Pxn (NM_011223) Mouse Untagged Clone

CAT#: MC218957

Pxn (untagged) - Mouse paxillin (Pxn), transcript variant alpha, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Pxn
• Synonyms: AW108311; AW123232; Pax
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC218957 representing NM_011223
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGACGACCTCGATGCCCTGCTGGCAGACTTGGAATCTACCACCTCCCACATCTCCAAACGGCCAGTGT
  TCTTGTCAGAGGAGCCCCCCTACTCCTACCCAACTGGAAACCACACATACCAGGAGATTGCGGTGCCACC
  TCCGGTCCCACCACCACCGTCCAGCGAGGCCCTCAATGGCACGGTCCTGGACCCTTTAGATCAGTGGCAG
  CCTAGTGGTTCCAGATACGCTCACCAGCAGCCTCCGTCCCCACTGCCCGTGTACAGCTCCAGTGCTAAAA
  ATTCCAGTGCCTCCAACACCCAGGACGGCGTGGGCTCCCTTTGTTCCCGAGCAGGCGAGGAAGAGCACGT
  CTACAGCTTCCCCAACAAGCAGAAGTCGGCAGAGCCCTCACCTACCGTCATGAGCTCCTCCCTGGGCAGC
  AACCTCTCTGAACTTGACCGGCTGTTACTGGAGCTGAACGCAGTGCAGCACAGCCCGCCTGGCTTCCCTG
  CAGATGAAGCCGAATCGAGCCCCCCCTTGCCTGGAGCCCTGAGCCCCCTTTATGGCATCCCAGAAAATAA
  CACTCCCTTGGGGGGCAAAGCGGGGCCCCTGGTGAAAGAGAAGCCAAAGCGAAATGGAGGCCGGGGGCTG
  GAGGATGTGCGGCCCAGCGTGGAGAGCCTCTTGGATGAACTGGAGAGTTCCGTGCCCAGCCCTGTCCCGG
  CCATCACTGTGAACCAGGGAGAGATGAGCAGTCCGCAGCGAGTCACCTCCAGCCAGCAGCAGACCCGGAT
  CTCGGCCTCCTCTGCCACCAGGGAGCTGGACGAGCTCATGGCGTCACTGTCAGATTTCAAGTTCATGGCC
  CAGGGGAAAACTGGGAGCAGCTCACCCCCTGGGGGACTCTCCAAGCCCGGGAGCCAGCTGGACAGCATGT
  TGGGGAGTCTGCAGTCCGACCTGAACAAGCTAGGGGTCGCCACCGTTGCCAAAGGGGTCTGTGGAGCCTG
  CAAGAAGCCCATCGCCGGGCAGGTCGTGACCGCCATGGGGAAGACATGGCACCCCGAGCACTTTGTGTGC
  ACCCACTGCCAGGAGGAGATTGGATCCCGAAACTTTTTTGAGCGGGACGGACAGCCCTACTGTGAGAAGG
  ACTACCACAGCCTCTTCTCCCCACGCTGCTACTACTGCAACGGACCCATCCTGGATAAAGTGGTGACAGC
  GCTGGACCGCACATGGCACCCGGAACACTTCTTCTGTGCCCAGTGTGGAGCCTTCTTTGGTCCAGAAGGC
  TTCCACGAGAAGGACGGCAAAGCGTACTGTCGTAAAGATTACTTCGACATGTTTGCTCCCAAGTGCGGCG
  GCTGCGCCCGTGCCATCCTGGAGAACTACATTTCAGCCCTCAACACCCTCTGGCACCCCGAATGCTTTGT
  GTGCAGGGAGTGCTTCACACCCTTCGTCAACGGCAGCTTCTTTGAGCACGACGGGCAGCCGTACTGTGAG
  GTCCACTACCACGAGCGGCGCGGCTCGCTGTGCTCCGGCTGCCAGAAGCCCATCACGGGCCGCTGCATCA
  CCGCCATGGCCAAGAAGTTCCACCCCGAGCATTTCGTGTGCGCCTTTTGCCTGAAGCAGCTCAACAAGGG
  CACCTTCAAGGAGCAGAACGACAAACCCTACTGCCAGAGCTGCTTCGTGAAGCTCTTCTGCTAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_011223
• Insert Size: 1674 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_011223.3, NP_035353.1
• RefSeq Size: 3722 bp
• RefSeq ORF: 1674 bp
• Locus ID: 19303
• UniProt ID: Q8VI36
• Cytogenetics: 5 56.1 cM
• Gene Summary: Cytoskeletal protein involved in actin-membrane attachment at sites of cell adhesion to the extracellular matrix (focal adhesion).[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (alpha) lacks an alternate in-frame exon in the 5' coding region, compared to variant beta. It encodes isoform alpha, which lacks an internal segment and is shorter, compared to isoform beta.