Rbbp5 (BC057632) Mouse Untagged Clone

CAT#: MC218570

Rbbp5 (untagged) - Mouse retinoblastoma binding protein 5 (cDNA clone MGC:67708 IMAGE:4918318), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Rbbp5
• Synonyms: 4933411J24Rik; C330016J05
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC218570 representing BC057632
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAAATCTGCTCCTGTCATGTTGACCCTTTCAGATTCCAAACATGTTGTTCTGCCGGTAGACGATGACT
  CGGATTTGAACGTGGTGGCATCTTTTGATAGGCGAGGGGAATATATTTATACAGGAAATGCAAAAGGCAA
  GATCTTGGTCCTAAAAACAGACTCTCAGGATCTTGTTGCTTCCTTCAGAGTAACAACTGGGACAAGCAAT
  ACTACCGCCATTAAGTCAATTGAGTTTGCCCGGAAGGGGAGTTGCTTCCTGATTAACACAGCAGATCGAA
  TAATAAGAGTCTATGACGGCAGAGAGATTTTAACGTGTGGAAGGGATGGAGAGCCAGAGCCTATGCAGAA
  GCTGCAGGACTTGGTGAATAGGACTCCATGGAAAAAATGTTGTTTCTCTGGGGATGGGGAGTACATAGTG
  GCGGGCTCTGCGAGGCAGCATGCGCTGTATATCTGGGAAAAGAGCATTGGCAACCTGGTGAAGATCTTAC
  ACGGGACCAGAGGGGAACTCCTGCTGGACGTGGCTTGGCATCCAGTCCGACCCATCATAGCTTCTATCTC
  TAGTGGAGTGGTGTCCATTTGGGCCCAAAATCAAGTAGAAAATTGGAGTGCATTTGCACCAGACTTCAAA
  GAGTTGGATGAAAATGTAGAATATGAGGAAAGAGAATCAGAATTTGATATTGAGGATGAAGATAAGAGTG
  AGCCTGAGCAAACAGGGGCTGATGCTGCTGAAGATGAGGAAGTGGATGTCACCAGCGTGGATCCCATCGC
  TGCCTTCTGTAGCAGTGATGAAGAGCTGGAAGATTCAAAGGCTCTATTGTATTTACCCATTGCCCCTGAG
  GTAGAAGACCCTGAAGAAAACCCGTATGGCCCTCCACCGGATGCAGTCCCAAGCTCCTTGATGGATGAAG
  GGGCTAGTTCAGAGAAGAAGAGGCAGTCTTCAGCAGATGGGTCCCAGCCACCAAAGAAGAAACCTAAAAC
  CACCAATATAGAGCTCCAAGGAGTGCCGAATGATGAAGTCCATCCACTACTGGGTGTGAAGGGGGATGGC
  AAATCGAAGAAGAAGCAAGCAGGCCGGCCTAAAGGATCAAAAGCAGGAGGAGCCATCTCAGAACTGCTGT
  GA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: BC057632
• Insert Size: 1122 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC057632, AAH57632
• RefSeq Size: 3399 bp
• RefSeq ORF: 1121 bp
• Locus ID: 213464
• Cytogenetics: 1 E4
• Gene Summary: As part of the MLL1/MLL complex, involved in mono-, di- and trimethylation at 'Lys-4' of histone H3. Histone H3 'Lys-4' methylation represents a specific tag for epigenetic transcriptional activation. In embryonic stem (ES) cells, plays a crucial role in the differentiation potential, particularly along the neural lineage, regulating gene induction and H3 'Lys-4' methylation at key developmental loci, including that mediated by retinoic acid. Does not affect ES cell self-renewal.[UniProtKB/Swiss-Prot Function]