Cpeb1 (NM_007755) Mouse Untagged Clone

CAT#: MC218939

Cpeb1 (untagged) - Mouse cytoplasmic polyadenylation element binding protein 1 (Cpeb1), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Cpeb1
• Synonyms: AU024112; Cpe-bp1; Cpeb; mCPEB; mCpeb-1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC218939 representing NM_007755
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCTTTCTCTCTGGAAGAAGCAGCAGGAAGAATAAAGGATTGCTGGGACAACCAAGAAGTTCCTGCAC
  TCTCTACATGTAGCAATGCCAATATCTTTCGAAGGATAAATGCCATTTTGGATGATTCACTGGACTTCAG
  TAAAGTCTGTACAACACCTATAAATCGAGGAATTCATGATCAGTTGCCAGACTTCCAGGACTCTGAAGAA
  ACAGTTACAAGCAGGATGCTTTTCCCCACCTCTGCGCAAGAATCTCCCCGTGGCCTCCCAGATGCAAATG
  GCTTGTGCCTTGGCCTGCAGTCCCTCAGTCTGACTGGCTGGGACCGACCCTGGAGCACCCAGGACTCAGA
  CTCCTCAGCCCAGAGCAGCACACAGTCAGTATTAAGCATGCTCCAAAATCCACTGGGAAATGTCTTAGGA
  AAAGCCCCCTTGAGCTTCCTGTCTCTGGATCCCCTTGGGTCTGACTTGGACAAGTTCCCAGCACCCTCAG
  TTAGAGGATCTCGCCTGGACACCCGGCCCATCCTGGACTCCCGTTCTAGCAGCCCCTCTGACTCAGACAC
  GAGTGGCTTCAGCTCTGGATCAGACCATCTCTCAGATTTGATTTCAAGCCTTCGCATTTCCCCTCCTCTG
  CCCTTTCTTTCTATGACGGGAAATGGTCCCAGAGACCCTCTAAAGATGGGCGTTGGGTCCCGAATGGACC
  AAGAACAAGCTGCTCTTGCTGCAGTTGCTCCCTCTCCAACCAGTGCTCCAAAGAGATGGCCAGGAGCTTC
  TGTGTGGCCATCTTGGGACCTTCTTGGAGCTCCTAAAGACCCATTCAGCATAGAGAGAGAGGCTAGACTC
  CACCGGCAGGCTGCAGCTGTGAATGAAGCTACGTGTACCTGGAGTGGCCAGCTTCCTCCCCGGAACTATA
  AGAACCCCATCTACTCATGCAAGGTGTTCCTAGGAGGCGTTCCTTGGGATATTACAGAAGCTGGATTGGT
  TAACACCTTCCGTGTTTTTGGCTCTCTGAGTGTGGAGTGGCCTGGTAAGGATGGCAAGCACCCCCGGTGT
  CCTCCCAAAGGTAATATGCCTAAAGGGTATGTGTATTTGGTGTTTGAACTAGAGAAGTCCGTCCGGGCCT
  TGCTTCAGGCTTGTTCTCATGACCCGCTGAGCCCAGATGGCCTGAGTGAATATTATTTCAAGATGTCCAG
  CCGAAGGATGCGCTGCAAAGAGGTACAGGTAATCCCTTGGGTCTTGGCTGACAGCAACTTTGTCTGGAGC
  CCATCTCAGAGGCTGGATCCCAGCAGGACGGTGTTTGTTGGTGCCTTGCACGGGATGCTCAATGCTGAGG
  CTCTGGCTGCCATCTTGAATGACCTATTTGGTGGAGTGGTGTATGCTGGGATTGACACAGATAAGCACAA
  GTACCCCATTGGGTCTGGTCGTGTGACTTTCAATAACCAACGTAGTTATCTGAAAGCAGTCACCGCTGCT
  TTTGTGGAGATCAAAACCACCAAGTTTACCAAGAAGGTTCAGATTGACCCCTATCTAGAAGATTCTCTGT
  GTCTTATCTGCAGCTCACAACCTGGTCCTTTCTTCTGCCGAGATCAGGTCTGCTTCAAGTACTTTTGCAG
  AAGCTGCTGGCACTGGCGACACAGCATGGAAGGCCTACGCCACCATAGCCCCCTAATGAGGAACCAGAAG
  AACTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_007755
• Insert Size: 1686 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_007755.5, NP_031781.1
• RefSeq Size: 3138 bp
• RefSeq ORF: 1686 bp
• Locus ID: 12877
• UniProt ID: P70166
• Cytogenetics: 7 D3
• Gene Summary: Sequence-specific RNA-binding protein that regulates mRNA cytoplasmic polyadenylation and translation initiation during oocyte maturation, early development and at postsynapse sites of neurons. Binds to the cytoplasmic polyadenylation element (CPE), an uridine-rich sequence element (consensus sequence 5'-UUUUUAU-3') within the 3' UTR of mRNAs. In absence of phosphorylation and in association with TACC3 is also involved as a repressor of translation of CPE-containing mRNA; a repression that is relieved by phosphorylation or degradation (By similarity). Involved in the transport of CPE-containing mRNA to dendrites; those mRNAs may be transported to dendrites in a translationally dormant form and translationally activated at synapses. Its interaction with APLP1 promotes local CPE-containing mRNA polyadenylation and translation activation. Induces the assembly of stress granules in the absence of stress (By similarity). Required for cell cycle progression, specifically for prophase entry (By similarity).[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (2) uses a different splice in the coding region, compared to variant 1. The resulting protein (isoform 2) is shorter when it is compared to isoform 1.