Trim47 (NM_172570) Mouse Untagged Clone

CAT#: MC219002

Trim47 (untagged) - Mouse tripartite motif-containing 47 (Trim47), transcript variant 2, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Trim47
• Synonyms: 2210023F24Rik
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC219002 representing NM_172570
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGACGGCAGCGGGCCTTTCAGCTGCCCCATCTGCCTGGAACCGCTCCGGGAGCCGGTGACACTACCCT
  GCGGCCACAACTTCTGCCTCGCTTGCCTGGGCGCTCTCTGGCCGCACCGGAGCGCGGGCGGCACCGGTGG
  TTCCGGAGGTCCAGCTCGCTGTCCACTTTGTCAGGAGCCTTTCCCCGACGGCCTGCAGCTCCGCAAGAAC
  CACACGCTGTCCGAGTTGCTGCAGCTCCGCCAGGGCTCGGTCCCGGGACCCATGTCCGCCCCGGCTTCAG
  GATCGACCCGGGGCGCCACGCCCGAGCCCTCCGCGCCCAGCGCACCACCTCCGGCTCCGGAACCATCGGC
  TCCCTGCGCGCCGGAGCAGTGGCCCGCGGGTGAGGAGCCGGTGCGCTGCGACGCGTGCCCCGAGGGCGCC
  GCCCTGCCCGCCGCGCTCTCTTGCCTCTCCTGCCTCGCCTCCTTCTGCTCCGCGCATCTGGCCCCCCACG
  AACGCAGCCCCGCCCTGCGCGGCCACCGCCTGGTGCCCCCGCTGCGCCGACTTGAGGAGAGCCTGTGTCC
  GCGCCACCTGCGGCCTCTGGAGCGCTACTGTCGCGTGGAGCGTGTGTGCCTTTGCGAGGCCTGTGCCACT
  CAGGACCATCGCGGCCACGAGCTCGTGCCGCTGGAGCAGGAGCGCGCGCTTCAGGAGGTGGAGCAGTCCA
  AAGTCCTGAGCGCTGCGGAGGACCGCATGGATGAACTGGGTGCCGGCATCGCACAGTCCCGCCGTACTGT
  GGCCCTCATCAAGAGTGCAGCTGTGGCAGAGCGGGAGAGGGTGAGCCAGATGTTTGCCGAGGCCACAGCC
  ACCCTTCAGAGTTTCCAGAACGAGGTGATGGGGTTCATCGAAGAGGGAGAGGCCACAATGTTGGGCCGGT
  CCCAGGGTGACCTGCGTAGACAAGAGGAACAGCGCAGCCGCCTAAGCAAGGCACGGCACAACCTCGGTCA
  GGTTCCAGAGGCGGATTCAGTCAGCTTTCTCCAGGAGCTCCTGGCACTACGGCTGGCCCTGGAGGAGGGG
  TGCGGCCCCGGGCCTGGGCCCCCCAGGGAACTCAGCTTCACCAAGTCCTCCCAGGTGGTCAAAGCGGTGA
  GAGACACCCTCATCTCAGCCTGTGCCAGCCAGTGGGAGCAGCTGCGGGGGCTGGGCAGCAATGAGGATGG
  GCTACAGAAACTCGGCTCAGAAGATGTGGAGTCCCAGGACCCTGACAGTACCAGCCTGCTGGAGAGTGAG
  GCCCCCAGGGATTACTTCCTCAAGTTTGCCTACATCGTGGACCTGGACAGCGACACCGCGGACAAGTTCC
  TGCAGCTTTTTGGAACTAAAGGTGTCAAGAGAGTACTGTGTCCCATCAACTACCCGGAGTCACCCACCCG
  CTTCACACACTGCGAGCAGGTGCTAGGAGAGGGCGCCCTGGACCGGGGCACCTACTACTGGGAGGTGGAG
  ATCATCGAAGGATGGGTCAGTGTGGGAGTCATGGCCGAGGGCTTCTCCCCGCAAGAGCCCTATGACCGGG
  GCCGGCTGGGCCGCAATGCACACTCATGCTGTCTGCAGTGGAATGGGCGTGGCTTCTCAGTCTGGTTCTG
  CGGGCTGGAGGCCCCGCTACCCCATGCCTTTTCGCCTACAGTCGGGGTCTGCCTAGAGTATGCAGACCAT
  GCCCTGGCCTTCTATGCTGTCCGAGACGGAAAGCTGAGCCTTCTTCGGAGGCTCAAAGCCTCCCGGCCTC
  GCCGCAGCGGTGCTCTGGCCTCCCCCACTGACCCTTTTCAGAGTCGCCTGGACAGCCATTTTTCCGGGCT
  CTTCAACCACAGGCTCAAGCCTGCCTTCTTCCTGGAGAGTGTGGACGCCCATCTGCAGATTGGACCTCTC
  AAGAAATCGTGCATAACCGTGCTGAAGAGGAGGTGA
  
  AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC
  TGGATTACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-RsrII
• ACCN: NM_172570
• Insert Size: 1926 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_172570.5, NP_766158.3
• RefSeq Size: 2192 bp
• RefSeq ORF: 1926 bp
• Locus ID: 217333
• UniProt ID: Q8C0E3
• Cytogenetics: 11 E2
• Gene Summary: E3 ubiquitin-protein ligase that mediates the ubiquitination and proteasomal degradation of CYLD.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (2) uses an alternate in-frame splice site in the 3' coding region, compared to variant 1. This results in a shorter protein (isoform 2), compared to isoform 1.