Smyd1 (NM_001160127) Mouse Untagged Clone

CAT#: MC218142

Smyd1 (untagged) - Mouse SET and MYND domain containing 1 (Smyd1), transcript variant 1, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Smyd1
• Synonyms: 4632404M21Rik; Bop; C78565; Zmynd18
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC218142 representing NM_001160127
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGACAATAGGCAGCATGGAGAACGTGGAGGTCTTCACTTCCGAGGGCAAAGGCAGAGGTCTGAAGGCTA
  CGAAGGAGTTCTGGGCTGCGGATGTCATCTTTGCGGAGAGGGCTTATTCTGCAGTGGTTTTTGACAGCCT
  CATTAACTTCGTGTGCCACACCTGCTTCAAGAGGCAGGAGAAGCTCCATCGCTGCGGGCAGTGCAAGTTC
  GCCCATTACTGCGACCGCACGTGCCAGAAGGATGCTTGGCTGAATCACAAGAACGAGTGCGCTGCCATCA
  AGAAATATGGGAAAGTGCCCAACGAGAACATCAGGCTGGCCGCCCGCATCATGTGGCGGGTGGAGAGAGA
  GGGCACTGGGCTCACAGAGGGCTGCCTGGTGTCCGTGGATGACTTACAGAACCACGTGGAGCACTTTGGG
  GAGGAGGAGCAGAAGGAACTCCGAGTAGACGTGGACACGTTCTTGCAGTACTGGCCACCACAGAGCCAGC
  AGTTCAGCATGCAGTATATCTCACACATCTTTGGTGTGATCAACTGCAACGGTTTCACTCTCAGTGACCA
  GAGAGGGCTACAGGCAGTAGGTGTGGGCATCTTCCCCAACCTGGGCCTGGTGAACCATGATTGCTGGCCA
  AACTGCACTGTCATATTCAACAATGGCAATCATGAGGCAGTGAAATCCATGTTTCACACGCAGATGAGGA
  TTGAGCTCCGGGCCCTGGGCAAGATCTCAGAAGGCGAGGAGCTGACTGTGTCCTACATAGACTTCCTGCA
  CCTCAGTGAGGAGCGCAGGCGGCAGCTGAAGAAACAGTACTACTTTGACTGCTCCTGTGAGCACTGCCAG
  AAGGGGCTGAAGGACGACCTCTTCCTGGCAGCGAAGGAAGACCCCAAGCCCTCCCAGGAAGTGGTGAAGG
  AGATGATACAATTCTCAAAGGATACACTGGAAAAAATAGACAAGGCTCGCTCCGAGGGTTTGTATCACGA
  GGTTGTGAAGCTGTGTCGGGAGTGCCTGGAGAAGCAGGAGCCAGTGTTCGCCGACACCAACCTCTACGTG
  CTTCGGCTGCTCAGCATTGCATCAGAGGTCCTCTCCTACCTCCAGGCCTATGAGGAGGCCTCACATTATG
  CCAGGAGGATGGTTGATGGCTACATGAAACTCTACCACCATAACAATGCCCAACTGGGCATGGCTGTGAT
  GAGGGCAGGGCTTACCAACTGGCATGCTGGTCACATCGAAGTGGGGCATGGGATGATCTGCAAAGCCTAT
  GCTATTCTCCTGGTGACCCATGGACCCTCCCACCCTATCACCAAAGACTTAGAGGCCATGCGGATGCAGA
  CAGAGATGGAGCTGCGTATGTTCCGCCAAAACGAATTCATGTATCACAAGATGCGAGAGGCTGCCCTGAA
  CAACCAGCCCATGCAGGTCATGGCTGAGCCTAGCAATGAACCAGCCCCCGCTCTGTTCCATAAGAAGCAG
  TGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001160127
• Insert Size: 1473 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC076601, AAH76601
• RefSeq Size: 3379 bp
• RefSeq ORF: 1473 bp
• Locus ID: 12180
• UniProt ID: P97443
• Cytogenetics: 6 32.14 cM
• Gene Summary: Methylates histone H3 at 'Lys-4' (H3K4me). Acts as a transcriptional repressor. Essential for cardiomyocyte differentiation and cardiac morphogenesis.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (1) encodes the longer isoform (1).