Trim46 (BC065049) Mouse Untagged Clone

CAT#: MC218696

Trim46 (untagged) - Mouse tripartite motif protein 46 (cDNA clone MGC:86021 IMAGE:6418851), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Trim46
• Synonyms: TRIFIC
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >BC065049
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAAGAACATGGAGAAGGAACTGCTGTGCCCAGTGTGCCAAGAGATGTACAAGCAGCCACTAGTGCTGC
  CTTGTACCCACAACGTGTGCCAGGCCTGTGCCCGGGAGGTTTTGGGCCAGCAGGGGTACATAGGCCATGG
  TGGGGATCCGAGTTCGGAGCCCACTTCTCCTGCTTCCACCCCTTCTACCCGCAGCCCCCGCCTCTCTCGG
  AGAACTCTCCCCAAGCCAGATCGCTTGGACCGGCTCCTTAAGTCAGGCTTTGGGACATACCCTGGGCGGA
  AGCGTGGTGCCTTGCACCCCCAGACGATCCTATTCCCGTGCCCAGCCTGCCAAGGCGATGTGGAGCTGGG
  GGAGAGGGGCCTTTCCGGGCTTTTCCGAAACCTGACCCTAGAGCGAGTGGTGGAGAGGTACCGCCAGAGT
  GTGAGTGTGGGAGGAGCCATCCTGTGCCAGTTGTGCAAGCCCCCACCACTAGAGGCCACCAAGGGCTGCA
  CAGAGTGTCGAGCTACCTTCTGTAACGAATGCTTCAAGCTCTTCCACCCCTGGGGCACTCAGAAGGCGCA
  GCATGAGCCCACGCTACCCACTCTTTCATTCCGCCCCAAGGGTCTGATGTGTCCAGACCACAAGGAAGAG
  GTGACCCACTACTGTAAGACATGTCAACGACTGGTGTGCCAACTCTGCAGAGTACGGCGTACCCACAGTG
  GGCACAAGATCACACCAGTGCTCAGTGCCTACCAGGCCCTCAAGGATAAGCTAACAAAGAGCCTGGCATA
  CATCTTGGGAAACCAGGACACTGTGCAGACCCAGATTTGTGAGCTGGAAGAGACCATCAGGCACACTGAG
  GTGAGTGGTCAGCAAGCAAAAGAGGAGGTGTCCCAGCTGGTTCGGGGACTAGGGGCTGTGCTGGAAGAGA
  AGCGGGCCTCACTGCTTCAGGCCATTGAAGAATGCCAGCAAGAGCGCTTATCCCGGCTCAGCGCCCAGAT
  CCATGAACACCAGAGCCTGCTGGATGGCTCGGGTCTGGTGGGTTATGCGCAGGAAGTCCTTAAGGAAACA
  GACCAGCCTTGTTTTGTACAAGCAGCCAAACAGCTGCATAACAGGATTGCCCGAGCCACTGAGGCCCTCC
  AGACATTCCGGCCAGCTGCCAGCTCCTCCTTCCGCCACTGCCAGCTGGATGTGGGGCGTGAGATGAAGCT
  GTTGACCGAGCTTAGCTTCCTGAGAGGCTGTGGCCACCGCGGACTCTGCTCCGGAGCACCCCAGTGCCCG
  AGGCGCCCGTCATAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCTGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: BC065049
• Insert Size: 1275 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC065049, AAH65049
• RefSeq Size: 3233 bp
• RefSeq ORF: 1274 bp
• Locus ID: 360213
• Cytogenetics: 3 F1
• Gene Summary: Microtubule-associated protein that is involved in the formation of parallel microtubule bundles linked by cross-bridges in the proximal axon. Required for the uniform orientation and maintenance of the parallel microtubule fascicles, which are important for efficient cargo delivery and trafficking in axons. Thereby also required for proper axon formation, the establishment of neuronal polarity and proper neuronal migration.[UniProtKB/Swiss-Prot Function]