Mppe1 (BC045146) Mouse Untagged Clone

CAT#: MC218593

Mppe1 (untagged) - Mouse metallophosphoesterase 1 (cDNA clone MGC:54864 IMAGE:6306245), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Mppe1
• Synonyms: A530095G11
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >BC045146
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCTCTGGTCAGATGGGGGCTGAAAAGACAGAACTTCCATCCGTTGAGGAGGAGGAGGAGAGCGTTGC
  TGTTGAAACTCACAGTTGTCATTATCTCTGTGCTTCTGTTTTGTGAATATTTCATCTATTACCTGGTCCT
  CTTTCGGTGCCATTGGCCTGAGGTGAAGACTTTAGCCCATGGAGGCAGGCAGGAGCCTGTACTGAAAGCC
  ATGTTTCTGGCTGACACTCACTTACTTGGGGAGATAAGAGGCCATTGGCTGGACAAATTACGAAGGGAGT
  GGCAAATGGAGAGAGCTTTTCAGACAGCTCTGTGGTTGCTGCAACCGGAAGTCGTCTTCATTTTGGGAGA
  CATCTTTGATGAAGGGAAGTGGAGCTCCGATCAGGCCTGGGCAGACGATGTGCAGAGATTTCAGAGGATG
  TTCAGACATGACAGCCATGTGCAGCTGAAGGTGGTCATTGGCAATCATGACGTTGGCTTCCACTATCAGA
  TGAGTAAATACAGAATAAAGCGATTTGAGAAAGTGTTCGGCTCTGAAAGACTGCTCTCTCTGAAAGGAGT
  TAATTTTGTCATGGTCAACAGCGTTGCAATGGAAGGGGATGGTTGTATCATATGCTCTGAAGAGGAAGCT
  GAACTCAGAGAGATCTCTCGTAAACTGAATTGCTCCCAAGAGGTGCCGGGATCCAGTCAGTGTGACCGTG
  AGCCCGAGCCGAGGCTCCCCTTGTCCGCTCCAGTGTTGCTGCAGCACTACCCGCTCTACCGGGCCAGCGA
  TGCTAACTGTTCAGGTGAAGATGCGGCTCCCCCAGAGGAAAGGAACGTCCCCTTTGAGGAGAAATATGAT
  GTTCTTTCTCGGGAGGCCTCACAAAAGCTGTTGTGGTGGCTCCGGCCTCGCCTGGTTCTGAGTGGCCACA
  CACACAGTGCCTGTGAGGTGCTCCACCCAGGAGGAGCCCCTGAGGTGAGCGTCCCATCTTTCAGTTGGAG
  GAACCGAAACAACCCAAGTTTCATCATGGGCAGCCTGACATCCAGAGATTACGCTCTGTCCAAGTGCTAC
  CTCCCGTTTGAGGACACGGTGCTGACCATGTACGGTGCAGCGGCTGGCTTCCTCATGATCCTCATCTTAG
  TTCACTTTGAGCATCTGCCCTCGCCTTTTCTGTGTGGCTGGAAACTGTGCAGGTTGCACATGAGGAGATG
  A
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCTGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: BC045146
• Insert Size: 1191 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC045146, AAH45146
• RefSeq Size: 1715 bp
• RefSeq ORF: 1190 bp
• Locus ID: 225651
• Cytogenetics: 18 E1
• Gene Summary: Metallophosphoesterase required for transport of GPI-anchor proteins from the endoplasmic reticulum to the Golgi. Acts in lipid remodeling steps of GPI-anchor maturation by mediating the removal of a side-chain ethanolamine-phosphate (EtNP) from the second Man (Man2) of the GPI intermediate, an essential step for efficient transport of GPI-anchor proteins (By similarity).[UniProtKB/Swiss-Prot Function]