Trip4 (NM_001170907) Mouse Untagged Clone

CAT#: MC218328

Trip4 (untagged) - Mouse thyroid hormone receptor interactor 4 (Trip4), transcript variant 2, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Trip4
• Synonyms: 4930558E03Rik; ASC-1; Asc1; BB191711
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC218328 representing NM_001170907
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGGTGGCTGGGGCGGCGTACCGGGAGCCGCTAGTACACTGGTGCACACAGCAGTTGCAAAAAACTT
  TCGCCCTGGATGTCAGCGAGGAAATCATACAATATGTTTTGTCAATTGAGAATGCTGAAGAAATACGAGA
  ATATGTGACAGACCTTCTCCAGGGAAATGAGGGCAAAAAGGGTCAATTCATAGAAGATCTTATAACCAAA
  TGGCAAAAGAATGATCAAGAGTTCATCTCTGATTCCTTTCAACAGTGCTTGAGAAAAGATGAAATTTTAG
  ATGGACAGAGATCAGTGGACCAGCTAAAGCGGAGTAGGAGGAAAGGACGAAACAAACAGGAAGTTCCTGC
  ATTTCCTGAACCTGACGTAGCTGTGGAGGTCAAAACACCTTTAGATTTGGCTAAGGCACAAGAAAGCAAC
  AACTCCGTAAAGAAGAAGACAAGATTTGTCAATTTATATACAAGAGAGGGACAGGACAAACTTGCAGTCC
  TGCTTCCTGGTCGTCACCCGTGTGATTGCCTGGGCCAGAAGCACAAACTCATCAATAACTGTCTGGTCTG
  TGGGCGGATCGTTTGTGAGCAGGAGGGTTCAGGCCCCTGCTTGTTCTGTGGCTCTTTGGTGTGTACTAAT
  GAAGAACAGGATATTTTACAGCGGGACTCAAACAAAAGTCAGAAACTGCTGAAGAAGCTCATGTCAGGGG
  CAGAGACTTCTGGAAAGGTGGACGTCTCTACCAAGGACCTACTTCCTCATCAAGAATCCCGAATGAAGTC
  TGGTTTGGAGAAGGCTATCAAGCATAAGGAAAAACTGTTAGAGTTCGACAGAACGAGTATCCGAAGGACC
  CAAGTTATTGACGATGAATCAGATTACTTTGCCAGTGATTCTAACCAGTGGCTGTCCAAAGTTGAGCGAG
  AAATGCTGCAGAAACGAGAGGAGGAGCTGAGAGAACTCCGACATGCCTCCAGACTTTCTAAGAAAGTGAC
  CATTGACTTCGCGGGGCGGAAGATCCTAGAAGATGAAAATCCACTAGCAGAGTATCACAGCAGACTAGAT
  GAGACAATACAAGCCATTGCCAGTGGGACTTTGAACCAGTCACTGGTCACGTTGGATAGATCTTGTGAAG
  AGCCTTTGGGAGTTCTGGTAAACCCTAACATGTACCAGGCCTCTCCTCAGTGGGTTGACAACACTGGCTC
  AACCCCACAGAAGAAGACTTCACTCTCGGCAGGACCAAGACTAGAGCCCAGTTTACATCAGCACCAGCTG
  CGAATCCAGGACCAAGAATTCCAGGAAGGCTTTGATGGCGGCTGGTGCCTGTCTATGCATCAGCCCTGGG
  CTTCTCTGCTTGTCAGGGGGATTAAAAGGGTGGAGGGCAGATCCTGGTATACTCCTCACAGAGGAAGACT
  TTGGATAGCAGCCACGGGCAAAAGACCTTCCCCTCAGGAAGTCTCAGAACTCCAGGCTACCTATCGTCTT
  CTTCGTGGGAAAGATGTGGAGTTTCCAAATGACTATCCGTCGGGTTGTCTTCTGGGCTGTGTGGACCTTA
  TTGATTGCTTGTCCCAGAAACAATTTCAGGAGCAGGGAAACTGGATTCCAAGATCCATCAAGGAGCAAAG
  AAGGGGTTAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001170907
• Insert Size: 1620 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001170907.1, NP_001164378.1
• RefSeq Size: 6253 bp
• RefSeq ORF: 1620 bp
• Locus ID: 56404
• UniProt ID: Q9QXN3
• Cytogenetics: 9 C
• Gene Summary: Transcription coactivator which associates with nuclear receptors, transcriptional coactivators including EP300, CREBBP and NCOA1, and basal transcription factors like TBP and TFIIA to facilitate nuclear receptors-mediated transcription. May thereby play an important role in establishing distinct coactivator complexes under different cellular conditions. Plays a role in thyroid hormone receptor and estrogen receptor transactivation (By similarity). Also involved in androgen receptor transactivation (PubMed:12077347). Plays a pivotal role in the transactivation of NF-kappa-B, SRF and AP1. Acts as a mediator of transrepression between nuclear receptor and either AP1 or NF-kappa-B. May play a role in the development of neuromuscular junction (By similarity). May play a role in late myogenic differentiation (PubMed:27008887).[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (2) differs in the 5' UTR and uses an alternate splice site in the 3' coding region, which results in a frameshift, compared to variant 1. The resulting protein (isoform 2) is shorter and has a distinct C-terminus compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.