Pgbd1 (BC089360) Mouse Untagged Clone

CAT#: MC218675

Pgbd1 (untagged) - Mouse piggyBac transposable element derived 1 (cDNA clone MGC:102225 IMAGE:30613267), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Pgbd1
• Synonyms: 4921509E05Rik
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >BC089360
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGGCAGACATATCCTCTGCAGAGAGGTGATGAAGCGGCGACATTACTGGAGAATCTAGCCTCGGAAC
  GTGGAGACACAGGGCAGCAGGCTACTCTCTGTGCTCAGGAACAGGACATGCACCTAGTGGTGACAGAGTA
  TCCAGGAGCCTCTATGGAGGAGTATCAGAGCCTCCATCTCCTGCAGTCTTCGGTCACTCCCTGGACAAGA
  GACTTTCCAGAGCTCCCCCTGGGATGTGAGAGAGATTCTCCTTCCCCCACTGAAAAGGCAGTCTCCACTT
  TGAACACTGTCGGGAGCTGCCACCCACCCCATGACTCCTGGGCCAAGATGCACATTGCGTCCTTGCAGTA
  CATTACTCAGAAAGGGAGGGAAAAAGCCAAATTTAGAGTGATCGAGCTGCTCCAAGGCCATGCGTTTTCT
  GATGAGTCAGAGACAGAGGAAGACAATGAGCCTGAGGTACAGCCTGAGAGAAAGAAGATAAAGGCATCCA
  GCATCCCTGAGAAGAGCTGGACCAAACGAGACAGTAAGCCTAACTTTCCAAGCTGGTCAGCCCTGGATTC
  TGGACTTTTGAATCTTAAGAGTGAGAAGTTGAACCCAGTAGAGCTCTTTGAATTATTTTTTGATGGTGAA
  ACATTCAACTTGATTGTCAATGAAACCAATAATTATGCTTCTCAGAAAAATGTCAGCCTAGAAGTCACAG
  TGCAGGAAATGAGGTGTGTGTTTGGTGTTCTGCTCTGCAGTGGATTTGTAAGGCATCCCAGAATGGGGAT
  GTATTGGGAAATCTCTGATTCTGATCAGACCCTGGTTAGAAATGAAATTAGAAGGGACAGATTTGAATTA
  ATTTTCTCATGCCTGCATTTTGCAGATAATAAGCACCTCGATCAAAAAGATAAGTTTTCAAATTTGAGGC
  CTCTCATAAAGCAAATGAAAAATTTTCCTCTTGTATGCCCCCCTGAGGAATACTATTGTTTTGACAAGTC
  AATGTGTGAATGCTTTGATTGTGACCAATTTCTGAATGGGAAGCCTCTTCAAATTGGCTATAAAATTTGG
  TGTGGTACAACTAACTACGCAAGGCTATCTGGTTTGGTTTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCTGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: BC089360
• Insert Size: 1092 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC089360, AAH89360
• RefSeq Size: 2980 bp
• RefSeq ORF: 1091 bp
• Locus ID: 319207
• Cytogenetics: 13 A3.1