Cyp4a12b (NM_172306) Mouse Untagged Clone

CAT#: MC218751

Cyp4a12b (untagged) - Mouse cytochrome P450, family 4, subfamily a, polypeptide 12B (Cyp4a12b), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Cyp4a12b
• Synonyms: BC060945; Cyp4a12
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC218751 representing NM_172306
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGTGCCTCTGCTCTGAGCTCCATCAGATTCCCAGGAAGCATCTCTGAGTACCTTCAAGTAGCCTCTG
  TGCTCAGCCTGCTCCTGCTGCTCTTCAAGACAGCCCAGCTCTACCTGCACAGGCAATGGCTACTCAGCAG
  TACTCAGCAGTTCCCATCCCCACCTTCTCACTGGCTCTTTGGACACAAGATCTTAAAGGACCAGGACCTT
  CAAGATATTCTAACTAGGATTAAGAATTTCCCAAGTGCCTGTCCACAGTGGCTCTGGGGAAGCAAAGTGC
  GCATTCAAGTGTATGACCCTGACTACATGAAGCTGATTCTGGGGAGATCAGACCCAAAAGCTCATGGTTC
  CTACAGATTTCTAGCTCCCTGGATTGGGCGTGGTTTGCTTCTGCTGGATGGACAGACATGGTTTCAGCAC
  CGACGAATGTTGACCCCAGCTTTCCACTATGACATTCTGAAGCCTTACACGGAAATCATGGCAGACTCTG
  TTCATGTCATGCTGGATAAATGGGAACAGATTGTTGGCCAGGATTCCACCCTGGAGATCTTCCAACACAT
  CACCTTGATGACCTTGGACACCATCATGAAGTGTGCCTTCAGCCATGAGGGCAGTGTCCAGTTGGACAGA
  AAATACAAGTCCTATATCCAGGCAGTTGAGGACCTGAACAATCTCTTTTTTCTCCGTGTGCGGAACATCT
  TTCACCAGAATGACATCATCTACAGAGTGTCCTCTAATGGCTGCTTGGCCAACAGTGCCTGCCAACTTGC
  CCATGATCACACAGACCAAGTGATCAAATCAAGGAGGAGTCAACTTCAGGATGAGGAAGAGTTGGAAAAG
  CTTAAGAAGAAAAGGCGATTGGATTTCCTGGACATCCTCCTATTTGCCAGAATGGAAAATGGAAAAAGCT
  TATCTGATAAGGACCTTCGTGCTGAAGTGGATACTTTCATGTTCGAGGGCCATGACACCACAGCTAGTGG
  TATCTCCTGGATCTTCTATGCTTTGGCCACAAATCCTGAACATCAACAGAGATGCAGGAAGGAGATCCAA
  AGTCTCTTAGGAGATGGGGCTTCTATCACCTGGAACGACCTGGACAAGATGCCCTATACTACCATGTGCA
  TCAAGGAGGCCCTGAGGATCTACCCCCCTGTACCAAGTGTGAGCAGAGAGCTCAGCTCACCTGTCACCTT
  TCCTGATGGACGTTCTTTACCCAAAGGTATCCATGTCATGCTCTCCTTTTATGGCCTTCATCACAACCCA
  ACTGTGTGGCCAAATCCAGAGGTGTTTGATCCTTCTCGATTTGCACCAGGGTCTTCCCGGCACAGCCACT
  CATTCCTGCCCTTCTCAGGAGGAGCAAGGAACTGCATTGGGAAACAGTTTGCGATGAATGAGCTGAAGGT
  GGCTGTGGCCCTGACCCTGCTCCGCTTTGAGCTGCTGCCAGATCCCACCAGAGTCCCAATCCCCATACCA
  AGAATTGTGTTGAAGTCCAAGAATGGGATCCACTTGCATCTCAAAAAGCTCCAATAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_172306
• Insert Size: 1527 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_172306.2, NP_758510.2
• RefSeq Size: 2395 bp
• RefSeq ORF: 1527 bp
• Locus ID: 13118
• UniProt ID: A2A974
• Cytogenetics: 4 D1
• Gene Summary: A cytochrome P450 monooxygenase involved in the metabolism of fatty acids and their oxygenated derivatives (oxylipins) (PubMed:17112342). Mechanistically, uses molecular oxygen inserting one oxygen atom into a substrate, and reducing the second into a water molecule, with two electrons provided by NADPH via cytochrome P450 reductase (CPR; NADPH-ferrihemoprotein reductase) (PubMed:17112342). Catalyzes predominantly the oxidation of the terminal carbon (omega-oxidation) of saturated and unsaturated fatty acids (PubMed:17112342). May act as a major omega-hydroxylase for dodecanoic (lauric) acid in kidney (PubMed:17112342). Participates in omega-hydroxylation of (5Z,8Z,11Z,14Z)-eicosatetraenoic acid (arachidonate) to 20-hydroxyeicosatetraenoic acid (20-HETE), a signaling molecule acting both as vasoconstrictive and natriuretic with overall effect on arterial blood pressure (PubMed:17112342). Acts as an omega-hydroxylase and epoxidase toward (5Z,8Z,11Z,14Z,17Z)-eicosapentaenoc acid (EPA). Catalyzes the epoxidation of the last double bond of EPA with no preferred stereoselectivity, producing both (R,S) and (S,R) stereoisomers (PubMed:17112342). Can also catalyze the omega-1 and omega-2 oxidation of fatty acids with lower efficiency (PubMed:17112342).[UniProtKB/Swiss-Prot Function]