Fip1l1 (NM_024183) Mouse Untagged Clone

CAT#: MC218835

Fip1l1 (untagged) - Mouse FIP1 like 1 (S. cerevisiae) (Fip1l1), transcript variant 2, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Fip1l1
• Synonyms: 1300019H17Rik; Rje
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC218835 representing NM_024183
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCGGCCGGCGAGGTGGAGCGCCTGGTGGAGCTGAGCGGCGGGACCGGAGGGGATGAGGAGGAAGAGT
  GGCTGTATGGCGGCCCGTGGGACGTGCATGTGCACAGTGACTTGGCAAAGGATCTAGATGAGAATGAAGT
  TGAAAGGCCAGAAGAAGAAAATGCCAGTGCTAATCCTCCATCTGGAATTGAAGAAGAAGCTGCTGAAAAT
  GGCGTGGCAAAACCGAAAGTGACAGAGACTGAAGACGACAGTGATAGTGACAGTGACGATGATGAAGATG
  ATGTTCACGTCACTATAGGAGACATCAAAACAGGAGCACCACAGTATGGGAGTTATGGAACAGCACCAGT
  AAATCTTAATATCAAGGCAGGGGGAAGAGTTTATGGAAATACAGGAACCAAAGTCAAAGGAGTGGACCTC
  GATGCACCTGGCAGCATTAATGGAGTTCCACTCTTGGAAGTAGATCTGGATTCTTTTGAAGATAAACCAT
  GGCGGAAACCTGGTGCTGATCTTTCTGATTATTTTAACTATGGCTTTAATGAAGATACTTGGAAAGCTTA
  CTGTGAAAAACAAAAGAGGATACGAATGGGACTGGAAGTTATACCTGTTACTTCAACCACAAACAAGATT
  ACGGTACAGCAAGGGAGAACTGGAAATTCAGAGAAAGAAGCAGCACTTCCATCTACAAAAGCTGAGTTTA
  CTTCTCCGCCATCATTGTTCAAGACTGGTCTGCCACCAAGCAGGAGATTACCTGGAGCAATTGATGTCAT
  TGGTCAAACAATAACCATCAGCCGAGTGGAAGGAAGACGGCGTGCAAATGAGAACAGCAATATACAGGTC
  CTTTCTGACAGATCTGCCACTGAAGTAGACAACAATTTCAGCAAACCACCTCCATTTTTCCCTCCAGGGG
  CTCCTCCTACTCACCTTCCTCCTCCTCCATTTCTCCCACCACCTCCAACTGTCAGCACCGCCCCACCTCT
  CATTCCACCACCAGGTATTCCAATAACTGTACCACCTCCAGGTTTCCCCCCTCCACCAGGCGCCCCACCT
  CCATCTCTTATACCAACAATAGAAAGTGGCCATTCCTCCGGGTATGACAGCCGTTCTGCACGAGCGTTTC
  CCTATGGCAATGTCGCCTTTCCCCACCTTACCAGTTCTGCTCCCTCATGGCCGAGCCTTGTGGACACCAC
  CAAGCAGTGGGACTATTATGCAAGGAGAGAAAAAGACCGAGACCGAGACCGGGAGAGAGACCGAGACAGA
  GAGCGAGAGCGGGACCGGGACAGAGAAAGGGAGCGCACCCGGGAGCGGGAGCGAGAGCGCGACCACAGCC
  CCACCCCAAGTGTTTTCAACAGTGACGAAGAGCGATACAGATACAGGGAGTATGCAGAGAGAGGCTATGA
  GCGCCATAGAGCTAGTCGGGAGAAAGAAGAGCGGCACAGAGAACGACGTCACAGAGAGAAGGAGGAAACA
  AGACACAAGTCCTCTCGCAGTAATAGTAGACGTCGCCATGAAAGTGAAGAAGGGGACAGTCACAGAAGAC
  ACAAGCACAAAAAGTCTAAAAGAAGCAAAGAAGGAAAAGAGGCTGGCAGCGAGCCGGTCCCAGAACAGGA
  GAGCACCGAAGCCGCCCCTGCCGAGTAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_024183
• Insert Size: 1638 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_024183.5, NP_077145.2
• RefSeq Size: 2974 bp
• RefSeq ORF: 1638 bp
• Locus ID: 66899
• UniProt ID: Q9D824
• Cytogenetics: 5 C3.3
• Gene Summary: Component of the cleavage and polyadenylation specificity factor (CPSF) complex that plays a key role in pre-mRNA 3'-end formation, recognizing the AAUAAA signal sequence and interacting with poly(A) polymerase and other factors to bring about cleavage and poly(A) addition. FIP1L1 contributes to poly(A) site recognition and stimulates poly(A) addition. Binds to U-rich RNA sequence elements surrounding the poly(A) site. May act to tether poly(A) polymerase to the CPSF complex (By similarity).[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (2) lacks an alternate in-frame exon in the coding region, compared to variant 1. This results in a shorter protein (isoform 2), compared to isoform 1.