Gtf2h1 (NM_008186) Mouse Untagged Clone

CAT#: MC218806

Gtf2h1 (untagged) - Mouse general transcription factor II H, polypeptide 1 (Gtf2h1), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Gtf2h1
• Synonyms: 62kDa; AW743425; AW822074; BTF2 p62; C77871; p62
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC218806 representing NM_008186
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGACTTCATCAGAAGAAGTGTTGCTGATTGTGAAGAAGGTTCGTCAGAAGAAGCAGGATGGAGCAC
  TGTACCTCATGGCAGAGAGAATTGCTTGGGCACCTGAAGGCAAAGACAGATTTACAATCAGCCATATGTA
  TGCAGATATTAAATGCCAGAAAATCAGTCCAGAGGGAAAAGCTAAAATACAACTTCAGCTGGTCCTGCAT
  GCAGGGGACACAACAAACTTCCATTTTTCCAACGAAAGCACGGCAGTGAAAGAACGGGATGCAGTGAAGG
  ACCTCCTTCAGCAGCTGCTGCCCAAGTTCAAGCGGAAAGCTAATAAAGAGCTGGAGGAGAAGAACAGAAT
  GCTCCAAGAAGATCCTGTTTTATTCCAACTCTATAAAGACCTTGTTGTGAGCCAAGTGATCAGTGCTGAG
  GAATTCTGGGCCAATCGTTTAAATGTGAATGCAACAGATAGTTCTACATCCAGTCACAAGCAGGATGTTG
  GTATTTCTGCAGCATTTCTGGCTGATGTCCGGCCCCAAACGGATGGCTGTAATGGTCTGAGATATAATTT
  AACTTCTGATATCATTGAATCTATATTTAGGACCTATCCAGCAGTAAAAATGAAATATGCAGAAACTGTA
  CCACACAACATGACAGAAAAGGAGTTCTGGACACGCTTTTTCCAGTCCCATTATTTTCACAGGGACCGAC
  TAAATACAGGATCGAAGGACCTCTTTGCAGAATGTGCCAAAATAGATGAGAAGGGATTAAAAACAATGGT
  TTCATTAGGAGTGAAAAACCCAATGCTAGATTTGACATCGTTGGAAGATAAGCCATTAGACGAGGGCTAT
  GGCATTTCCTCTGTGCCATCTACTTCCAATTCCAAATCCATAAAGGAGAATAGTAATGCTGCCATCATCA
  AAAGGTTTAACCACCACAGTGCCATGGTCCTGGCTGCTGGTCTCAGGAAACAACAAGCACAAAATGGACA
  GAATGGTGAGCCCAGCAGCGTGGATGGGAATTCTGGGGATACAGACTGCTTTCAGCCAGCAGTCAAAAGG
  GCAAAGTTGCAGGAGTCCATTGAATATGAAGACTTGGGAAACAACAATTCTGTGAAAACGATTGCACTGA
  ATCTCAAGAAGTCAGACAGGTATTACCATGGTCCTACTCCAATTCAGTCACTGCAGTATGCAACAAGTCA
  GGACATTATTAACTCTTTTCAAAGTATTAGACAAGAAATGGAAGCTTACACACCCAAGTTAACTCAGGTT
  CTCTCAAGCAGTGCTGCTAGTAGCACCATCACAGCCCTGTCACCTGGAGGAGCTCTTATGCAGGGAGGGA
  CACAGCAAGCCGTAAACCAGATGGTGCCAAACGATATTCAGTCTGAATTGAAACACTTGTATGTGGCTGT
  TGGGGAACTTCTGCGGCATTTTTGGTCTTGCTTTCCTGTTAATACACCATTCCTAGAAGAAAAGGTAGTG
  AAAATGAAGAGTAATTTGGAACGGTTCCAAGTTACAAAGCTCTGCCCATTCCAAGAAAAGATTCGGAGGC
  AGTATTTAAGCACAAATCTGGTAAGTCACATAGAAGAGATGCTGCAGACAGCCTACAACAAGCTCCACGC
  GTGGCAATCCCGGCGCCTGATGAAGAAGACGTGA
  
  AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC
  TGGATTACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-RsrII
• ACCN: NM_008186
• Insert Size: 1644 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_008186.4, NP_032212.3
• RefSeq Size: 2749 bp
• RefSeq ORF: 1644 bp
• Locus ID: 14884
• UniProt ID: Q9DBA9
• Cytogenetics: 7 B3
• Gene Summary: Component of the general transcription and DNA repair factor IIH (TFIIH) core complex, which is involved in general and transcription-coupled nucleotide excision repair (NER) of damaged DNA and, when complexed to CAK, in RNA transcription by RNA polymerase II. In NER, TFIIH acts by opening DNA around the lesion to allow the excision of the damaged oligonucleotide and its replacement by a new DNA fragment. In transcription, TFIIH has an essential role in transcription initiation. When the pre-initiation complex (PIC) has been established, TFIIH is required for promoter opening and promoter escape. Phosphorylation of the C-terminal tail (CTD) of the largest subunit of RNA polymerase II by the kinase module CAK controls the initiation of transcription.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (2) differs in the 5' UTR and coding sequence compared to variant 3. The resulting isoform (2) is shorter at the N-terminus compared to isoform 3.