Npnt (NM_001029836) Mouse Untagged Clone

CAT#: MC218928

Npnt (untagged) - Mouse nephronectin (Npnt), transcript variant 2, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Npnt
• Synonyms: 1110009H02Rik; AA682063; AI314031; Nctn; POEM
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC218928 representing NM_001029836
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCTGTGCTCCTAGCGGCGGTGCTCGCGTCCTCGCTCTACCTGCAGGTGGCTGCCGACTTCGACGGGA
  GGTGGCCCAGGCAAATAGTTTCTTCAATCGGCCTATGTCGCTATGGAGGCAGGATTGACTGCTGCTGGGG
  CTGGGCCCGCCAGTCCTGGGGACAGTGCCAACCTGTGTGTCAACCACAGTGCAAACACGGAGAGTGCGTC
  GGGCCAAACAAGTGCAAATGTCACCCTGGATTTGCTGGGAAAACCTGCAACCAAGATTTAAACGAGTGTG
  GCCTGAAGCCTCGGCCCTGTAAGCACAGGTGCATGAACACTTTCGGCAGCTACAAGTGCTACTGTCTCAA
  CGGATACATGCTGCTGCCAGACGGGTCCTGCTCAAGTGCCCTATCGTGTTCCATGGCAAACTGTCAGTAT
  GGCTGTGACGTGGTCAAAGGACAGGTCCGATGTCAGTGTCCATCCCCTGGCCTGCAGCTAGCTCCCGATG
  GGAGGACCTGTGTGGATATTGATGAATGTGCGACTGGAAGAGTCTCCTGCCCTCGATTTAGGCAGTGTGT
  CAACACGTTTGGGAGCTACATCTGCAAGTGTCACACTGGTTTCGACCTCATGTACATTGGAGGCAAATAT
  CAGTGCCATGACATCGACGAGTGCTCTCTTGGACAGCACCAGTGTAGCAGCTATGCCCGGTGTTACAACA
  TACATGGGTCCTACAAGTGCCAATGTAGAGATGGATACGAGGGGGATGGACTGAACTGTGTGTATATCCC
  CAAAGTCATGATTGAACCTTCAGGTCCAATCCATATGCCAGAAAGAAATGGTACAATCTCAAAGGGTGAT
  GGAGGACATGCGAATAGGATTCCTGATGCTGGAAGTACAAGGTGGCCCCTGAAGACACCATATATTCCTC
  CTGTCATTACCAACAGGCCTACTTCCAAGCCAACAACAAGACCTACACCAAACCCAACACCACAGCCTAC
  TCCACCACCTCCACCACCCCTCCCGACAGAGCCCAGAACAACTCCACTACCACCAACCCCAGAAAGGCCA
  TCTACCAGACCCACCACTATAGCACCTGCTACCAGTACCACTACACGAGTAATTACGGTTGACAACAGGA
  TACAGACGGATCCTCAGAAACCCAGAGGAGATGTGTTCATTCCACGGCAGCCGACAAATGACCTGTTTGA
  GATATTTGAAATCGAAAGAGGGGTCAGCGCGGATGAGGAAGTAAAGGACGACCCAGGTATTCTCATACAC
  AGCTGCAATTTTGACCATGGACTCTGTGGATGGATCAGAGAAAAAGATAGTGACTTGCACTGGGAGACAG
  CCAGGGACCCAGCAGGTGGACAGTATCTCACAGTGTCTGCAGCCAAAGCCCCGGGGGGAAAAGCCGCTCG
  CTTGGTGTTACGTCTCGGCCACCTCATGCATTCAGGGGACCTGTGCCTGTCCTTTAGGCACAAGGTGACT
  GGGCTGCACTCGGGCACACTGCAGGTGTTTGTGAGGAAACACGGTACCCACGGAGCAGCCCTGTGGGGAA
  GAAATGGTGGCCATGGCTGGAGGCAAACCCAGATCACCTTGCGAGGGGCTGACGTCAAAAGCGTCATCTT
  CAAAGGTGAAAAAAGGCGTGGTCACACGGGGGAGATTGGATTGGATGATGTGAGCTTGAAAAGAGGTCGC
  TGCTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001029836
• Insert Size: 1686 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001029836.2, NP_001025007.1
• RefSeq Size: 4619 bp
• RefSeq ORF: 1686 bp
• Locus ID: 114249
• UniProt ID: Q91V88
• Cytogenetics: 3 G3
• Gene Summary: Functional ligand of integrin alpha-8/beta-1 in kidney development. Regulates the expression of GDNF with integrin alpha-8/beta-1 which is essential for kidney development. May also play a role in the development and function of various tissues, regulating cell adhesion, spreading and survival through the binding of several integrins.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (2) lacks two in-frame exons in the 5' coding region compared to variant 3. The encoded isoform (b) is shorter than isoform c. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.