Spns2 (NM_153060) Mouse Untagged Clone

CAT#: MC218881

Spns2 (untagged) - Mouse spinster homolog 2 (Drosophila) (Spns2), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Spns2
• Synonyms: MGC37865
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC218881 representing NM_153060
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGATGTGCCTGGAATGCGCCTCGGCGGCGGCGGGCGGCGCGGAGGAGGAGGAGGCGGACGCCGAGCGGC
  GGCGCCGGCGCCGGGGGGCGCAGCCAGGGGCTGGAGGTAGCGCTTGCTGCGGGGCGCGGGGTGTTGGGGG
  CGCTGGAGTCGTGTCCGCCGACGAAGAGGTGCAGACGTTGTCCGGCAGCGTGAGGCGGGTCCCGTCCGGA
  CTCCCCAGCATCCCCAGCACCCCTGGCTGCGCAGCAGCTGCCAAGGGCCCCAGCGCGCCGCAGCCCAAAC
  CGGCCAGCTTAGGCCGAGGGCGGGGGGCAGCTGCTGCCATCCTGAGTTTAGGCAACGTGCTCAACTACCT
  GGACAGGTACACTGTGGCAGGTGTTCTTCTGGACATCCAGCAGCACTTTGGGGTCAAGGACCGGGGCGCC
  GGCTTGCTGCAGTCAGTGTTCATCTGCAGCTTCATGGTGGCTGCCCCCATCTTTGGCTACCTGGGCGACC
  GCTTCAATAGAAAGGTGATCCTCAGCTGTGGCATCTTCTTCTGGTCTGCTGTCACCTTCTCGAGTTCTTT
  CATCCCTCAGCAGTACTTCTGGCTACTAGTCCTGTCCCGAGGGCTAGTAGGTATTGGTGAAGCCAGCTAC
  TCCACCATAGCACCCACCATCATTGGCGACTTGTTTACCAAGAACACACGCACATTGATGCTATCTGTCT
  TCTATTTTGCCATCCCCCTGGGCAGTGGCCTGGGCTATATCACAGGTTCCAGCGTGAAGCAGGCAGCTGG
  AGACTGGCATTGGGCCCTGCGGGTTTCCCCCGTCCTGGGCATGATCACAGGAACACTCATCCTCATCTTG
  GTCCCAGCCACTAAGAGAGGCCATGCTGATCAACTTGGGGGGCAGCTCAAAGCACGGACCTCCTGGCTTC
  GAGACATGAAGGCCCTGATCCGAAACCGCAGTTACGTCTTCTCCTCCCTGGCCACATCCGCTGTGTCCTT
  CGCCACAGGGGCCCTGGGCATGTGGATTCCCCTCTATCTTCACCGTGCTCAAGTTGTACAAAAGACAGCA
  GAGACTTGCAACAGCCCGCCCTGTGGAGCCAAAGACAGCCTCATCTTTGGAGCCATTACCTGCTTTACTG
  GCTTCCTGGGCGTAGTCACGGGCGCGGGAGCCACTCGTTGGTGCCGCCTGCGGACTCAACGTGCTGACCC
  ATTGGTGTGTGCTGTGGGCATGCTGGGATCCGCCATCTTCATCTGCCTCATCTTTGTGGCTGCCAAGACC
  AGCATTGTAGGCGCTTATATCTGCATTTTTGTTGGAGAGACACTGCTGTTTTCTAACTGGGCCATCACTG
  CAGACATCCTCATGTATGTGGTCATCCCCACTCGGCGAGCCACTGCTGTGGCCCTGCAGAGCTTCACCTC
  CCATCTGCTGGGGGACGCTGGAAGCCCCTACCTCATTGGCTTTATCTCGGACCTAATCCGCCAGAGCACC
  AAGGACTCCCCGCTCTGGGAGTTCCTGAGCCTGGGCTATGCCCTCATGCTGTGCCCTTTTGTCGTGGTCC
  TGGGTGGCATGTTCTTCCTTGCCACTGCTCTCTTCTTCCTCAGCGACCGTGCCAAGGCTGAGCAGCAGGT
  GAACCAGCTGGTGATGCCTCCTGCATCCGTGAAAGTCTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_153060
• Insert Size: 1650 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_153060.2, NP_694700.2
• RefSeq Size: 3257 bp
• RefSeq ORF: 1650 bp
• Locus ID: 216892
• UniProt ID: Q91VM4
• Cytogenetics: 11 B4
• Gene Summary: Sphingolipid transporter required for migration of myocardial precursors. Transports sphingosine 1-phosphate (S1P), a secreted lipid mediator that plays critical roles in cardiovascular, immunological, and neural development and function. Mediates the export of S1P from cells in the extraembryonic yolk syncytial layer (YSL), thereby regulating myocardial precursor migration (By similarity).[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments.