Klhl18 (BC025563) Mouse Untagged Clone

CAT#: MC218672

Klhl18 (untagged) - Mouse kelch-like 18 (Drosophila) (cDNA clone MGC:36415 IMAGE:5322893), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Klhl18
• Synonyms: A930041K15, MGC36415
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >BC025563
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGATGTGTGCTGTGTTGTACGATGCAGCCAACAGCTTCATCCACCAGCACTTTGTAGAGGTGTCTCTGT
  CCGAAGAGTTCCTGGCCCTGCCCTTGGAAGACGTGCTTGAGCTGGTGTCCCGGGATGAGCTGAATGTGAA
  GTCAGAGGAGCAGGTTTTTGAAGCTGCATTGGCCTGGGTCAGGTATGACCGGGAGCAGAGGGGACCATGC
  CTGCCCGAGCTGCTGTCCAATATCCGCCTGCCTCTTTGCCGGCCCCAGTTCTTATCAGATCGAGTGCAGC
  AAGATGACCTGGTACGGTGCTGTCACAAATGCAGGGACCTGGTCGATGAAGCAAAGGACTATCATCTGAT
  GCCAGAGCGCCGGCCCCACCTGCCAGCTTTCAGGACTCGGCCCCGATGCTGCACGTCCATCGCTGGGCTC
  ATCTACGCTGTGGGGGGCCTCAACTCAGCAGCAAATTTTTATGCAGGTGATTCCCTGAATGTGGTGGAAG
  TGTTCGACCCTATCGCCAATCGCTGGGAAAAGTGCCATCCCATGACAACAGCCCGAAGCCGTGTGGGTGT
  GGCTGTGGTGAACGGGCTCCTCTATGCTATCGGGGGATATGATGGTCAGTTGCGGCTGAGCACCGTGGAG
  GCCTACAATCCTGAGACGGACACATGGACCCGAGTGGGGAGCATGAATAGCAAGCGAAGTGCCATGGGGA
  CAGTCGTGCTGGATGGACAGATCTACGTGTGTGGAGGCTATGACGGCAACTCCTCCCTCAACTCTGTGGA
  GACCTACTCACCTGAGACGGACAAGTGGACAGTGGTGACTCCGATGAGCTCAAACCGGAGTGCTGCTGGG
  GTGACAGTCTTTGAGGGCAGGATATATGTGTCAGGAGGCCACGATGGCTTGCAGATCTTCAGCAGTGTGG
  AACACTACAACCACCACACGGCCACCTGGCACCCGGCAGCCAGCATGCTCAACAAGCGCTGCCGACACGG
  AGCCGCCTCCCTGGGAAGCAAGATGTTTGTCTGTGGGGGCTATGATGGCTCTGGCTTCCTCAGTATTGCT
  GAGATGTACAGCTCTGTGGCAGACCAGTGGTGTCTCATAGTACCCATGCACACACGCCGGAGCCGGGTCT
  CCCTCGTGGCCAGCTGTGGGCGCCTCTATGCAGTGGGAGGTTACGATGGACAGTCAAACCTAAGCTCAGT
  GGAGATGTACGACCCAGAGACGGACCGCTGGACATTTATGGCCCCCATGGCATGCCACGAGGGGGGGGTT
  GGTGTGGGCTGCATCCCTCTTCTCACCATCTAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCTGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: BC025563
• Insert Size: 1293 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC025563, AAH25563
• RefSeq Size: 4559 bp
• RefSeq ORF: 1292 bp
• Locus ID: 270201
• Cytogenetics: 9 F2
• Gene Summary: Substrate-specific adapter of a BCR (BTB-CUL3-RBX1) E3 ubiquitin-protein ligase complex required for mitotic progression and cytokinesis (By similarity). The BCR(KLHL18) E3 ubiquitin ligase complex mediates the ubiquitination of AURKA leading to its activation at the centrosome which is required for initiating mitotic entry (By similarity). Regulates light- and dark-dependent alpha-transducin localization changes in rod photoreceptors through UNC119 ubiquitination and degradation (PubMed:31696965). Preferentially ubiquitinates the unphosphorylated form of UNC119 over the phosphorylated form (PubMed:31696965). In the presence of UNC119, under dark-adapted conditions alpha-transducin mislocalizes from the outer segment to the inner part of rod photoreceptors which leads to decreased photoreceptor damage caused by light (PubMed:31696965).[UniProtKB/Swiss-Prot Function]