Clk3 (BC085084) Mouse Untagged Clone

CAT#: MC218519

Clk3 (untagged) - Mouse CDC-like kinase 3 (cDNA clone MGC:103028 IMAGE:6309020), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Clk3
• Synonyms: MGC36552
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >BC085084
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCATCACTGTAAGCGATACCGTTCCCCAGAGCCAGACCCATACCTGAGCTACCGCTGGAAGAGGAGGC
  GGTCTTACAGTCGGGAGCATGAAGGTCGACTACGATACCCATCCCGAAGGGAGCCTCCCCCACGGAGATC
  ACGGTCCAGAAGCCATGATCGTATACCCTACCAGCGGAGGTACCGGGAACACCGTGACAGTGATACGTAT
  CGGTGTGAAGAGCGGAGCCCATCTTTTGGAGAGGACTGCTATGGGTCTTCACGTTCTCGACATCGGAGAC
  GGTCACGAGAGAGGGCGCCGTACCGTACCCGCAAGCATGCCCACCACTGTCACAAACGCCGTACCAGGTC
  TTGTAGCAGTGCTTCCTCGAGAAGCCAACAGAGCAGTAAGCGCAGCAGCCGGAGTGTGGAAGATGACAAG
  GAGGGCCACCTGGTGTGCCGGATCGGCGATTGGCTCCAAGAGCGATATGAGATCGTGGGGAACCTGGGTG
  AAGGCACCTTTGGCAAGGTGGTGGAGTGCTTGGACCATGCCAGAGGGAAGTCACAGGTTGCCCTGAAGAT
  CATCCGTAATGTGGGCAAGTATCGGGAAGCTGCTCGTCTAGAAATTAATGTTCTCAAGAAAATCAAGGAG
  AAAGACAAGGAAAATAAGTTCCTTTGTGTCCTGATGTCTGACTGGTTCAACTTCCATGGTCATATGTGCA
  TCGCCTTTGAGCTCCTGGGCAAGAACACCTTTGAGTTCCTGAAGGAGAACAACTTCCAGCCTTACCCCCT
  ACCACATGTCCGGCACATGGCCTACCAGCTCTGTCATGCCCTTAGATTTCTACACGAGAACCAGCTGACC
  CACACAGACTTGAAGCCAGAGAACATCTTGTTTGTGAATTCTGAGTTTGAAACCCTCTACAATGAGCACA
  AGAGCTGCGAGGAGAAGTCAGTGAAGAACACCAGCATCCGAGTGGCAGACTTTGGCAGTGCCACGTTTGA
  CCATGAACATCACACCACCATTGTGGCCACCCGTCACTACCGCCCACCTGAGGTGATCCTTGAGCTGGGC
  TGGGCACAGCCCTGTGATGTCTGGAGTATCGGCTGCATTCTCTTTGAGTACTACCGTGGCTTTACACTCT
  TCCAGACCCATGAAAATAGAGAACACTTGGTTATGATGGAGAAGATTCTAGGACCCATCCCATCACACAT
  GATCCACCGTACCAGGAAGCAGAAATATTTCTACAAAGGGGGCCTGGTTTGGGATGAGAACAGCTCTGAT
  GGGCGGTATGTGAAGGAGAACTGCAAACCTCTGAAGAGTTACATGCTGCAGGACTCCCTGGAGCATGTGC
  AGCTGTTTGACCTGATGAGGAGGATGTTAGAGTTCGACCCTGCTCAGCGCATCACATTGGCAGAAGCCTT
  GCTGCACCCCTTCTTTGCTGGCCTGACCCCTGAGGAGCGGTCCTTCCACAGCAGCCGTAACCCCAGCAGA
  TGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCTGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: BC085084
• Insert Size: 1473 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC085084, AAH85084
• RefSeq Size: 1778 bp
• RefSeq ORF: 1472 bp
• Locus ID: 102414
• Cytogenetics: 9 B
• Gene Summary: Dual specificity kinase acting on both serine/threonine and tyrosine-containing substrates. Phosphorylates serine- and arginine-rich (SR) proteins of the spliceosomal complex. May be a constituent of a network of regulatory mechanisms that enable SR proteins to control RNA splicing and can cause redistribution of SR proteins from speckles to a diffuse nucleoplasmic distribution. Phosphorylates SRSF1 and SRSF3. Regulates the alternative splicing of tissue factor (F3) pre-mRNA in endothelial cells.[UniProtKB/Swiss-Prot Function]