Trim23 (NM_030731) Mouse Untagged Clone

CAT#: MC218991

Trim23 (untagged) - Mouse tripartite motif-containing 23 (Trim23), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Trim23
• Synonyms: 6330516O20Rik; AI450195; Arfd1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC218991 representing NM_030731
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTTTCTGTTTTATATACAGGTTCTGGAGTGTGGGGTTTGTGAAGATGTCTTCTCTTTGCAAGGAGATA
  AAGTTCCTCGCCTCCTCCTCTGTGGCCACACAGTCTGTCATGACTGTCTTACTCGCTTGCCTCTGCATGG
  AAGAGCAATACGTTGCCCATTTGATCGACAAGTAACAGACCTAGGAGACTCAGGTGTATGGGGTTTGAAA
  AAGAACTTTGCTTTACTGGAGCTTTTAGAACGTTTGCAAAATGGGCACATTGGTCAGTATGGAGCTGCAG
  AAGAGGCCATTGGGACATCTGGAGAGAGCATCATTCGCTGTGATGAAGATGAAGCTCACGTTGCATCTGT
  ATATTGCACTGTGTGTGCAACTCACTTGTGCTCAGACTGTTCTCAAGTTACTCATTCTACAAAGACATTA
  GCAAAGCATAGGCGTGTGCCTCTAGCTGATAAACCTCATGAGAAAACCATGTGCTGTCAGCACCAGGTGC
  ATGCCATTGAGTTTGTTTGCTTGGAAGAAGGTTGTCAAACTAGTCCACTCATGTGCTGTGTCTGCAAAGA
  ATATGGAAAGCACCAAGGTCACAAGCATTCAGTACTGGAACCAGAAGCTAATCAGATCCGAGCATCAATT
  TTAGACATGGCTCACTGCATACGGACCTTCACTGAGGAAATCTCAGATTATTCCAGAAAATTAGTTGGGA
  TTGTTCAGCACATTGAAGGAGGAGAACAAATAGTAGAAGATGGAATTGGAATGGCTCACACAGAACATGT
  CCCAGGTACTGCAGAGAATGCCCGATCATGTGTCAGAGCTTATTTTTCTGATTTACATGAAACTCTTTGT
  CGTCAAGAAGAAATGGCTCTAAGTGTTGTTGATGCCCATGTTCGAGAAAAACTGATTTGGCTTAGGCAGC
  AACAAGAGGATATGACTATTCTCTTGTCCCAGGTCTCAACAGCCTGTCTCCACTGTGAAAAAACTCTGCA
  GCAGGATGATTGCAGAGTTGTCTTGGCAAAACAAGAAATCACAAGATTACTAGAAACACTGCAGAAACAG
  CAGCAGCAGTTTACAGAGGTTGCAGATCATATTCAGTTGGATGCCAGTATTCCAGTCACTTTTACAAAGG
  ACAATAGAGTTCATATTGGACCCAAAATGGAAATCCGAGTAGTCACATTAGGATTAGATGGTGCTGGAAA
  AACTACCATTTTGTTCAAGTTAAAACAAGATGAATTTATGCAACCTATTCCAACAATTGGTTTTAATGTG
  GAAACTGTGGAATACAAAAATCTAAAATTCACCATTTGGGATGTGGGTGGAAAACACAAATTAAGACCAT
  TGTGGAAACATTATTACCTCAATACACAAGCTGTTGTATTTGTTGTTGATAGTAGTCATAGAGACAGAAT
  TAGTGAAGCACACAGTGAACTTGCAAAGTTGTTAACGGAAAAAGAACTCCGAGATGCCTTACTCTTGATT
  TTTGCTAACAAACAGGATGTAGCTGGAGCACTTTCAGTAGAAGAAATCACTGAACTTCTCAGTCTCCATA
  AACTATGCTGTGGAAGGAGTTGGTATATTCAGGGCTGTGATGCTCGAAGTGGAATGGGGCTCTACGAAGG
  GTTGGACTGGCTGTCCCGGCAACTTGTGGCTGCTGGAGTGTTGGATGTTGCTTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_030731
• Insert Size: 1665 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_030731.3, NP_109656.1
• RefSeq Size: 4258 bp
• RefSeq ORF: 1665 bp
• Locus ID: 81003
• UniProt ID: Q8BGX0
• Cytogenetics: 13 D1
• Gene Summary: Acts as an E3 ubiquitin-protein ligase. Plays an essential role in autophagy activation during viral infection. Mechanistically, activates TANK-binding kinase 1/TBK1 by facilitating its dimerization and ability to phosphorylate the selective autophagy receptor SQSTM1. In order to achieve this function, TRIM23 mediates 'Lys-27'-linked auto-ubiquitination of its ADP-ribosylation factor (ARF) domain to induce its GTPase activity and its recruitment to autophagosomes.[UniProtKB/Swiss-Prot Function]