Ambn (BC087927) Mouse Untagged Clone

CAT#: MC218136

Ambn (untagged) - Mouse ameloblastin (cDNA clone MGC:107285 IMAGE:30504437), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Ambn
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >BC087927
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCAGCATCTAAGATTCCACTTTTCAAAATGAAGGGCCTGATCCTGTTCCTGTCCCTAGTGAAAATGA
  GCCTCGCCGTGCCGGCATTTCCTCAACAACCTGGGGCTCAAGGCATGGCACCTCCTGGCATGGCTAGTTT
  GAGCCTTGAGACAATGAGACAGTTGGGAAGCTTGCAGGGACTCAACGCACTTTCTCAGTATTCTAGACTT
  GGCTTTGGAAAAGCACTTAATAGTTTATGGTTGCACGGACTTCTCCCACCGCATAACTCTTTCCCATGGA
  TAGGACCAAGGGAACATGAAACCCAGCAGTATGAATATTCTTTGCCTGTGCATCCCCCACCTCTCCCATC
  ACAGCCATCCTTGCAGCCTCACCAGCCAGGACTGAAACCCTTCCTCCAGCCCACTGCTGCAACCGGTGTC
  CAGGTCACACCCCAGAAGCCAGGGCCTCAGCCTCCAATGCACCCTGGACAGCTGCCCTTGCAGGAAGGAG
  AGCTGATAGCACCAGATGAGCCGCAGGTGGCACCATCCGAAAACCCACCAACACCTGAGGTACCAATAAT
  GGATTTTGCTGATCCACAATTTCCAACCGTGTTCCAGATCGCCCGTTCAATATCTCGGGGACCAATGGCA
  CACAACAAAGCATCCGCTTTTTACCCAGGAATGTTTTACATGTCTTATGGAGCAAACCAATTGAATGCTC
  CTGCCAGAATTGGCTTCATGAGTTCAGAAGAAATGCCTGGAGAAAGAGGAAGTCCCATGGCCTATGGAAC
  TCTGTTCCCAAGATTTGGAGGCTTCAGGCAAACCCTTAGGAGACTGAATCAGAATTCACCCAAGGGAGGA
  GACTTTACTGTGGAAGTAGATTCCCCAGTATCTGTTACCAAAGGCCCTGAAAAAGGAGAAGGTCCAGAAG
  GCTCTCCACTGCAAGAGGCCAACCCAGGCAAACGGGAAAACCCCGCTCTCCTTTCACAAATGGCACCTGG
  GGCCCATGCAGGACTTCTTGCTTTCCCCAATGACCACATCCCCAGTATGGCAAGGGGTCCTGCAGGGCAA
  AGACTCCTTGGAGTCACCCCTGCAGCTGCAGACCCACTGATCACCCCTGAATTAGCAGAAGTTTATGAAA
  CCTATGGTGCTGATGTTACCACACCCCTGGGTGATGGAGAAGCAACCATGGATATCACCATGTCCCCAGA
  CACTCAGCAGCCACTGCTACCTGGAAACAAAGTGCACCAGCCCCAGGTGCACAACGCATGGCGTTTCCAA
  GAGCCCTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCTGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: BC087927
• Insert Size: 1269 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC087927, AAH87927
• RefSeq Size: 1602 bp
• RefSeq ORF: 1268 bp
• Locus ID: 11698
• Cytogenetics: 5 43.63 cM
• Gene Summary: This gene encodes an extracellular matrix glycoprotein that is involved in the formation of dental enamel. Mice lacking the encoded protein fail to undergo normal ameloblast differentiation and develop enamel. Mice overproducing the product of this gene develop thinner and more porous enamel, with disrupted rod patterns and abnormal crystallites. Alternate splicing of this gene results in multiple transcript variants. [provided by RefSeq, Dec 2014]