CTTCACGGATGTCACTGTGCTTGAGGCTTCCAGCCACATTGGGGGCCGTGTGCAGAGTGTGAGGCTTGGA
  GACACCACCTTTGAGCTGGGAGCCACCTGGATCCATGGATCCCACGGGAATCCTATCTATCAACTAGCAG
  AAGCCAATGGCCTTTTGGAAGAGACAACAGATGGGGAGCGCAGTGTGGGCCGCATCAGCCTTTACTCCAA
  GAATGGCGTGGCCTGCTACCTTACCAACCGTGGCTGCCGCATCCCCAAGGACGTGGTTGAGGAATTCAGC
  GATTTATACAACGAGGTCTATAACATGACCCAGGAGTTCTTCCGGCATGGTAAACCAGTCAATGCCGAGA
  GTCAGAACAGCGTCGGGGTGTTCACCCGGGAGAAGGTGCGGAATCGCATCAGGGATGACCCTGACGACAC
  AGAGGCCACCAAGCGCCTGAAGCTCGCCATGATCCAGCAGTACCTGAAGGTGGAGAGCTGTGAGAGCAGC
  TCCCACAGCATAGATGAGGTGTCCCTGAGCGCCTTTGGAGAATGGACGGAGATCCCAGGCGCCCATCACA
  TCATCCCCTCGGGCTTCATGCGAGTTGTGGAGCTGCTGGCTGAGGGCATTCCTCCACATGTCATCCAGTT
  GGGGAAGCCGGTCCGTTGCATCCACTGGGACCAGGCCTCGGCTCACCCCCGGGGTCCTGAGATCGAGCCC
  CGTGGTGAGGGTGATCACAATCACGACACTGGGGAGGGTGGCCAGAGTGGAGAGAATCCGCAGCAGGGGA
  GGTGGGACGAGGATGAGCCGTGGCCTGTAGTCGTGGAGTGCGAGGATTGCGAGGTGATCCCAGCGGACCA
  CGTGATTGTGACCGTTTCGCTGGGCGTGCTCAAGAGGCAGTACACCAGTTTCTTTAGGCCATGCCTGCCC
  ACGGAGAAGGTGGCCGCCATCCACCGCCTGGGCATTGGTACCACTGACAAGATCTTCCTTGAATTTGAGG
  AGCCCTTTTGGGGCCCCGAGTGCAACAGCCTGCAGTTCGTGTGGGAGGATGAGGCAGAGAGCTGTACCCT
  CACCTACCCACCTGAGCTCTGGTACCGCAAGATCTGTGGCTTCGATGTCCTTTATCCGCCAGAGCGCTAT
  GGCCATGTGCTGAGTGGCTGGATCTGTGGGGAGGAGGCTCTTGTCATGGAGAGGTGCGATGACGAGGCTG
  TAGCTGAGATCTGCACAGAGATGCTTCGACAGTTCACAGGGAACCCCAATATACCAAAACCTAGGCGAAT
  CCTGCGCTCAGCCTGGGGCAGCAACCCATACTTCCGGGGTTCCTATTCCTACACACAGGTGGGCTCAAGT
  GGGGCGGATGTGGAGAAGCTAGCCAAGCCCCTGCCCTACACAGAGAGCTCCAAGACAGCGCCCATGCAGG
  TGCTCTTCTCCGGGGAGGCCACACACCGCAAGTACTACTCCACCACCCACGGTGCTCTGCTCTCTGGCCA
  GCGCGAGGCCGCCCGGCTCATCGAGATGTACCGAGACCTCTTCCAGCAGGGGCCCTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_145533
• Insert Size: 1668 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_145533.2, NP_663508.1
• RefSeq Size: 2190 bp
• RefSeq ORF: 1668 bp
• Locus ID: 228608
• UniProt ID: Q99K82
• Cytogenetics: 2 F1
• Gene Summary: Flavoenzyme which catalyzes the oxidation of spermine to spermidine. Can also use N(1)-acetylspermine and spermidine as substrates, with different affinity depending on the isoform (isozyme) and on the experimental conditions. Plays an important role in the regulation of polyamine intracellular concentration and has the potential to act as a determinant of cellular sensitivity to the antitumor polyamine analogs. May contribute to beta-alanine production via aldehyde dehydrogenase conversion of 3-amino-propanal.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (2) lacks an alternate, in-frame, exon, compared to variant 1. The resulting protein (isoform b) is shorter when it is compared to isoform a. This transcript and protein have also been called 'polyamine oxidase-l'.